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rabbit polyclonal anti-neun  (Millipore)


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    Millipore rabbit polyclonal anti-neun
    Rabbit Polyclonal Anti Neun, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+anti-neun/rabbit+anti+neun/pmc09481920__mmc1-122-89-94
    Average 90 stars, based on 1 article reviews
    rabbit polyclonal anti-neun - by Bioz Stars, 2026-10
    90/100 stars

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    Recombinant:

    Article Title: SETD4 cells contribute to brain development and maintain adult stem cell reservoir for neurogenesis
    Article Snippet: The antibodies used in this study included mouse monoclonal anti-SETD4 (1:100, #sc-514060, Santa Cruz Biotechnology), rabbit polyclonal anti-GFAP (1:1000, #Z0334, Dako), goat polyclonal anti-GFAP (1:1000, #ab53554, Abcam), chicken polyclonal anti-NESTIN (1:1000, #NB100-1604, Novus Biologicals), mouse monoclonal anti-NESTIN (1:500, #MAB353, MilliporeSigma), rabbit polyclonal anti-SOX2 (1:200, #ab97959, Abcam), goat polyclonal anti-SOX2 (1:200, #AF2018, R&D Systems), rabbit monoclonal anti-KI67 (1:200, #ab16667, Abcam), mouse monoclonal antiPCNA (1:500, #ab29, Abcam), chicken polyclonal anti-GFP (1:1000, #ab13970, Abcam), rat monoclonal anti-BrdU (1:200, #ab6326, Abcam), goat polyclonal anti-DCX (1:500, #ab113435, Abcam), rabbit polyclonal anti-OLIG2 (1:500, #AB9610, MilliporeSigma), rabbit polyclonal anti-NEUN (1:1000, #ABN78, MilliporeSigma), mouse monoclonal antiMASH1 (1:200, #556604, BD Biosciences), chicken polyclonal anti-RFP (1:500, #600-901-379, Rockland immunochemicals), rabbit polyclonal anti-H4K20ME3 (1:500, #pAb-057-050, Diagenode), rabbit polyclonal antiH3K9AC (1:500, #ab10812, Abcam).

    Article Title: Interstitial-fluid shear stresses induced by vertically oscillating head motion lower blood pressure in hypertensive rats and humans.
    Article Snippet: Shuhei Murase, Naoyoshi Sakitani, Takahiro Maekawa, Daisuke Yoshino , Kouji Takano, Ayumu Konno , Hirokazu Hirai , Taku Saito , Sakae Tanaka, Keisuke Shinohara, Takuya Kishi, Yuki Yoshikawa , Takamasa Sakai , Makoto Ayaori, Hirohiko Inanami, Koji Tomiyasu, Atsushi Takashima, Toru Ogata , Hirotsugu Tsuchimochi , Shinya Sato, Shigeyoshi Saito, Kohzoh Yoshino, Yuiko Matsuura , Kenichi Funamoto , Hiroki Ochi, Masahiro Shinohara, Motoshi Nagao & Yasuhiro Sawada

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: Compound Sakura Finetek Cat# 4583 Vectashield Mounting Medium with DAPI Vector Laboratories Cat# H-1200-10 Critical commercial assays Jo urn al Pr e-p roo f Phosphorylated Neurofilament H Human ELISA BioVendor Cat# RD191138300R Corticosterone EIA IDS Cat# AC-14F1 BCA Protein Assay Kit Pierce Cat# 23225 Deposited data Raw data This paper https://doi.org/10.3494 5/F52P4M Experimental models: Organisms/strains Sprague-Dawley Rat Charles River Laboratories RRID: MGI: 5651135 Software and algorithms Kinema Tracer System Kissei Comtec http://www.kinematrac er.com/ Odyssey Application Software Version 3.0 LI-COR Biosciences RRID: SCR_014579 NIS Elements Imaging Software Nikon Instruments RRID: SCR_014329; https://calm.ucsf.edu/h ardware-and-softwareimage-analysis ImageJ NIH RRID: SCR_003070 AutoQuant Media Cybernetics RRID: SCR_002465 MetaMorph Automation and Image Analysis Software Molecular Devices RRID: SCR_002368 Adobe Photoshop Adobe RRID: SCR_014199 SPSS Statistics version 26.0 IBM RRID: SCR_016479 Graphpad Prism Graphpad RRID: SCR_002798 Adobe Illustrator Adobe RRID: SCR_010279 Other Infinite Horizon Impactor Precision Systems and Instrumentation IH-0400; https://psiimpactors.co m/product/ih400/ Treadmill Muromachi Kikai MK-680; https://muromachi.com /en/archives/english/19 36/ Biological Amplifier Nihon Kohden NEC Biotop 6R12; https://doi.org/10.1371/ journal.pone.0171937 AD Converter Cambridge Electronic Design Limited Micro 1401; https://ced.co.uk/produ cts/microwaveformio Constant Current Stimulator Digitimer DS7A; https://www.digitimer.c om/product/humanneurophysiology/periph eral-stimulators/ds7ads7ah-hv-currentstimulator/ Jo urn al P e-p roo f Plate Reader Tecan GENios; https://www.tecan.com/ customer-news/geniosan-innovativedetection-device-thatdelivers-flexibility-tothe-molecular-biologylaboratory-2347 Infrared Imaging System LI-COR Biosciences Odyssey Infrared Imaging System; RRID: SCR_023765; https://doi.org/10.1038/ s41598-024-70096-0 Cryostat Leica Biosystems CM1950; RRID: SCR_018061; https://www.leicabiosys tems.com/us/histologyequipment/cryostats/lei ca-cm1950/ Inverted Microscope Nikon Instruments ECLIPSE Ti inverted microscope system; RRID: SCR_021242; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Spinning Disk Confocal Microscope Yokogawa Electric Corporation CSU-22; RRID: SCR_020907; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Jo urn al Pr e-p roo f

    Article Title: Atp13a5 Marker Reveals Pericyte Specification in the Mouse Central Nervous System
    Article Snippet: Rabbit polyclonal anti-NeuN , Millipore Sigma , ABN78.

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: For assessment of lesion pathology in the experimental animal model, the tissue sections of the thoracic spinal cord segments were incubated with Jo urn al Pr e-p ro f 58 diluted primary antibodies: rabbit polyclonal anti-NeuN (1:500; Millipore; Cat# ABN78; RRID: AB_10807945), mouse monoclonal anti-GFAP (1:500; Millipore; Cat# MAB360; RRID: AB_11212597), rabbit monoclonal anti-Vimentin (1:50; Cell Signaling Technology; Cat# 5741; RRID: AB_10695459), and rabbit polyclonal anti-GAP43 (1:500; Millipore; Cat# AB5220; RRID: AB_2107282).

    Article Title: SETD4 cells contribute to brain development and maintain adult stem cell reservoir for neurogenesis
    Article Snippet: The antibodies used in this study included mouse monoclonal anti-SETD4 (1:100, #sc-514060, Santa Cruz Biotechnology), rabbit polyclonal anti-GFAP (1:1000, #Z0334, Dako), goat polyclonal anti-GFAP (1:1000, #ab53554, Abcam), chicken polyclonal anti-NESTIN (1:1000, #NB100-1604, Novus Biologicals), mouse monoclonal anti-NESTIN (1:500, #MAB353, MilliporeSigma), rabbit polyclonal anti-SOX2 (1:200, #ab97959, Abcam), goat polyclonal anti-SOX2 (1:200, #AF2018, R&D Systems), rabbit monoclonal anti-KI67 (1:200, #ab16667, Abcam), mouse monoclonal antiPCNA (1:500, #ab29, Abcam), chicken polyclonal anti-GFP (1:1000, #ab13970, Abcam), rat monoclonal anti-BrdU (1:200, #ab6326, Abcam), goat polyclonal anti-DCX (1:500, #ab113435, Abcam), rabbit polyclonal anti-OLIG2 (1:500, #AB9610, MilliporeSigma), rabbit polyclonal anti-NEUN (1:1000, #ABN78, MilliporeSigma), mouse monoclonal antiMASH1 (1:200, #556604, BD Biosciences), chicken polyclonal anti-RFP (1:500, #600-901-379, Rockland immunochemicals), rabbit polyclonal anti-H4K20ME3 (1:500, #pAb-057-050, Diagenode), rabbit polyclonal antiH3K9AC (1:500, #ab10812, Abcam).

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: Rabbit Polyclonal Anti-NeuN , Millipore , Cat# ABN78; RRID: AB_10807945.

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: For assessment of lesion pathology in the experimental animal model, the tissue sections of the thoracic spinal cord segments were incubated with diluted primary antibodies: rabbit polyclonal anti-NeuN (1:500; Millipore; Cat# ABN78; RRID: AB_10807945 ), mouse monoclonal anti-GFAP (1:500; Millipore; Cat# MAB360; RRID: AB_11212597 ), rabbit monoclonal anti-Vimentin (1:50; Cell Signaling Technology; Cat# 5741; RRID: AB_10695459 ), and rabbit polyclonal anti-GAP43 (1:500; Millipore; Cat# AB5220; RRID: AB_2107282 ).

    Protease Inhibitor:

    Article Title: SETD4 cells contribute to brain development and maintain adult stem cell reservoir for neurogenesis
    Article Snippet: The antibodies used in this study included mouse monoclonal anti-SETD4 (1:100, #sc-514060, Santa Cruz Biotechnology), rabbit polyclonal anti-GFAP (1:1000, #Z0334, Dako), goat polyclonal anti-GFAP (1:1000, #ab53554, Abcam), chicken polyclonal anti-NESTIN (1:1000, #NB100-1604, Novus Biologicals), mouse monoclonal anti-NESTIN (1:500, #MAB353, MilliporeSigma), rabbit polyclonal anti-SOX2 (1:200, #ab97959, Abcam), goat polyclonal anti-SOX2 (1:200, #AF2018, R&D Systems), rabbit monoclonal anti-KI67 (1:200, #ab16667, Abcam), mouse monoclonal antiPCNA (1:500, #ab29, Abcam), chicken polyclonal anti-GFP (1:1000, #ab13970, Abcam), rat monoclonal anti-BrdU (1:200, #ab6326, Abcam), goat polyclonal anti-DCX (1:500, #ab113435, Abcam), rabbit polyclonal anti-OLIG2 (1:500, #AB9610, MilliporeSigma), rabbit polyclonal anti-NEUN (1:1000, #ABN78, MilliporeSigma), mouse monoclonal antiMASH1 (1:200, #556604, BD Biosciences), chicken polyclonal anti-RFP (1:500, #600-901-379, Rockland immunochemicals), rabbit polyclonal anti-H4K20ME3 (1:500, #pAb-057-050, Diagenode), rabbit polyclonal antiH3K9AC (1:500, #ab10812, Abcam).

    Article Title: Interstitial-fluid shear stresses induced by vertically oscillating head motion lower blood pressure in hypertensive rats and humans.
    Article Snippet: Shuhei Murase, Naoyoshi Sakitani, Takahiro Maekawa, Daisuke Yoshino , Kouji Takano, Ayumu Konno , Hirokazu Hirai , Taku Saito , Sakae Tanaka, Keisuke Shinohara, Takuya Kishi, Yuki Yoshikawa , Takamasa Sakai , Makoto Ayaori, Hirohiko Inanami, Koji Tomiyasu, Atsushi Takashima, Toru Ogata , Hirotsugu Tsuchimochi , Shinya Sato, Shigeyoshi Saito, Kohzoh Yoshino, Yuiko Matsuura , Kenichi Funamoto , Hiroki Ochi, Masahiro Shinohara, Motoshi Nagao & Yasuhiro Sawada

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: Compound Sakura Finetek Cat# 4583 Vectashield Mounting Medium with DAPI Vector Laboratories Cat# H-1200-10 Critical commercial assays Jo urn al Pr e-p roo f Phosphorylated Neurofilament H Human ELISA BioVendor Cat# RD191138300R Corticosterone EIA IDS Cat# AC-14F1 BCA Protein Assay Kit Pierce Cat# 23225 Deposited data Raw data This paper https://doi.org/10.3494 5/F52P4M Experimental models: Organisms/strains Sprague-Dawley Rat Charles River Laboratories RRID: MGI: 5651135 Software and algorithms Kinema Tracer System Kissei Comtec http://www.kinematrac er.com/ Odyssey Application Software Version 3.0 LI-COR Biosciences RRID: SCR_014579 NIS Elements Imaging Software Nikon Instruments RRID: SCR_014329; https://calm.ucsf.edu/h ardware-and-softwareimage-analysis ImageJ NIH RRID: SCR_003070 AutoQuant Media Cybernetics RRID: SCR_002465 MetaMorph Automation and Image Analysis Software Molecular Devices RRID: SCR_002368 Adobe Photoshop Adobe RRID: SCR_014199 SPSS Statistics version 26.0 IBM RRID: SCR_016479 Graphpad Prism Graphpad RRID: SCR_002798 Adobe Illustrator Adobe RRID: SCR_010279 Other Infinite Horizon Impactor Precision Systems and Instrumentation IH-0400; https://psiimpactors.co m/product/ih400/ Treadmill Muromachi Kikai MK-680; https://muromachi.com /en/archives/english/19 36/ Biological Amplifier Nihon Kohden NEC Biotop 6R12; https://doi.org/10.1371/ journal.pone.0171937 AD Converter Cambridge Electronic Design Limited Micro 1401; https://ced.co.uk/produ cts/microwaveformio Constant Current Stimulator Digitimer DS7A; https://www.digitimer.c om/product/humanneurophysiology/periph eral-stimulators/ds7ads7ah-hv-currentstimulator/ Jo urn al P e-p roo f Plate Reader Tecan GENios; https://www.tecan.com/ customer-news/geniosan-innovativedetection-device-thatdelivers-flexibility-tothe-molecular-biologylaboratory-2347 Infrared Imaging System LI-COR Biosciences Odyssey Infrared Imaging System; RRID: SCR_023765; https://doi.org/10.1038/ s41598-024-70096-0 Cryostat Leica Biosystems CM1950; RRID: SCR_018061; https://www.leicabiosys tems.com/us/histologyequipment/cryostats/lei ca-cm1950/ Inverted Microscope Nikon Instruments ECLIPSE Ti inverted microscope system; RRID: SCR_021242; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Spinning Disk Confocal Microscope Yokogawa Electric Corporation CSU-22; RRID: SCR_020907; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Jo urn al Pr e-p roo f

    Article Title: Atp13a5 Marker Reveals Pericyte Specification in the Mouse Central Nervous System
    Article Snippet: Rabbit polyclonal anti-NeuN , Millipore Sigma , ABN78.

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: For assessment of lesion pathology in the experimental animal model, the tissue sections of the thoracic spinal cord segments were incubated with Jo urn al Pr e-p ro f 58 diluted primary antibodies: rabbit polyclonal anti-NeuN (1:500; Millipore; Cat# ABN78; RRID: AB_10807945), mouse monoclonal anti-GFAP (1:500; Millipore; Cat# MAB360; RRID: AB_11212597), rabbit monoclonal anti-Vimentin (1:50; Cell Signaling Technology; Cat# 5741; RRID: AB_10695459), and rabbit polyclonal anti-GAP43 (1:500; Millipore; Cat# AB5220; RRID: AB_2107282).

    Article Title: SETD4 cells contribute to brain development and maintain adult stem cell reservoir for neurogenesis
    Article Snippet: The antibodies used in this study included mouse monoclonal anti-SETD4 (1:100, #sc-514060, Santa Cruz Biotechnology), rabbit polyclonal anti-GFAP (1:1000, #Z0334, Dako), goat polyclonal anti-GFAP (1:1000, #ab53554, Abcam), chicken polyclonal anti-NESTIN (1:1000, #NB100-1604, Novus Biologicals), mouse monoclonal anti-NESTIN (1:500, #MAB353, MilliporeSigma), rabbit polyclonal anti-SOX2 (1:200, #ab97959, Abcam), goat polyclonal anti-SOX2 (1:200, #AF2018, R&D Systems), rabbit monoclonal anti-KI67 (1:200, #ab16667, Abcam), mouse monoclonal antiPCNA (1:500, #ab29, Abcam), chicken polyclonal anti-GFP (1:1000, #ab13970, Abcam), rat monoclonal anti-BrdU (1:200, #ab6326, Abcam), goat polyclonal anti-DCX (1:500, #ab113435, Abcam), rabbit polyclonal anti-OLIG2 (1:500, #AB9610, MilliporeSigma), rabbit polyclonal anti-NEUN (1:1000, #ABN78, MilliporeSigma), mouse monoclonal antiMASH1 (1:200, #556604, BD Biosciences), chicken polyclonal anti-RFP (1:500, #600-901-379, Rockland immunochemicals), rabbit polyclonal anti-H4K20ME3 (1:500, #pAb-057-050, Diagenode), rabbit polyclonal antiH3K9AC (1:500, #ab10812, Abcam).

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: Rabbit Polyclonal Anti-NeuN , Millipore , Cat# ABN78; RRID: AB_10807945.

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: For assessment of lesion pathology in the experimental animal model, the tissue sections of the thoracic spinal cord segments were incubated with diluted primary antibodies: rabbit polyclonal anti-NeuN (1:500; Millipore; Cat# ABN78; RRID: AB_10807945 ), mouse monoclonal anti-GFAP (1:500; Millipore; Cat# MAB360; RRID: AB_11212597 ), rabbit monoclonal anti-Vimentin (1:50; Cell Signaling Technology; Cat# 5741; RRID: AB_10695459 ), and rabbit polyclonal anti-GAP43 (1:500; Millipore; Cat# AB5220; RRID: AB_2107282 ).

    Blocking Assay:

    Article Title: SETD4 cells contribute to brain development and maintain adult stem cell reservoir for neurogenesis
    Article Snippet: The antibodies used in this study included mouse monoclonal anti-SETD4 (1:100, #sc-514060, Santa Cruz Biotechnology), rabbit polyclonal anti-GFAP (1:1000, #Z0334, Dako), goat polyclonal anti-GFAP (1:1000, #ab53554, Abcam), chicken polyclonal anti-NESTIN (1:1000, #NB100-1604, Novus Biologicals), mouse monoclonal anti-NESTIN (1:500, #MAB353, MilliporeSigma), rabbit polyclonal anti-SOX2 (1:200, #ab97959, Abcam), goat polyclonal anti-SOX2 (1:200, #AF2018, R&D Systems), rabbit monoclonal anti-KI67 (1:200, #ab16667, Abcam), mouse monoclonal antiPCNA (1:500, #ab29, Abcam), chicken polyclonal anti-GFP (1:1000, #ab13970, Abcam), rat monoclonal anti-BrdU (1:200, #ab6326, Abcam), goat polyclonal anti-DCX (1:500, #ab113435, Abcam), rabbit polyclonal anti-OLIG2 (1:500, #AB9610, MilliporeSigma), rabbit polyclonal anti-NEUN (1:1000, #ABN78, MilliporeSigma), mouse monoclonal antiMASH1 (1:200, #556604, BD Biosciences), chicken polyclonal anti-RFP (1:500, #600-901-379, Rockland immunochemicals), rabbit polyclonal anti-H4K20ME3 (1:500, #pAb-057-050, Diagenode), rabbit polyclonal antiH3K9AC (1:500, #ab10812, Abcam).

    Article Title: Interstitial-fluid shear stresses induced by vertically oscillating head motion lower blood pressure in hypertensive rats and humans.
    Article Snippet: Shuhei Murase, Naoyoshi Sakitani, Takahiro Maekawa, Daisuke Yoshino , Kouji Takano, Ayumu Konno , Hirokazu Hirai , Taku Saito , Sakae Tanaka, Keisuke Shinohara, Takuya Kishi, Yuki Yoshikawa , Takamasa Sakai , Makoto Ayaori, Hirohiko Inanami, Koji Tomiyasu, Atsushi Takashima, Toru Ogata , Hirotsugu Tsuchimochi , Shinya Sato, Shigeyoshi Saito, Kohzoh Yoshino, Yuiko Matsuura , Kenichi Funamoto , Hiroki Ochi, Masahiro Shinohara, Motoshi Nagao & Yasuhiro Sawada

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: Compound Sakura Finetek Cat# 4583 Vectashield Mounting Medium with DAPI Vector Laboratories Cat# H-1200-10 Critical commercial assays Jo urn al Pr e-p roo f Phosphorylated Neurofilament H Human ELISA BioVendor Cat# RD191138300R Corticosterone EIA IDS Cat# AC-14F1 BCA Protein Assay Kit Pierce Cat# 23225 Deposited data Raw data This paper https://doi.org/10.3494 5/F52P4M Experimental models: Organisms/strains Sprague-Dawley Rat Charles River Laboratories RRID: MGI: 5651135 Software and algorithms Kinema Tracer System Kissei Comtec http://www.kinematrac er.com/ Odyssey Application Software Version 3.0 LI-COR Biosciences RRID: SCR_014579 NIS Elements Imaging Software Nikon Instruments RRID: SCR_014329; https://calm.ucsf.edu/h ardware-and-softwareimage-analysis ImageJ NIH RRID: SCR_003070 AutoQuant Media Cybernetics RRID: SCR_002465 MetaMorph Automation and Image Analysis Software Molecular Devices RRID: SCR_002368 Adobe Photoshop Adobe RRID: SCR_014199 SPSS Statistics version 26.0 IBM RRID: SCR_016479 Graphpad Prism Graphpad RRID: SCR_002798 Adobe Illustrator Adobe RRID: SCR_010279 Other Infinite Horizon Impactor Precision Systems and Instrumentation IH-0400; https://psiimpactors.co m/product/ih400/ Treadmill Muromachi Kikai MK-680; https://muromachi.com /en/archives/english/19 36/ Biological Amplifier Nihon Kohden NEC Biotop 6R12; https://doi.org/10.1371/ journal.pone.0171937 AD Converter Cambridge Electronic Design Limited Micro 1401; https://ced.co.uk/produ cts/microwaveformio Constant Current Stimulator Digitimer DS7A; https://www.digitimer.c om/product/humanneurophysiology/periph eral-stimulators/ds7ads7ah-hv-currentstimulator/ Jo urn al P e-p roo f Plate Reader Tecan GENios; https://www.tecan.com/ customer-news/geniosan-innovativedetection-device-thatdelivers-flexibility-tothe-molecular-biologylaboratory-2347 Infrared Imaging System LI-COR Biosciences Odyssey Infrared Imaging System; RRID: SCR_023765; https://doi.org/10.1038/ s41598-024-70096-0 Cryostat Leica Biosystems CM1950; RRID: SCR_018061; https://www.leicabiosys tems.com/us/histologyequipment/cryostats/lei ca-cm1950/ Inverted Microscope Nikon Instruments ECLIPSE Ti inverted microscope system; RRID: SCR_021242; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Spinning Disk Confocal Microscope Yokogawa Electric Corporation CSU-22; RRID: SCR_020907; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Jo urn al Pr e-p roo f

    Article Title: Atp13a5 Marker Reveals Pericyte Specification in the Mouse Central Nervous System
    Article Snippet: Rabbit polyclonal anti-NeuN , Millipore Sigma , ABN78.

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: For assessment of lesion pathology in the experimental animal model, the tissue sections of the thoracic spinal cord segments were incubated with Jo urn al Pr e-p ro f 58 diluted primary antibodies: rabbit polyclonal anti-NeuN (1:500; Millipore; Cat# ABN78; RRID: AB_10807945), mouse monoclonal anti-GFAP (1:500; Millipore; Cat# MAB360; RRID: AB_11212597), rabbit monoclonal anti-Vimentin (1:50; Cell Signaling Technology; Cat# 5741; RRID: AB_10695459), and rabbit polyclonal anti-GAP43 (1:500; Millipore; Cat# AB5220; RRID: AB_2107282).

    Article Title: SETD4 cells contribute to brain development and maintain adult stem cell reservoir for neurogenesis
    Article Snippet: The antibodies used in this study included mouse monoclonal anti-SETD4 (1:100, #sc-514060, Santa Cruz Biotechnology), rabbit polyclonal anti-GFAP (1:1000, #Z0334, Dako), goat polyclonal anti-GFAP (1:1000, #ab53554, Abcam), chicken polyclonal anti-NESTIN (1:1000, #NB100-1604, Novus Biologicals), mouse monoclonal anti-NESTIN (1:500, #MAB353, MilliporeSigma), rabbit polyclonal anti-SOX2 (1:200, #ab97959, Abcam), goat polyclonal anti-SOX2 (1:200, #AF2018, R&D Systems), rabbit monoclonal anti-KI67 (1:200, #ab16667, Abcam), mouse monoclonal antiPCNA (1:500, #ab29, Abcam), chicken polyclonal anti-GFP (1:1000, #ab13970, Abcam), rat monoclonal anti-BrdU (1:200, #ab6326, Abcam), goat polyclonal anti-DCX (1:500, #ab113435, Abcam), rabbit polyclonal anti-OLIG2 (1:500, #AB9610, MilliporeSigma), rabbit polyclonal anti-NEUN (1:1000, #ABN78, MilliporeSigma), mouse monoclonal antiMASH1 (1:200, #556604, BD Biosciences), chicken polyclonal anti-RFP (1:500, #600-901-379, Rockland immunochemicals), rabbit polyclonal anti-H4K20ME3 (1:500, #pAb-057-050, Diagenode), rabbit polyclonal antiH3K9AC (1:500, #ab10812, Abcam).

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: Rabbit Polyclonal Anti-NeuN , Millipore , Cat# ABN78; RRID: AB_10807945.

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: For assessment of lesion pathology in the experimental animal model, the tissue sections of the thoracic spinal cord segments were incubated with diluted primary antibodies: rabbit polyclonal anti-NeuN (1:500; Millipore; Cat# ABN78; RRID: AB_10807945 ), mouse monoclonal anti-GFAP (1:500; Millipore; Cat# MAB360; RRID: AB_11212597 ), rabbit monoclonal anti-Vimentin (1:50; Cell Signaling Technology; Cat# 5741; RRID: AB_10695459 ), and rabbit polyclonal anti-GAP43 (1:500; Millipore; Cat# AB5220; RRID: AB_2107282 ).

    Plasmid Preparation:

    Article Title: SETD4 cells contribute to brain development and maintain adult stem cell reservoir for neurogenesis
    Article Snippet: The antibodies used in this study included mouse monoclonal anti-SETD4 (1:100, #sc-514060, Santa Cruz Biotechnology), rabbit polyclonal anti-GFAP (1:1000, #Z0334, Dako), goat polyclonal anti-GFAP (1:1000, #ab53554, Abcam), chicken polyclonal anti-NESTIN (1:1000, #NB100-1604, Novus Biologicals), mouse monoclonal anti-NESTIN (1:500, #MAB353, MilliporeSigma), rabbit polyclonal anti-SOX2 (1:200, #ab97959, Abcam), goat polyclonal anti-SOX2 (1:200, #AF2018, R&D Systems), rabbit monoclonal anti-KI67 (1:200, #ab16667, Abcam), mouse monoclonal antiPCNA (1:500, #ab29, Abcam), chicken polyclonal anti-GFP (1:1000, #ab13970, Abcam), rat monoclonal anti-BrdU (1:200, #ab6326, Abcam), goat polyclonal anti-DCX (1:500, #ab113435, Abcam), rabbit polyclonal anti-OLIG2 (1:500, #AB9610, MilliporeSigma), rabbit polyclonal anti-NEUN (1:1000, #ABN78, MilliporeSigma), mouse monoclonal antiMASH1 (1:200, #556604, BD Biosciences), chicken polyclonal anti-RFP (1:500, #600-901-379, Rockland immunochemicals), rabbit polyclonal anti-H4K20ME3 (1:500, #pAb-057-050, Diagenode), rabbit polyclonal antiH3K9AC (1:500, #ab10812, Abcam).

    Article Title: Interstitial-fluid shear stresses induced by vertically oscillating head motion lower blood pressure in hypertensive rats and humans.
    Article Snippet: Shuhei Murase, Naoyoshi Sakitani, Takahiro Maekawa, Daisuke Yoshino , Kouji Takano, Ayumu Konno , Hirokazu Hirai , Taku Saito , Sakae Tanaka, Keisuke Shinohara, Takuya Kishi, Yuki Yoshikawa , Takamasa Sakai , Makoto Ayaori, Hirohiko Inanami, Koji Tomiyasu, Atsushi Takashima, Toru Ogata , Hirotsugu Tsuchimochi , Shinya Sato, Shigeyoshi Saito, Kohzoh Yoshino, Yuiko Matsuura , Kenichi Funamoto , Hiroki Ochi, Masahiro Shinohara, Motoshi Nagao & Yasuhiro Sawada

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: Compound Sakura Finetek Cat# 4583 Vectashield Mounting Medium with DAPI Vector Laboratories Cat# H-1200-10 Critical commercial assays Jo urn al Pr e-p roo f Phosphorylated Neurofilament H Human ELISA BioVendor Cat# RD191138300R Corticosterone EIA IDS Cat# AC-14F1 BCA Protein Assay Kit Pierce Cat# 23225 Deposited data Raw data This paper https://doi.org/10.3494 5/F52P4M Experimental models: Organisms/strains Sprague-Dawley Rat Charles River Laboratories RRID: MGI: 5651135 Software and algorithms Kinema Tracer System Kissei Comtec http://www.kinematrac er.com/ Odyssey Application Software Version 3.0 LI-COR Biosciences RRID: SCR_014579 NIS Elements Imaging Software Nikon Instruments RRID: SCR_014329; https://calm.ucsf.edu/h ardware-and-softwareimage-analysis ImageJ NIH RRID: SCR_003070 AutoQuant Media Cybernetics RRID: SCR_002465 MetaMorph Automation and Image Analysis Software Molecular Devices RRID: SCR_002368 Adobe Photoshop Adobe RRID: SCR_014199 SPSS Statistics version 26.0 IBM RRID: SCR_016479 Graphpad Prism Graphpad RRID: SCR_002798 Adobe Illustrator Adobe RRID: SCR_010279 Other Infinite Horizon Impactor Precision Systems and Instrumentation IH-0400; https://psiimpactors.co m/product/ih400/ Treadmill Muromachi Kikai MK-680; https://muromachi.com /en/archives/english/19 36/ Biological Amplifier Nihon Kohden NEC Biotop 6R12; https://doi.org/10.1371/ journal.pone.0171937 AD Converter Cambridge Electronic Design Limited Micro 1401; https://ced.co.uk/produ cts/microwaveformio Constant Current Stimulator Digitimer DS7A; https://www.digitimer.c om/product/humanneurophysiology/periph eral-stimulators/ds7ads7ah-hv-currentstimulator/ Jo urn al P e-p roo f Plate Reader Tecan GENios; https://www.tecan.com/ customer-news/geniosan-innovativedetection-device-thatdelivers-flexibility-tothe-molecular-biologylaboratory-2347 Infrared Imaging System LI-COR Biosciences Odyssey Infrared Imaging System; RRID: SCR_023765; https://doi.org/10.1038/ s41598-024-70096-0 Cryostat Leica Biosystems CM1950; RRID: SCR_018061; https://www.leicabiosys tems.com/us/histologyequipment/cryostats/lei ca-cm1950/ Inverted Microscope Nikon Instruments ECLIPSE Ti inverted microscope system; RRID: SCR_021242; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Spinning Disk Confocal Microscope Yokogawa Electric Corporation CSU-22; RRID: SCR_020907; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Jo urn al Pr e-p roo f

    Article Title: Atp13a5 Marker Reveals Pericyte Specification in the Mouse Central Nervous System
    Article Snippet: Rabbit polyclonal anti-NeuN , Millipore Sigma , ABN78.

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: For assessment of lesion pathology in the experimental animal model, the tissue sections of the thoracic spinal cord segments were incubated with Jo urn al Pr e-p ro f 58 diluted primary antibodies: rabbit polyclonal anti-NeuN (1:500; Millipore; Cat# ABN78; RRID: AB_10807945), mouse monoclonal anti-GFAP (1:500; Millipore; Cat# MAB360; RRID: AB_11212597), rabbit monoclonal anti-Vimentin (1:50; Cell Signaling Technology; Cat# 5741; RRID: AB_10695459), and rabbit polyclonal anti-GAP43 (1:500; Millipore; Cat# AB5220; RRID: AB_2107282).

    Article Title: SETD4 cells contribute to brain development and maintain adult stem cell reservoir for neurogenesis
    Article Snippet: The antibodies used in this study included mouse monoclonal anti-SETD4 (1:100, #sc-514060, Santa Cruz Biotechnology), rabbit polyclonal anti-GFAP (1:1000, #Z0334, Dako), goat polyclonal anti-GFAP (1:1000, #ab53554, Abcam), chicken polyclonal anti-NESTIN (1:1000, #NB100-1604, Novus Biologicals), mouse monoclonal anti-NESTIN (1:500, #MAB353, MilliporeSigma), rabbit polyclonal anti-SOX2 (1:200, #ab97959, Abcam), goat polyclonal anti-SOX2 (1:200, #AF2018, R&D Systems), rabbit monoclonal anti-KI67 (1:200, #ab16667, Abcam), mouse monoclonal antiPCNA (1:500, #ab29, Abcam), chicken polyclonal anti-GFP (1:1000, #ab13970, Abcam), rat monoclonal anti-BrdU (1:200, #ab6326, Abcam), goat polyclonal anti-DCX (1:500, #ab113435, Abcam), rabbit polyclonal anti-OLIG2 (1:500, #AB9610, MilliporeSigma), rabbit polyclonal anti-NEUN (1:1000, #ABN78, MilliporeSigma), mouse monoclonal antiMASH1 (1:200, #556604, BD Biosciences), chicken polyclonal anti-RFP (1:500, #600-901-379, Rockland immunochemicals), rabbit polyclonal anti-H4K20ME3 (1:500, #pAb-057-050, Diagenode), rabbit polyclonal antiH3K9AC (1:500, #ab10812, Abcam).

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: Rabbit Polyclonal Anti-NeuN , Millipore , Cat# ABN78; RRID: AB_10807945.

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: For assessment of lesion pathology in the experimental animal model, the tissue sections of the thoracic spinal cord segments were incubated with diluted primary antibodies: rabbit polyclonal anti-NeuN (1:500; Millipore; Cat# ABN78; RRID: AB_10807945 ), mouse monoclonal anti-GFAP (1:500; Millipore; Cat# MAB360; RRID: AB_11212597 ), rabbit monoclonal anti-Vimentin (1:50; Cell Signaling Technology; Cat# 5741; RRID: AB_10695459 ), and rabbit polyclonal anti-GAP43 (1:500; Millipore; Cat# AB5220; RRID: AB_2107282 ).

    Enzyme-linked Immunosorbent Assay:

    Article Title: SETD4 cells contribute to brain development and maintain adult stem cell reservoir for neurogenesis
    Article Snippet: The antibodies used in this study included mouse monoclonal anti-SETD4 (1:100, #sc-514060, Santa Cruz Biotechnology), rabbit polyclonal anti-GFAP (1:1000, #Z0334, Dako), goat polyclonal anti-GFAP (1:1000, #ab53554, Abcam), chicken polyclonal anti-NESTIN (1:1000, #NB100-1604, Novus Biologicals), mouse monoclonal anti-NESTIN (1:500, #MAB353, MilliporeSigma), rabbit polyclonal anti-SOX2 (1:200, #ab97959, Abcam), goat polyclonal anti-SOX2 (1:200, #AF2018, R&D Systems), rabbit monoclonal anti-KI67 (1:200, #ab16667, Abcam), mouse monoclonal antiPCNA (1:500, #ab29, Abcam), chicken polyclonal anti-GFP (1:1000, #ab13970, Abcam), rat monoclonal anti-BrdU (1:200, #ab6326, Abcam), goat polyclonal anti-DCX (1:500, #ab113435, Abcam), rabbit polyclonal anti-OLIG2 (1:500, #AB9610, MilliporeSigma), rabbit polyclonal anti-NEUN (1:1000, #ABN78, MilliporeSigma), mouse monoclonal antiMASH1 (1:200, #556604, BD Biosciences), chicken polyclonal anti-RFP (1:500, #600-901-379, Rockland immunochemicals), rabbit polyclonal anti-H4K20ME3 (1:500, #pAb-057-050, Diagenode), rabbit polyclonal antiH3K9AC (1:500, #ab10812, Abcam).

    Article Title: Interstitial-fluid shear stresses induced by vertically oscillating head motion lower blood pressure in hypertensive rats and humans.
    Article Snippet: Shuhei Murase, Naoyoshi Sakitani, Takahiro Maekawa, Daisuke Yoshino , Kouji Takano, Ayumu Konno , Hirokazu Hirai , Taku Saito , Sakae Tanaka, Keisuke Shinohara, Takuya Kishi, Yuki Yoshikawa , Takamasa Sakai , Makoto Ayaori, Hirohiko Inanami, Koji Tomiyasu, Atsushi Takashima, Toru Ogata , Hirotsugu Tsuchimochi , Shinya Sato, Shigeyoshi Saito, Kohzoh Yoshino, Yuiko Matsuura , Kenichi Funamoto , Hiroki Ochi, Masahiro Shinohara, Motoshi Nagao & Yasuhiro Sawada

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: Compound Sakura Finetek Cat# 4583 Vectashield Mounting Medium with DAPI Vector Laboratories Cat# H-1200-10 Critical commercial assays Jo urn al Pr e-p roo f Phosphorylated Neurofilament H Human ELISA BioVendor Cat# RD191138300R Corticosterone EIA IDS Cat# AC-14F1 BCA Protein Assay Kit Pierce Cat# 23225 Deposited data Raw data This paper https://doi.org/10.3494 5/F52P4M Experimental models: Organisms/strains Sprague-Dawley Rat Charles River Laboratories RRID: MGI: 5651135 Software and algorithms Kinema Tracer System Kissei Comtec http://www.kinematrac er.com/ Odyssey Application Software Version 3.0 LI-COR Biosciences RRID: SCR_014579 NIS Elements Imaging Software Nikon Instruments RRID: SCR_014329; https://calm.ucsf.edu/h ardware-and-softwareimage-analysis ImageJ NIH RRID: SCR_003070 AutoQuant Media Cybernetics RRID: SCR_002465 MetaMorph Automation and Image Analysis Software Molecular Devices RRID: SCR_002368 Adobe Photoshop Adobe RRID: SCR_014199 SPSS Statistics version 26.0 IBM RRID: SCR_016479 Graphpad Prism Graphpad RRID: SCR_002798 Adobe Illustrator Adobe RRID: SCR_010279 Other Infinite Horizon Impactor Precision Systems and Instrumentation IH-0400; https://psiimpactors.co m/product/ih400/ Treadmill Muromachi Kikai MK-680; https://muromachi.com /en/archives/english/19 36/ Biological Amplifier Nihon Kohden NEC Biotop 6R12; https://doi.org/10.1371/ journal.pone.0171937 AD Converter Cambridge Electronic Design Limited Micro 1401; https://ced.co.uk/produ cts/microwaveformio Constant Current Stimulator Digitimer DS7A; https://www.digitimer.c om/product/humanneurophysiology/periph eral-stimulators/ds7ads7ah-hv-currentstimulator/ Jo urn al P e-p roo f Plate Reader Tecan GENios; https://www.tecan.com/ customer-news/geniosan-innovativedetection-device-thatdelivers-flexibility-tothe-molecular-biologylaboratory-2347 Infrared Imaging System LI-COR Biosciences Odyssey Infrared Imaging System; RRID: SCR_023765; https://doi.org/10.1038/ s41598-024-70096-0 Cryostat Leica Biosystems CM1950; RRID: SCR_018061; https://www.leicabiosys tems.com/us/histologyequipment/cryostats/lei ca-cm1950/ Inverted Microscope Nikon Instruments ECLIPSE Ti inverted microscope system; RRID: SCR_021242; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Spinning Disk Confocal Microscope Yokogawa Electric Corporation CSU-22; RRID: SCR_020907; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Jo urn al Pr e-p roo f

    Article Title: Atp13a5 Marker Reveals Pericyte Specification in the Mouse Central Nervous System
    Article Snippet: Rabbit polyclonal anti-NeuN , Millipore Sigma , ABN78.

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: For assessment of lesion pathology in the experimental animal model, the tissue sections of the thoracic spinal cord segments were incubated with Jo urn al Pr e-p ro f 58 diluted primary antibodies: rabbit polyclonal anti-NeuN (1:500; Millipore; Cat# ABN78; RRID: AB_10807945), mouse monoclonal anti-GFAP (1:500; Millipore; Cat# MAB360; RRID: AB_11212597), rabbit monoclonal anti-Vimentin (1:50; Cell Signaling Technology; Cat# 5741; RRID: AB_10695459), and rabbit polyclonal anti-GAP43 (1:500; Millipore; Cat# AB5220; RRID: AB_2107282).

    Article Title: SETD4 cells contribute to brain development and maintain adult stem cell reservoir for neurogenesis
    Article Snippet: The antibodies used in this study included mouse monoclonal anti-SETD4 (1:100, #sc-514060, Santa Cruz Biotechnology), rabbit polyclonal anti-GFAP (1:1000, #Z0334, Dako), goat polyclonal anti-GFAP (1:1000, #ab53554, Abcam), chicken polyclonal anti-NESTIN (1:1000, #NB100-1604, Novus Biologicals), mouse monoclonal anti-NESTIN (1:500, #MAB353, MilliporeSigma), rabbit polyclonal anti-SOX2 (1:200, #ab97959, Abcam), goat polyclonal anti-SOX2 (1:200, #AF2018, R&D Systems), rabbit monoclonal anti-KI67 (1:200, #ab16667, Abcam), mouse monoclonal antiPCNA (1:500, #ab29, Abcam), chicken polyclonal anti-GFP (1:1000, #ab13970, Abcam), rat monoclonal anti-BrdU (1:200, #ab6326, Abcam), goat polyclonal anti-DCX (1:500, #ab113435, Abcam), rabbit polyclonal anti-OLIG2 (1:500, #AB9610, MilliporeSigma), rabbit polyclonal anti-NEUN (1:1000, #ABN78, MilliporeSigma), mouse monoclonal antiMASH1 (1:200, #556604, BD Biosciences), chicken polyclonal anti-RFP (1:500, #600-901-379, Rockland immunochemicals), rabbit polyclonal anti-H4K20ME3 (1:500, #pAb-057-050, Diagenode), rabbit polyclonal antiH3K9AC (1:500, #ab10812, Abcam).

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: Rabbit Polyclonal Anti-NeuN , Millipore , Cat# ABN78; RRID: AB_10807945.

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: For assessment of lesion pathology in the experimental animal model, the tissue sections of the thoracic spinal cord segments were incubated with diluted primary antibodies: rabbit polyclonal anti-NeuN (1:500; Millipore; Cat# ABN78; RRID: AB_10807945 ), mouse monoclonal anti-GFAP (1:500; Millipore; Cat# MAB360; RRID: AB_11212597 ), rabbit monoclonal anti-Vimentin (1:50; Cell Signaling Technology; Cat# 5741; RRID: AB_10695459 ), and rabbit polyclonal anti-GAP43 (1:500; Millipore; Cat# AB5220; RRID: AB_2107282 ).

    Bicinchoninic Acid Protein Assay:

    Article Title: SETD4 cells contribute to brain development and maintain adult stem cell reservoir for neurogenesis
    Article Snippet: The antibodies used in this study included mouse monoclonal anti-SETD4 (1:100, #sc-514060, Santa Cruz Biotechnology), rabbit polyclonal anti-GFAP (1:1000, #Z0334, Dako), goat polyclonal anti-GFAP (1:1000, #ab53554, Abcam), chicken polyclonal anti-NESTIN (1:1000, #NB100-1604, Novus Biologicals), mouse monoclonal anti-NESTIN (1:500, #MAB353, MilliporeSigma), rabbit polyclonal anti-SOX2 (1:200, #ab97959, Abcam), goat polyclonal anti-SOX2 (1:200, #AF2018, R&D Systems), rabbit monoclonal anti-KI67 (1:200, #ab16667, Abcam), mouse monoclonal antiPCNA (1:500, #ab29, Abcam), chicken polyclonal anti-GFP (1:1000, #ab13970, Abcam), rat monoclonal anti-BrdU (1:200, #ab6326, Abcam), goat polyclonal anti-DCX (1:500, #ab113435, Abcam), rabbit polyclonal anti-OLIG2 (1:500, #AB9610, MilliporeSigma), rabbit polyclonal anti-NEUN (1:1000, #ABN78, MilliporeSigma), mouse monoclonal antiMASH1 (1:200, #556604, BD Biosciences), chicken polyclonal anti-RFP (1:500, #600-901-379, Rockland immunochemicals), rabbit polyclonal anti-H4K20ME3 (1:500, #pAb-057-050, Diagenode), rabbit polyclonal antiH3K9AC (1:500, #ab10812, Abcam).

    Article Title: Interstitial-fluid shear stresses induced by vertically oscillating head motion lower blood pressure in hypertensive rats and humans.
    Article Snippet: Shuhei Murase, Naoyoshi Sakitani, Takahiro Maekawa, Daisuke Yoshino , Kouji Takano, Ayumu Konno , Hirokazu Hirai , Taku Saito , Sakae Tanaka, Keisuke Shinohara, Takuya Kishi, Yuki Yoshikawa , Takamasa Sakai , Makoto Ayaori, Hirohiko Inanami, Koji Tomiyasu, Atsushi Takashima, Toru Ogata , Hirotsugu Tsuchimochi , Shinya Sato, Shigeyoshi Saito, Kohzoh Yoshino, Yuiko Matsuura , Kenichi Funamoto , Hiroki Ochi, Masahiro Shinohara, Motoshi Nagao & Yasuhiro Sawada

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: Compound Sakura Finetek Cat# 4583 Vectashield Mounting Medium with DAPI Vector Laboratories Cat# H-1200-10 Critical commercial assays Jo urn al Pr e-p roo f Phosphorylated Neurofilament H Human ELISA BioVendor Cat# RD191138300R Corticosterone EIA IDS Cat# AC-14F1 BCA Protein Assay Kit Pierce Cat# 23225 Deposited data Raw data This paper https://doi.org/10.3494 5/F52P4M Experimental models: Organisms/strains Sprague-Dawley Rat Charles River Laboratories RRID: MGI: 5651135 Software and algorithms Kinema Tracer System Kissei Comtec http://www.kinematrac er.com/ Odyssey Application Software Version 3.0 LI-COR Biosciences RRID: SCR_014579 NIS Elements Imaging Software Nikon Instruments RRID: SCR_014329; https://calm.ucsf.edu/h ardware-and-softwareimage-analysis ImageJ NIH RRID: SCR_003070 AutoQuant Media Cybernetics RRID: SCR_002465 MetaMorph Automation and Image Analysis Software Molecular Devices RRID: SCR_002368 Adobe Photoshop Adobe RRID: SCR_014199 SPSS Statistics version 26.0 IBM RRID: SCR_016479 Graphpad Prism Graphpad RRID: SCR_002798 Adobe Illustrator Adobe RRID: SCR_010279 Other Infinite Horizon Impactor Precision Systems and Instrumentation IH-0400; https://psiimpactors.co m/product/ih400/ Treadmill Muromachi Kikai MK-680; https://muromachi.com /en/archives/english/19 36/ Biological Amplifier Nihon Kohden NEC Biotop 6R12; https://doi.org/10.1371/ journal.pone.0171937 AD Converter Cambridge Electronic Design Limited Micro 1401; https://ced.co.uk/produ cts/microwaveformio Constant Current Stimulator Digitimer DS7A; https://www.digitimer.c om/product/humanneurophysiology/periph eral-stimulators/ds7ads7ah-hv-currentstimulator/ Jo urn al P e-p roo f Plate Reader Tecan GENios; https://www.tecan.com/ customer-news/geniosan-innovativedetection-device-thatdelivers-flexibility-tothe-molecular-biologylaboratory-2347 Infrared Imaging System LI-COR Biosciences Odyssey Infrared Imaging System; RRID: SCR_023765; https://doi.org/10.1038/ s41598-024-70096-0 Cryostat Leica Biosystems CM1950; RRID: SCR_018061; https://www.leicabiosys tems.com/us/histologyequipment/cryostats/lei ca-cm1950/ Inverted Microscope Nikon Instruments ECLIPSE Ti inverted microscope system; RRID: SCR_021242; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Spinning Disk Confocal Microscope Yokogawa Electric Corporation CSU-22; RRID: SCR_020907; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Jo urn al Pr e-p roo f

    Article Title: Atp13a5 Marker Reveals Pericyte Specification in the Mouse Central Nervous System
    Article Snippet: Rabbit polyclonal anti-NeuN , Millipore Sigma , ABN78.

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: For assessment of lesion pathology in the experimental animal model, the tissue sections of the thoracic spinal cord segments were incubated with Jo urn al Pr e-p ro f 58 diluted primary antibodies: rabbit polyclonal anti-NeuN (1:500; Millipore; Cat# ABN78; RRID: AB_10807945), mouse monoclonal anti-GFAP (1:500; Millipore; Cat# MAB360; RRID: AB_11212597), rabbit monoclonal anti-Vimentin (1:50; Cell Signaling Technology; Cat# 5741; RRID: AB_10695459), and rabbit polyclonal anti-GAP43 (1:500; Millipore; Cat# AB5220; RRID: AB_2107282).

    Article Title: SETD4 cells contribute to brain development and maintain adult stem cell reservoir for neurogenesis
    Article Snippet: The antibodies used in this study included mouse monoclonal anti-SETD4 (1:100, #sc-514060, Santa Cruz Biotechnology), rabbit polyclonal anti-GFAP (1:1000, #Z0334, Dako), goat polyclonal anti-GFAP (1:1000, #ab53554, Abcam), chicken polyclonal anti-NESTIN (1:1000, #NB100-1604, Novus Biologicals), mouse monoclonal anti-NESTIN (1:500, #MAB353, MilliporeSigma), rabbit polyclonal anti-SOX2 (1:200, #ab97959, Abcam), goat polyclonal anti-SOX2 (1:200, #AF2018, R&D Systems), rabbit monoclonal anti-KI67 (1:200, #ab16667, Abcam), mouse monoclonal antiPCNA (1:500, #ab29, Abcam), chicken polyclonal anti-GFP (1:1000, #ab13970, Abcam), rat monoclonal anti-BrdU (1:200, #ab6326, Abcam), goat polyclonal anti-DCX (1:500, #ab113435, Abcam), rabbit polyclonal anti-OLIG2 (1:500, #AB9610, MilliporeSigma), rabbit polyclonal anti-NEUN (1:1000, #ABN78, MilliporeSigma), mouse monoclonal antiMASH1 (1:200, #556604, BD Biosciences), chicken polyclonal anti-RFP (1:500, #600-901-379, Rockland immunochemicals), rabbit polyclonal anti-H4K20ME3 (1:500, #pAb-057-050, Diagenode), rabbit polyclonal antiH3K9AC (1:500, #ab10812, Abcam).

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: Rabbit Polyclonal Anti-NeuN , Millipore , Cat# ABN78; RRID: AB_10807945.

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: For assessment of lesion pathology in the experimental animal model, the tissue sections of the thoracic spinal cord segments were incubated with diluted primary antibodies: rabbit polyclonal anti-NeuN (1:500; Millipore; Cat# ABN78; RRID: AB_10807945 ), mouse monoclonal anti-GFAP (1:500; Millipore; Cat# MAB360; RRID: AB_11212597 ), rabbit monoclonal anti-Vimentin (1:50; Cell Signaling Technology; Cat# 5741; RRID: AB_10695459 ), and rabbit polyclonal anti-GAP43 (1:500; Millipore; Cat# AB5220; RRID: AB_2107282 ).

    Software:

    Article Title: SETD4 cells contribute to brain development and maintain adult stem cell reservoir for neurogenesis
    Article Snippet: The antibodies used in this study included mouse monoclonal anti-SETD4 (1:100, #sc-514060, Santa Cruz Biotechnology), rabbit polyclonal anti-GFAP (1:1000, #Z0334, Dako), goat polyclonal anti-GFAP (1:1000, #ab53554, Abcam), chicken polyclonal anti-NESTIN (1:1000, #NB100-1604, Novus Biologicals), mouse monoclonal anti-NESTIN (1:500, #MAB353, MilliporeSigma), rabbit polyclonal anti-SOX2 (1:200, #ab97959, Abcam), goat polyclonal anti-SOX2 (1:200, #AF2018, R&D Systems), rabbit monoclonal anti-KI67 (1:200, #ab16667, Abcam), mouse monoclonal antiPCNA (1:500, #ab29, Abcam), chicken polyclonal anti-GFP (1:1000, #ab13970, Abcam), rat monoclonal anti-BrdU (1:200, #ab6326, Abcam), goat polyclonal anti-DCX (1:500, #ab113435, Abcam), rabbit polyclonal anti-OLIG2 (1:500, #AB9610, MilliporeSigma), rabbit polyclonal anti-NEUN (1:1000, #ABN78, MilliporeSigma), mouse monoclonal antiMASH1 (1:200, #556604, BD Biosciences), chicken polyclonal anti-RFP (1:500, #600-901-379, Rockland immunochemicals), rabbit polyclonal anti-H4K20ME3 (1:500, #pAb-057-050, Diagenode), rabbit polyclonal antiH3K9AC (1:500, #ab10812, Abcam).

    Article Title: Interstitial-fluid shear stresses induced by vertically oscillating head motion lower blood pressure in hypertensive rats and humans.
    Article Snippet: Shuhei Murase, Naoyoshi Sakitani, Takahiro Maekawa, Daisuke Yoshino , Kouji Takano, Ayumu Konno , Hirokazu Hirai , Taku Saito , Sakae Tanaka, Keisuke Shinohara, Takuya Kishi, Yuki Yoshikawa , Takamasa Sakai , Makoto Ayaori, Hirohiko Inanami, Koji Tomiyasu, Atsushi Takashima, Toru Ogata , Hirotsugu Tsuchimochi , Shinya Sato, Shigeyoshi Saito, Kohzoh Yoshino, Yuiko Matsuura , Kenichi Funamoto , Hiroki Ochi, Masahiro Shinohara, Motoshi Nagao & Yasuhiro Sawada

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: Compound Sakura Finetek Cat# 4583 Vectashield Mounting Medium with DAPI Vector Laboratories Cat# H-1200-10 Critical commercial assays Jo urn al Pr e-p roo f Phosphorylated Neurofilament H Human ELISA BioVendor Cat# RD191138300R Corticosterone EIA IDS Cat# AC-14F1 BCA Protein Assay Kit Pierce Cat# 23225 Deposited data Raw data This paper https://doi.org/10.3494 5/F52P4M Experimental models: Organisms/strains Sprague-Dawley Rat Charles River Laboratories RRID: MGI: 5651135 Software and algorithms Kinema Tracer System Kissei Comtec http://www.kinematrac er.com/ Odyssey Application Software Version 3.0 LI-COR Biosciences RRID: SCR_014579 NIS Elements Imaging Software Nikon Instruments RRID: SCR_014329; https://calm.ucsf.edu/h ardware-and-softwareimage-analysis ImageJ NIH RRID: SCR_003070 AutoQuant Media Cybernetics RRID: SCR_002465 MetaMorph Automation and Image Analysis Software Molecular Devices RRID: SCR_002368 Adobe Photoshop Adobe RRID: SCR_014199 SPSS Statistics version 26.0 IBM RRID: SCR_016479 Graphpad Prism Graphpad RRID: SCR_002798 Adobe Illustrator Adobe RRID: SCR_010279 Other Infinite Horizon Impactor Precision Systems and Instrumentation IH-0400; https://psiimpactors.co m/product/ih400/ Treadmill Muromachi Kikai MK-680; https://muromachi.com /en/archives/english/19 36/ Biological Amplifier Nihon Kohden NEC Biotop 6R12; https://doi.org/10.1371/ journal.pone.0171937 AD Converter Cambridge Electronic Design Limited Micro 1401; https://ced.co.uk/produ cts/microwaveformio Constant Current Stimulator Digitimer DS7A; https://www.digitimer.c om/product/humanneurophysiology/periph eral-stimulators/ds7ads7ah-hv-currentstimulator/ Jo urn al P e-p roo f Plate Reader Tecan GENios; https://www.tecan.com/ customer-news/geniosan-innovativedetection-device-thatdelivers-flexibility-tothe-molecular-biologylaboratory-2347 Infrared Imaging System LI-COR Biosciences Odyssey Infrared Imaging System; RRID: SCR_023765; https://doi.org/10.1038/ s41598-024-70096-0 Cryostat Leica Biosystems CM1950; RRID: SCR_018061; https://www.leicabiosys tems.com/us/histologyequipment/cryostats/lei ca-cm1950/ Inverted Microscope Nikon Instruments ECLIPSE Ti inverted microscope system; RRID: SCR_021242; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Spinning Disk Confocal Microscope Yokogawa Electric Corporation CSU-22; RRID: SCR_020907; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Jo urn al Pr e-p roo f

    Article Title: Atp13a5 Marker Reveals Pericyte Specification in the Mouse Central Nervous System
    Article Snippet: Rabbit polyclonal anti-NeuN , Millipore Sigma , ABN78.

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: For assessment of lesion pathology in the experimental animal model, the tissue sections of the thoracic spinal cord segments were incubated with Jo urn al Pr e-p ro f 58 diluted primary antibodies: rabbit polyclonal anti-NeuN (1:500; Millipore; Cat# ABN78; RRID: AB_10807945), mouse monoclonal anti-GFAP (1:500; Millipore; Cat# MAB360; RRID: AB_11212597), rabbit monoclonal anti-Vimentin (1:50; Cell Signaling Technology; Cat# 5741; RRID: AB_10695459), and rabbit polyclonal anti-GAP43 (1:500; Millipore; Cat# AB5220; RRID: AB_2107282).

    Article Title: SETD4 cells contribute to brain development and maintain adult stem cell reservoir for neurogenesis
    Article Snippet: The antibodies used in this study included mouse monoclonal anti-SETD4 (1:100, #sc-514060, Santa Cruz Biotechnology), rabbit polyclonal anti-GFAP (1:1000, #Z0334, Dako), goat polyclonal anti-GFAP (1:1000, #ab53554, Abcam), chicken polyclonal anti-NESTIN (1:1000, #NB100-1604, Novus Biologicals), mouse monoclonal anti-NESTIN (1:500, #MAB353, MilliporeSigma), rabbit polyclonal anti-SOX2 (1:200, #ab97959, Abcam), goat polyclonal anti-SOX2 (1:200, #AF2018, R&D Systems), rabbit monoclonal anti-KI67 (1:200, #ab16667, Abcam), mouse monoclonal antiPCNA (1:500, #ab29, Abcam), chicken polyclonal anti-GFP (1:1000, #ab13970, Abcam), rat monoclonal anti-BrdU (1:200, #ab6326, Abcam), goat polyclonal anti-DCX (1:500, #ab113435, Abcam), rabbit polyclonal anti-OLIG2 (1:500, #AB9610, MilliporeSigma), rabbit polyclonal anti-NEUN (1:1000, #ABN78, MilliporeSigma), mouse monoclonal antiMASH1 (1:200, #556604, BD Biosciences), chicken polyclonal anti-RFP (1:500, #600-901-379, Rockland immunochemicals), rabbit polyclonal anti-H4K20ME3 (1:500, #pAb-057-050, Diagenode), rabbit polyclonal antiH3K9AC (1:500, #ab10812, Abcam).

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: Rabbit Polyclonal Anti-NeuN , Millipore , Cat# ABN78; RRID: AB_10807945.

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: For assessment of lesion pathology in the experimental animal model, the tissue sections of the thoracic spinal cord segments were incubated with diluted primary antibodies: rabbit polyclonal anti-NeuN (1:500; Millipore; Cat# ABN78; RRID: AB_10807945 ), mouse monoclonal anti-GFAP (1:500; Millipore; Cat# MAB360; RRID: AB_11212597 ), rabbit monoclonal anti-Vimentin (1:50; Cell Signaling Technology; Cat# 5741; RRID: AB_10695459 ), and rabbit polyclonal anti-GAP43 (1:500; Millipore; Cat# AB5220; RRID: AB_2107282 ).

    Imaging:

    Article Title: SETD4 cells contribute to brain development and maintain adult stem cell reservoir for neurogenesis
    Article Snippet: The antibodies used in this study included mouse monoclonal anti-SETD4 (1:100, #sc-514060, Santa Cruz Biotechnology), rabbit polyclonal anti-GFAP (1:1000, #Z0334, Dako), goat polyclonal anti-GFAP (1:1000, #ab53554, Abcam), chicken polyclonal anti-NESTIN (1:1000, #NB100-1604, Novus Biologicals), mouse monoclonal anti-NESTIN (1:500, #MAB353, MilliporeSigma), rabbit polyclonal anti-SOX2 (1:200, #ab97959, Abcam), goat polyclonal anti-SOX2 (1:200, #AF2018, R&D Systems), rabbit monoclonal anti-KI67 (1:200, #ab16667, Abcam), mouse monoclonal antiPCNA (1:500, #ab29, Abcam), chicken polyclonal anti-GFP (1:1000, #ab13970, Abcam), rat monoclonal anti-BrdU (1:200, #ab6326, Abcam), goat polyclonal anti-DCX (1:500, #ab113435, Abcam), rabbit polyclonal anti-OLIG2 (1:500, #AB9610, MilliporeSigma), rabbit polyclonal anti-NEUN (1:1000, #ABN78, MilliporeSigma), mouse monoclonal antiMASH1 (1:200, #556604, BD Biosciences), chicken polyclonal anti-RFP (1:500, #600-901-379, Rockland immunochemicals), rabbit polyclonal anti-H4K20ME3 (1:500, #pAb-057-050, Diagenode), rabbit polyclonal antiH3K9AC (1:500, #ab10812, Abcam).

    Article Title: Interstitial-fluid shear stresses induced by vertically oscillating head motion lower blood pressure in hypertensive rats and humans.
    Article Snippet: Shuhei Murase, Naoyoshi Sakitani, Takahiro Maekawa, Daisuke Yoshino , Kouji Takano, Ayumu Konno , Hirokazu Hirai , Taku Saito , Sakae Tanaka, Keisuke Shinohara, Takuya Kishi, Yuki Yoshikawa , Takamasa Sakai , Makoto Ayaori, Hirohiko Inanami, Koji Tomiyasu, Atsushi Takashima, Toru Ogata , Hirotsugu Tsuchimochi , Shinya Sato, Shigeyoshi Saito, Kohzoh Yoshino, Yuiko Matsuura , Kenichi Funamoto , Hiroki Ochi, Masahiro Shinohara, Motoshi Nagao & Yasuhiro Sawada

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: Compound Sakura Finetek Cat# 4583 Vectashield Mounting Medium with DAPI Vector Laboratories Cat# H-1200-10 Critical commercial assays Jo urn al Pr e-p roo f Phosphorylated Neurofilament H Human ELISA BioVendor Cat# RD191138300R Corticosterone EIA IDS Cat# AC-14F1 BCA Protein Assay Kit Pierce Cat# 23225 Deposited data Raw data This paper https://doi.org/10.3494 5/F52P4M Experimental models: Organisms/strains Sprague-Dawley Rat Charles River Laboratories RRID: MGI: 5651135 Software and algorithms Kinema Tracer System Kissei Comtec http://www.kinematrac er.com/ Odyssey Application Software Version 3.0 LI-COR Biosciences RRID: SCR_014579 NIS Elements Imaging Software Nikon Instruments RRID: SCR_014329; https://calm.ucsf.edu/h ardware-and-softwareimage-analysis ImageJ NIH RRID: SCR_003070 AutoQuant Media Cybernetics RRID: SCR_002465 MetaMorph Automation and Image Analysis Software Molecular Devices RRID: SCR_002368 Adobe Photoshop Adobe RRID: SCR_014199 SPSS Statistics version 26.0 IBM RRID: SCR_016479 Graphpad Prism Graphpad RRID: SCR_002798 Adobe Illustrator Adobe RRID: SCR_010279 Other Infinite Horizon Impactor Precision Systems and Instrumentation IH-0400; https://psiimpactors.co m/product/ih400/ Treadmill Muromachi Kikai MK-680; https://muromachi.com /en/archives/english/19 36/ Biological Amplifier Nihon Kohden NEC Biotop 6R12; https://doi.org/10.1371/ journal.pone.0171937 AD Converter Cambridge Electronic Design Limited Micro 1401; https://ced.co.uk/produ cts/microwaveformio Constant Current Stimulator Digitimer DS7A; https://www.digitimer.c om/product/humanneurophysiology/periph eral-stimulators/ds7ads7ah-hv-currentstimulator/ Jo urn al P e-p roo f Plate Reader Tecan GENios; https://www.tecan.com/ customer-news/geniosan-innovativedetection-device-thatdelivers-flexibility-tothe-molecular-biologylaboratory-2347 Infrared Imaging System LI-COR Biosciences Odyssey Infrared Imaging System; RRID: SCR_023765; https://doi.org/10.1038/ s41598-024-70096-0 Cryostat Leica Biosystems CM1950; RRID: SCR_018061; https://www.leicabiosys tems.com/us/histologyequipment/cryostats/lei ca-cm1950/ Inverted Microscope Nikon Instruments ECLIPSE Ti inverted microscope system; RRID: SCR_021242; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Spinning Disk Confocal Microscope Yokogawa Electric Corporation CSU-22; RRID: SCR_020907; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Jo urn al Pr e-p roo f

    Article Title: Atp13a5 Marker Reveals Pericyte Specification in the Mouse Central Nervous System
    Article Snippet: Rabbit polyclonal anti-NeuN , Millipore Sigma , ABN78.

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: For assessment of lesion pathology in the experimental animal model, the tissue sections of the thoracic spinal cord segments were incubated with Jo urn al Pr e-p ro f 58 diluted primary antibodies: rabbit polyclonal anti-NeuN (1:500; Millipore; Cat# ABN78; RRID: AB_10807945), mouse monoclonal anti-GFAP (1:500; Millipore; Cat# MAB360; RRID: AB_11212597), rabbit monoclonal anti-Vimentin (1:50; Cell Signaling Technology; Cat# 5741; RRID: AB_10695459), and rabbit polyclonal anti-GAP43 (1:500; Millipore; Cat# AB5220; RRID: AB_2107282).

    Article Title: SETD4 cells contribute to brain development and maintain adult stem cell reservoir for neurogenesis
    Article Snippet: The antibodies used in this study included mouse monoclonal anti-SETD4 (1:100, #sc-514060, Santa Cruz Biotechnology), rabbit polyclonal anti-GFAP (1:1000, #Z0334, Dako), goat polyclonal anti-GFAP (1:1000, #ab53554, Abcam), chicken polyclonal anti-NESTIN (1:1000, #NB100-1604, Novus Biologicals), mouse monoclonal anti-NESTIN (1:500, #MAB353, MilliporeSigma), rabbit polyclonal anti-SOX2 (1:200, #ab97959, Abcam), goat polyclonal anti-SOX2 (1:200, #AF2018, R&D Systems), rabbit monoclonal anti-KI67 (1:200, #ab16667, Abcam), mouse monoclonal antiPCNA (1:500, #ab29, Abcam), chicken polyclonal anti-GFP (1:1000, #ab13970, Abcam), rat monoclonal anti-BrdU (1:200, #ab6326, Abcam), goat polyclonal anti-DCX (1:500, #ab113435, Abcam), rabbit polyclonal anti-OLIG2 (1:500, #AB9610, MilliporeSigma), rabbit polyclonal anti-NEUN (1:1000, #ABN78, MilliporeSigma), mouse monoclonal antiMASH1 (1:200, #556604, BD Biosciences), chicken polyclonal anti-RFP (1:500, #600-901-379, Rockland immunochemicals), rabbit polyclonal anti-H4K20ME3 (1:500, #pAb-057-050, Diagenode), rabbit polyclonal antiH3K9AC (1:500, #ab10812, Abcam).

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: Rabbit Polyclonal Anti-NeuN , Millipore , Cat# ABN78; RRID: AB_10807945.

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: For assessment of lesion pathology in the experimental animal model, the tissue sections of the thoracic spinal cord segments were incubated with diluted primary antibodies: rabbit polyclonal anti-NeuN (1:500; Millipore; Cat# ABN78; RRID: AB_10807945 ), mouse monoclonal anti-GFAP (1:500; Millipore; Cat# MAB360; RRID: AB_11212597 ), rabbit monoclonal anti-Vimentin (1:50; Cell Signaling Technology; Cat# 5741; RRID: AB_10695459 ), and rabbit polyclonal anti-GAP43 (1:500; Millipore; Cat# AB5220; RRID: AB_2107282 ).

    Inverted Microscopy:

    Article Title: SETD4 cells contribute to brain development and maintain adult stem cell reservoir for neurogenesis
    Article Snippet: The antibodies used in this study included mouse monoclonal anti-SETD4 (1:100, #sc-514060, Santa Cruz Biotechnology), rabbit polyclonal anti-GFAP (1:1000, #Z0334, Dako), goat polyclonal anti-GFAP (1:1000, #ab53554, Abcam), chicken polyclonal anti-NESTIN (1:1000, #NB100-1604, Novus Biologicals), mouse monoclonal anti-NESTIN (1:500, #MAB353, MilliporeSigma), rabbit polyclonal anti-SOX2 (1:200, #ab97959, Abcam), goat polyclonal anti-SOX2 (1:200, #AF2018, R&D Systems), rabbit monoclonal anti-KI67 (1:200, #ab16667, Abcam), mouse monoclonal antiPCNA (1:500, #ab29, Abcam), chicken polyclonal anti-GFP (1:1000, #ab13970, Abcam), rat monoclonal anti-BrdU (1:200, #ab6326, Abcam), goat polyclonal anti-DCX (1:500, #ab113435, Abcam), rabbit polyclonal anti-OLIG2 (1:500, #AB9610, MilliporeSigma), rabbit polyclonal anti-NEUN (1:1000, #ABN78, MilliporeSigma), mouse monoclonal antiMASH1 (1:200, #556604, BD Biosciences), chicken polyclonal anti-RFP (1:500, #600-901-379, Rockland immunochemicals), rabbit polyclonal anti-H4K20ME3 (1:500, #pAb-057-050, Diagenode), rabbit polyclonal antiH3K9AC (1:500, #ab10812, Abcam).

    Article Title: Interstitial-fluid shear stresses induced by vertically oscillating head motion lower blood pressure in hypertensive rats and humans.
    Article Snippet: Shuhei Murase, Naoyoshi Sakitani, Takahiro Maekawa, Daisuke Yoshino , Kouji Takano, Ayumu Konno , Hirokazu Hirai , Taku Saito , Sakae Tanaka, Keisuke Shinohara, Takuya Kishi, Yuki Yoshikawa , Takamasa Sakai , Makoto Ayaori, Hirohiko Inanami, Koji Tomiyasu, Atsushi Takashima, Toru Ogata , Hirotsugu Tsuchimochi , Shinya Sato, Shigeyoshi Saito, Kohzoh Yoshino, Yuiko Matsuura , Kenichi Funamoto , Hiroki Ochi, Masahiro Shinohara, Motoshi Nagao & Yasuhiro Sawada

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: Compound Sakura Finetek Cat# 4583 Vectashield Mounting Medium with DAPI Vector Laboratories Cat# H-1200-10 Critical commercial assays Jo urn al Pr e-p roo f Phosphorylated Neurofilament H Human ELISA BioVendor Cat# RD191138300R Corticosterone EIA IDS Cat# AC-14F1 BCA Protein Assay Kit Pierce Cat# 23225 Deposited data Raw data This paper https://doi.org/10.3494 5/F52P4M Experimental models: Organisms/strains Sprague-Dawley Rat Charles River Laboratories RRID: MGI: 5651135 Software and algorithms Kinema Tracer System Kissei Comtec http://www.kinematrac er.com/ Odyssey Application Software Version 3.0 LI-COR Biosciences RRID: SCR_014579 NIS Elements Imaging Software Nikon Instruments RRID: SCR_014329; https://calm.ucsf.edu/h ardware-and-softwareimage-analysis ImageJ NIH RRID: SCR_003070 AutoQuant Media Cybernetics RRID: SCR_002465 MetaMorph Automation and Image Analysis Software Molecular Devices RRID: SCR_002368 Adobe Photoshop Adobe RRID: SCR_014199 SPSS Statistics version 26.0 IBM RRID: SCR_016479 Graphpad Prism Graphpad RRID: SCR_002798 Adobe Illustrator Adobe RRID: SCR_010279 Other Infinite Horizon Impactor Precision Systems and Instrumentation IH-0400; https://psiimpactors.co m/product/ih400/ Treadmill Muromachi Kikai MK-680; https://muromachi.com /en/archives/english/19 36/ Biological Amplifier Nihon Kohden NEC Biotop 6R12; https://doi.org/10.1371/ journal.pone.0171937 AD Converter Cambridge Electronic Design Limited Micro 1401; https://ced.co.uk/produ cts/microwaveformio Constant Current Stimulator Digitimer DS7A; https://www.digitimer.c om/product/humanneurophysiology/periph eral-stimulators/ds7ads7ah-hv-currentstimulator/ Jo urn al P e-p roo f Plate Reader Tecan GENios; https://www.tecan.com/ customer-news/geniosan-innovativedetection-device-thatdelivers-flexibility-tothe-molecular-biologylaboratory-2347 Infrared Imaging System LI-COR Biosciences Odyssey Infrared Imaging System; RRID: SCR_023765; https://doi.org/10.1038/ s41598-024-70096-0 Cryostat Leica Biosystems CM1950; RRID: SCR_018061; https://www.leicabiosys tems.com/us/histologyequipment/cryostats/lei ca-cm1950/ Inverted Microscope Nikon Instruments ECLIPSE Ti inverted microscope system; RRID: SCR_021242; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Spinning Disk Confocal Microscope Yokogawa Electric Corporation CSU-22; RRID: SCR_020907; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Jo urn al Pr e-p roo f

    Article Title: Atp13a5 Marker Reveals Pericyte Specification in the Mouse Central Nervous System
    Article Snippet: Rabbit polyclonal anti-NeuN , Millipore Sigma , ABN78.

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: For assessment of lesion pathology in the experimental animal model, the tissue sections of the thoracic spinal cord segments were incubated with Jo urn al Pr e-p ro f 58 diluted primary antibodies: rabbit polyclonal anti-NeuN (1:500; Millipore; Cat# ABN78; RRID: AB_10807945), mouse monoclonal anti-GFAP (1:500; Millipore; Cat# MAB360; RRID: AB_11212597), rabbit monoclonal anti-Vimentin (1:50; Cell Signaling Technology; Cat# 5741; RRID: AB_10695459), and rabbit polyclonal anti-GAP43 (1:500; Millipore; Cat# AB5220; RRID: AB_2107282).

    Article Title: SETD4 cells contribute to brain development and maintain adult stem cell reservoir for neurogenesis
    Article Snippet: The antibodies used in this study included mouse monoclonal anti-SETD4 (1:100, #sc-514060, Santa Cruz Biotechnology), rabbit polyclonal anti-GFAP (1:1000, #Z0334, Dako), goat polyclonal anti-GFAP (1:1000, #ab53554, Abcam), chicken polyclonal anti-NESTIN (1:1000, #NB100-1604, Novus Biologicals), mouse monoclonal anti-NESTIN (1:500, #MAB353, MilliporeSigma), rabbit polyclonal anti-SOX2 (1:200, #ab97959, Abcam), goat polyclonal anti-SOX2 (1:200, #AF2018, R&D Systems), rabbit monoclonal anti-KI67 (1:200, #ab16667, Abcam), mouse monoclonal antiPCNA (1:500, #ab29, Abcam), chicken polyclonal anti-GFP (1:1000, #ab13970, Abcam), rat monoclonal anti-BrdU (1:200, #ab6326, Abcam), goat polyclonal anti-DCX (1:500, #ab113435, Abcam), rabbit polyclonal anti-OLIG2 (1:500, #AB9610, MilliporeSigma), rabbit polyclonal anti-NEUN (1:1000, #ABN78, MilliporeSigma), mouse monoclonal antiMASH1 (1:200, #556604, BD Biosciences), chicken polyclonal anti-RFP (1:500, #600-901-379, Rockland immunochemicals), rabbit polyclonal anti-H4K20ME3 (1:500, #pAb-057-050, Diagenode), rabbit polyclonal antiH3K9AC (1:500, #ab10812, Abcam).

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: Rabbit Polyclonal Anti-NeuN , Millipore , Cat# ABN78; RRID: AB_10807945.

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: For assessment of lesion pathology in the experimental animal model, the tissue sections of the thoracic spinal cord segments were incubated with diluted primary antibodies: rabbit polyclonal anti-NeuN (1:500; Millipore; Cat# ABN78; RRID: AB_10807945 ), mouse monoclonal anti-GFAP (1:500; Millipore; Cat# MAB360; RRID: AB_11212597 ), rabbit monoclonal anti-Vimentin (1:50; Cell Signaling Technology; Cat# 5741; RRID: AB_10695459 ), and rabbit polyclonal anti-GAP43 (1:500; Millipore; Cat# AB5220; RRID: AB_2107282 ).

    Microscopy:

    Article Title: SETD4 cells contribute to brain development and maintain adult stem cell reservoir for neurogenesis
    Article Snippet: The antibodies used in this study included mouse monoclonal anti-SETD4 (1:100, #sc-514060, Santa Cruz Biotechnology), rabbit polyclonal anti-GFAP (1:1000, #Z0334, Dako), goat polyclonal anti-GFAP (1:1000, #ab53554, Abcam), chicken polyclonal anti-NESTIN (1:1000, #NB100-1604, Novus Biologicals), mouse monoclonal anti-NESTIN (1:500, #MAB353, MilliporeSigma), rabbit polyclonal anti-SOX2 (1:200, #ab97959, Abcam), goat polyclonal anti-SOX2 (1:200, #AF2018, R&D Systems), rabbit monoclonal anti-KI67 (1:200, #ab16667, Abcam), mouse monoclonal antiPCNA (1:500, #ab29, Abcam), chicken polyclonal anti-GFP (1:1000, #ab13970, Abcam), rat monoclonal anti-BrdU (1:200, #ab6326, Abcam), goat polyclonal anti-DCX (1:500, #ab113435, Abcam), rabbit polyclonal anti-OLIG2 (1:500, #AB9610, MilliporeSigma), rabbit polyclonal anti-NEUN (1:1000, #ABN78, MilliporeSigma), mouse monoclonal antiMASH1 (1:200, #556604, BD Biosciences), chicken polyclonal anti-RFP (1:500, #600-901-379, Rockland immunochemicals), rabbit polyclonal anti-H4K20ME3 (1:500, #pAb-057-050, Diagenode), rabbit polyclonal antiH3K9AC (1:500, #ab10812, Abcam).

    Article Title: Interstitial-fluid shear stresses induced by vertically oscillating head motion lower blood pressure in hypertensive rats and humans.
    Article Snippet: Shuhei Murase, Naoyoshi Sakitani, Takahiro Maekawa, Daisuke Yoshino , Kouji Takano, Ayumu Konno , Hirokazu Hirai , Taku Saito , Sakae Tanaka, Keisuke Shinohara, Takuya Kishi, Yuki Yoshikawa , Takamasa Sakai , Makoto Ayaori, Hirohiko Inanami, Koji Tomiyasu, Atsushi Takashima, Toru Ogata , Hirotsugu Tsuchimochi , Shinya Sato, Shigeyoshi Saito, Kohzoh Yoshino, Yuiko Matsuura , Kenichi Funamoto , Hiroki Ochi, Masahiro Shinohara, Motoshi Nagao & Yasuhiro Sawada

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: Compound Sakura Finetek Cat# 4583 Vectashield Mounting Medium with DAPI Vector Laboratories Cat# H-1200-10 Critical commercial assays Jo urn al Pr e-p roo f Phosphorylated Neurofilament H Human ELISA BioVendor Cat# RD191138300R Corticosterone EIA IDS Cat# AC-14F1 BCA Protein Assay Kit Pierce Cat# 23225 Deposited data Raw data This paper https://doi.org/10.3494 5/F52P4M Experimental models: Organisms/strains Sprague-Dawley Rat Charles River Laboratories RRID: MGI: 5651135 Software and algorithms Kinema Tracer System Kissei Comtec http://www.kinematrac er.com/ Odyssey Application Software Version 3.0 LI-COR Biosciences RRID: SCR_014579 NIS Elements Imaging Software Nikon Instruments RRID: SCR_014329; https://calm.ucsf.edu/h ardware-and-softwareimage-analysis ImageJ NIH RRID: SCR_003070 AutoQuant Media Cybernetics RRID: SCR_002465 MetaMorph Automation and Image Analysis Software Molecular Devices RRID: SCR_002368 Adobe Photoshop Adobe RRID: SCR_014199 SPSS Statistics version 26.0 IBM RRID: SCR_016479 Graphpad Prism Graphpad RRID: SCR_002798 Adobe Illustrator Adobe RRID: SCR_010279 Other Infinite Horizon Impactor Precision Systems and Instrumentation IH-0400; https://psiimpactors.co m/product/ih400/ Treadmill Muromachi Kikai MK-680; https://muromachi.com /en/archives/english/19 36/ Biological Amplifier Nihon Kohden NEC Biotop 6R12; https://doi.org/10.1371/ journal.pone.0171937 AD Converter Cambridge Electronic Design Limited Micro 1401; https://ced.co.uk/produ cts/microwaveformio Constant Current Stimulator Digitimer DS7A; https://www.digitimer.c om/product/humanneurophysiology/periph eral-stimulators/ds7ads7ah-hv-currentstimulator/ Jo urn al P e-p roo f Plate Reader Tecan GENios; https://www.tecan.com/ customer-news/geniosan-innovativedetection-device-thatdelivers-flexibility-tothe-molecular-biologylaboratory-2347 Infrared Imaging System LI-COR Biosciences Odyssey Infrared Imaging System; RRID: SCR_023765; https://doi.org/10.1038/ s41598-024-70096-0 Cryostat Leica Biosystems CM1950; RRID: SCR_018061; https://www.leicabiosys tems.com/us/histologyequipment/cryostats/lei ca-cm1950/ Inverted Microscope Nikon Instruments ECLIPSE Ti inverted microscope system; RRID: SCR_021242; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Spinning Disk Confocal Microscope Yokogawa Electric Corporation CSU-22; RRID: SCR_020907; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Jo urn al Pr e-p roo f

    Article Title: Atp13a5 Marker Reveals Pericyte Specification in the Mouse Central Nervous System
    Article Snippet: Rabbit polyclonal anti-NeuN , Millipore Sigma , ABN78.

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: For assessment of lesion pathology in the experimental animal model, the tissue sections of the thoracic spinal cord segments were incubated with Jo urn al Pr e-p ro f 58 diluted primary antibodies: rabbit polyclonal anti-NeuN (1:500; Millipore; Cat# ABN78; RRID: AB_10807945), mouse monoclonal anti-GFAP (1:500; Millipore; Cat# MAB360; RRID: AB_11212597), rabbit monoclonal anti-Vimentin (1:50; Cell Signaling Technology; Cat# 5741; RRID: AB_10695459), and rabbit polyclonal anti-GAP43 (1:500; Millipore; Cat# AB5220; RRID: AB_2107282).

    Article Title: SETD4 cells contribute to brain development and maintain adult stem cell reservoir for neurogenesis
    Article Snippet: The antibodies used in this study included mouse monoclonal anti-SETD4 (1:100, #sc-514060, Santa Cruz Biotechnology), rabbit polyclonal anti-GFAP (1:1000, #Z0334, Dako), goat polyclonal anti-GFAP (1:1000, #ab53554, Abcam), chicken polyclonal anti-NESTIN (1:1000, #NB100-1604, Novus Biologicals), mouse monoclonal anti-NESTIN (1:500, #MAB353, MilliporeSigma), rabbit polyclonal anti-SOX2 (1:200, #ab97959, Abcam), goat polyclonal anti-SOX2 (1:200, #AF2018, R&D Systems), rabbit monoclonal anti-KI67 (1:200, #ab16667, Abcam), mouse monoclonal antiPCNA (1:500, #ab29, Abcam), chicken polyclonal anti-GFP (1:1000, #ab13970, Abcam), rat monoclonal anti-BrdU (1:200, #ab6326, Abcam), goat polyclonal anti-DCX (1:500, #ab113435, Abcam), rabbit polyclonal anti-OLIG2 (1:500, #AB9610, MilliporeSigma), rabbit polyclonal anti-NEUN (1:1000, #ABN78, MilliporeSigma), mouse monoclonal antiMASH1 (1:200, #556604, BD Biosciences), chicken polyclonal anti-RFP (1:500, #600-901-379, Rockland immunochemicals), rabbit polyclonal anti-H4K20ME3 (1:500, #pAb-057-050, Diagenode), rabbit polyclonal antiH3K9AC (1:500, #ab10812, Abcam).

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: Rabbit Polyclonal Anti-NeuN , Millipore , Cat# ABN78; RRID: AB_10807945.

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: For assessment of lesion pathology in the experimental animal model, the tissue sections of the thoracic spinal cord segments were incubated with diluted primary antibodies: rabbit polyclonal anti-NeuN (1:500; Millipore; Cat# ABN78; RRID: AB_10807945 ), mouse monoclonal anti-GFAP (1:500; Millipore; Cat# MAB360; RRID: AB_11212597 ), rabbit monoclonal anti-Vimentin (1:50; Cell Signaling Technology; Cat# 5741; RRID: AB_10695459 ), and rabbit polyclonal anti-GAP43 (1:500; Millipore; Cat# AB5220; RRID: AB_2107282 ).

    Animal Model:

    Article Title: SETD4 cells contribute to brain development and maintain adult stem cell reservoir for neurogenesis
    Article Snippet: The antibodies used in this study included mouse monoclonal anti-SETD4 (1:100, #sc-514060, Santa Cruz Biotechnology), rabbit polyclonal anti-GFAP (1:1000, #Z0334, Dako), goat polyclonal anti-GFAP (1:1000, #ab53554, Abcam), chicken polyclonal anti-NESTIN (1:1000, #NB100-1604, Novus Biologicals), mouse monoclonal anti-NESTIN (1:500, #MAB353, MilliporeSigma), rabbit polyclonal anti-SOX2 (1:200, #ab97959, Abcam), goat polyclonal anti-SOX2 (1:200, #AF2018, R&D Systems), rabbit monoclonal anti-KI67 (1:200, #ab16667, Abcam), mouse monoclonal antiPCNA (1:500, #ab29, Abcam), chicken polyclonal anti-GFP (1:1000, #ab13970, Abcam), rat monoclonal anti-BrdU (1:200, #ab6326, Abcam), goat polyclonal anti-DCX (1:500, #ab113435, Abcam), rabbit polyclonal anti-OLIG2 (1:500, #AB9610, MilliporeSigma), rabbit polyclonal anti-NEUN (1:1000, #ABN78, MilliporeSigma), mouse monoclonal antiMASH1 (1:200, #556604, BD Biosciences), chicken polyclonal anti-RFP (1:500, #600-901-379, Rockland immunochemicals), rabbit polyclonal anti-H4K20ME3 (1:500, #pAb-057-050, Diagenode), rabbit polyclonal antiH3K9AC (1:500, #ab10812, Abcam).

    Article Title: Interstitial-fluid shear stresses induced by vertically oscillating head motion lower blood pressure in hypertensive rats and humans.
    Article Snippet: Shuhei Murase, Naoyoshi Sakitani, Takahiro Maekawa, Daisuke Yoshino , Kouji Takano, Ayumu Konno , Hirokazu Hirai , Taku Saito , Sakae Tanaka, Keisuke Shinohara, Takuya Kishi, Yuki Yoshikawa , Takamasa Sakai , Makoto Ayaori, Hirohiko Inanami, Koji Tomiyasu, Atsushi Takashima, Toru Ogata , Hirotsugu Tsuchimochi , Shinya Sato, Shigeyoshi Saito, Kohzoh Yoshino, Yuiko Matsuura , Kenichi Funamoto , Hiroki Ochi, Masahiro Shinohara, Motoshi Nagao & Yasuhiro Sawada

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: Compound Sakura Finetek Cat# 4583 Vectashield Mounting Medium with DAPI Vector Laboratories Cat# H-1200-10 Critical commercial assays Jo urn al Pr e-p roo f Phosphorylated Neurofilament H Human ELISA BioVendor Cat# RD191138300R Corticosterone EIA IDS Cat# AC-14F1 BCA Protein Assay Kit Pierce Cat# 23225 Deposited data Raw data This paper https://doi.org/10.3494 5/F52P4M Experimental models: Organisms/strains Sprague-Dawley Rat Charles River Laboratories RRID: MGI: 5651135 Software and algorithms Kinema Tracer System Kissei Comtec http://www.kinematrac er.com/ Odyssey Application Software Version 3.0 LI-COR Biosciences RRID: SCR_014579 NIS Elements Imaging Software Nikon Instruments RRID: SCR_014329; https://calm.ucsf.edu/h ardware-and-softwareimage-analysis ImageJ NIH RRID: SCR_003070 AutoQuant Media Cybernetics RRID: SCR_002465 MetaMorph Automation and Image Analysis Software Molecular Devices RRID: SCR_002368 Adobe Photoshop Adobe RRID: SCR_014199 SPSS Statistics version 26.0 IBM RRID: SCR_016479 Graphpad Prism Graphpad RRID: SCR_002798 Adobe Illustrator Adobe RRID: SCR_010279 Other Infinite Horizon Impactor Precision Systems and Instrumentation IH-0400; https://psiimpactors.co m/product/ih400/ Treadmill Muromachi Kikai MK-680; https://muromachi.com /en/archives/english/19 36/ Biological Amplifier Nihon Kohden NEC Biotop 6R12; https://doi.org/10.1371/ journal.pone.0171937 AD Converter Cambridge Electronic Design Limited Micro 1401; https://ced.co.uk/produ cts/microwaveformio Constant Current Stimulator Digitimer DS7A; https://www.digitimer.c om/product/humanneurophysiology/periph eral-stimulators/ds7ads7ah-hv-currentstimulator/ Jo urn al P e-p roo f Plate Reader Tecan GENios; https://www.tecan.com/ customer-news/geniosan-innovativedetection-device-thatdelivers-flexibility-tothe-molecular-biologylaboratory-2347 Infrared Imaging System LI-COR Biosciences Odyssey Infrared Imaging System; RRID: SCR_023765; https://doi.org/10.1038/ s41598-024-70096-0 Cryostat Leica Biosystems CM1950; RRID: SCR_018061; https://www.leicabiosys tems.com/us/histologyequipment/cryostats/lei ca-cm1950/ Inverted Microscope Nikon Instruments ECLIPSE Ti inverted microscope system; RRID: SCR_021242; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Spinning Disk Confocal Microscope Yokogawa Electric Corporation CSU-22; RRID: SCR_020907; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Jo urn al Pr e-p roo f

    Article Title: Atp13a5 Marker Reveals Pericyte Specification in the Mouse Central Nervous System
    Article Snippet: Rabbit polyclonal anti-NeuN , Millipore Sigma , ABN78.

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: For assessment of lesion pathology in the experimental animal model, the tissue sections of the thoracic spinal cord segments were incubated with Jo urn al Pr e-p ro f 58 diluted primary antibodies: rabbit polyclonal anti-NeuN (1:500; Millipore; Cat# ABN78; RRID: AB_10807945), mouse monoclonal anti-GFAP (1:500; Millipore; Cat# MAB360; RRID: AB_11212597), rabbit monoclonal anti-Vimentin (1:50; Cell Signaling Technology; Cat# 5741; RRID: AB_10695459), and rabbit polyclonal anti-GAP43 (1:500; Millipore; Cat# AB5220; RRID: AB_2107282).

    Article Title: SETD4 cells contribute to brain development and maintain adult stem cell reservoir for neurogenesis
    Article Snippet: The antibodies used in this study included mouse monoclonal anti-SETD4 (1:100, #sc-514060, Santa Cruz Biotechnology), rabbit polyclonal anti-GFAP (1:1000, #Z0334, Dako), goat polyclonal anti-GFAP (1:1000, #ab53554, Abcam), chicken polyclonal anti-NESTIN (1:1000, #NB100-1604, Novus Biologicals), mouse monoclonal anti-NESTIN (1:500, #MAB353, MilliporeSigma), rabbit polyclonal anti-SOX2 (1:200, #ab97959, Abcam), goat polyclonal anti-SOX2 (1:200, #AF2018, R&D Systems), rabbit monoclonal anti-KI67 (1:200, #ab16667, Abcam), mouse monoclonal antiPCNA (1:500, #ab29, Abcam), chicken polyclonal anti-GFP (1:1000, #ab13970, Abcam), rat monoclonal anti-BrdU (1:200, #ab6326, Abcam), goat polyclonal anti-DCX (1:500, #ab113435, Abcam), rabbit polyclonal anti-OLIG2 (1:500, #AB9610, MilliporeSigma), rabbit polyclonal anti-NEUN (1:1000, #ABN78, MilliporeSigma), mouse monoclonal antiMASH1 (1:200, #556604, BD Biosciences), chicken polyclonal anti-RFP (1:500, #600-901-379, Rockland immunochemicals), rabbit polyclonal anti-H4K20ME3 (1:500, #pAb-057-050, Diagenode), rabbit polyclonal antiH3K9AC (1:500, #ab10812, Abcam).

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: Rabbit Polyclonal Anti-NeuN , Millipore , Cat# ABN78; RRID: AB_10807945.

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: For assessment of lesion pathology in the experimental animal model, the tissue sections of the thoracic spinal cord segments were incubated with diluted primary antibodies: rabbit polyclonal anti-NeuN (1:500; Millipore; Cat# ABN78; RRID: AB_10807945 ), mouse monoclonal anti-GFAP (1:500; Millipore; Cat# MAB360; RRID: AB_11212597 ), rabbit monoclonal anti-Vimentin (1:50; Cell Signaling Technology; Cat# 5741; RRID: AB_10695459 ), and rabbit polyclonal anti-GAP43 (1:500; Millipore; Cat# AB5220; RRID: AB_2107282 ).

    Incubation:

    Article Title: SETD4 cells contribute to brain development and maintain adult stem cell reservoir for neurogenesis
    Article Snippet: The antibodies used in this study included mouse monoclonal anti-SETD4 (1:100, #sc-514060, Santa Cruz Biotechnology), rabbit polyclonal anti-GFAP (1:1000, #Z0334, Dako), goat polyclonal anti-GFAP (1:1000, #ab53554, Abcam), chicken polyclonal anti-NESTIN (1:1000, #NB100-1604, Novus Biologicals), mouse monoclonal anti-NESTIN (1:500, #MAB353, MilliporeSigma), rabbit polyclonal anti-SOX2 (1:200, #ab97959, Abcam), goat polyclonal anti-SOX2 (1:200, #AF2018, R&D Systems), rabbit monoclonal anti-KI67 (1:200, #ab16667, Abcam), mouse monoclonal antiPCNA (1:500, #ab29, Abcam), chicken polyclonal anti-GFP (1:1000, #ab13970, Abcam), rat monoclonal anti-BrdU (1:200, #ab6326, Abcam), goat polyclonal anti-DCX (1:500, #ab113435, Abcam), rabbit polyclonal anti-OLIG2 (1:500, #AB9610, MilliporeSigma), rabbit polyclonal anti-NEUN (1:1000, #ABN78, MilliporeSigma), mouse monoclonal antiMASH1 (1:200, #556604, BD Biosciences), chicken polyclonal anti-RFP (1:500, #600-901-379, Rockland immunochemicals), rabbit polyclonal anti-H4K20ME3 (1:500, #pAb-057-050, Diagenode), rabbit polyclonal antiH3K9AC (1:500, #ab10812, Abcam).

    Article Title: Interstitial-fluid shear stresses induced by vertically oscillating head motion lower blood pressure in hypertensive rats and humans.
    Article Snippet: Shuhei Murase, Naoyoshi Sakitani, Takahiro Maekawa, Daisuke Yoshino , Kouji Takano, Ayumu Konno , Hirokazu Hirai , Taku Saito , Sakae Tanaka, Keisuke Shinohara, Takuya Kishi, Yuki Yoshikawa , Takamasa Sakai , Makoto Ayaori, Hirohiko Inanami, Koji Tomiyasu, Atsushi Takashima, Toru Ogata , Hirotsugu Tsuchimochi , Shinya Sato, Shigeyoshi Saito, Kohzoh Yoshino, Yuiko Matsuura , Kenichi Funamoto , Hiroki Ochi, Masahiro Shinohara, Motoshi Nagao & Yasuhiro Sawada

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: Compound Sakura Finetek Cat# 4583 Vectashield Mounting Medium with DAPI Vector Laboratories Cat# H-1200-10 Critical commercial assays Jo urn al Pr e-p roo f Phosphorylated Neurofilament H Human ELISA BioVendor Cat# RD191138300R Corticosterone EIA IDS Cat# AC-14F1 BCA Protein Assay Kit Pierce Cat# 23225 Deposited data Raw data This paper https://doi.org/10.3494 5/F52P4M Experimental models: Organisms/strains Sprague-Dawley Rat Charles River Laboratories RRID: MGI: 5651135 Software and algorithms Kinema Tracer System Kissei Comtec http://www.kinematrac er.com/ Odyssey Application Software Version 3.0 LI-COR Biosciences RRID: SCR_014579 NIS Elements Imaging Software Nikon Instruments RRID: SCR_014329; https://calm.ucsf.edu/h ardware-and-softwareimage-analysis ImageJ NIH RRID: SCR_003070 AutoQuant Media Cybernetics RRID: SCR_002465 MetaMorph Automation and Image Analysis Software Molecular Devices RRID: SCR_002368 Adobe Photoshop Adobe RRID: SCR_014199 SPSS Statistics version 26.0 IBM RRID: SCR_016479 Graphpad Prism Graphpad RRID: SCR_002798 Adobe Illustrator Adobe RRID: SCR_010279 Other Infinite Horizon Impactor Precision Systems and Instrumentation IH-0400; https://psiimpactors.co m/product/ih400/ Treadmill Muromachi Kikai MK-680; https://muromachi.com /en/archives/english/19 36/ Biological Amplifier Nihon Kohden NEC Biotop 6R12; https://doi.org/10.1371/ journal.pone.0171937 AD Converter Cambridge Electronic Design Limited Micro 1401; https://ced.co.uk/produ cts/microwaveformio Constant Current Stimulator Digitimer DS7A; https://www.digitimer.c om/product/humanneurophysiology/periph eral-stimulators/ds7ads7ah-hv-currentstimulator/ Jo urn al P e-p roo f Plate Reader Tecan GENios; https://www.tecan.com/ customer-news/geniosan-innovativedetection-device-thatdelivers-flexibility-tothe-molecular-biologylaboratory-2347 Infrared Imaging System LI-COR Biosciences Odyssey Infrared Imaging System; RRID: SCR_023765; https://doi.org/10.1038/ s41598-024-70096-0 Cryostat Leica Biosystems CM1950; RRID: SCR_018061; https://www.leicabiosys tems.com/us/histologyequipment/cryostats/lei ca-cm1950/ Inverted Microscope Nikon Instruments ECLIPSE Ti inverted microscope system; RRID: SCR_021242; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Spinning Disk Confocal Microscope Yokogawa Electric Corporation CSU-22; RRID: SCR_020907; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Jo urn al Pr e-p roo f

    Article Title: Atp13a5 Marker Reveals Pericyte Specification in the Mouse Central Nervous System
    Article Snippet: Rabbit polyclonal anti-NeuN , Millipore Sigma , ABN78.

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: For assessment of lesion pathology in the experimental animal model, the tissue sections of the thoracic spinal cord segments were incubated with Jo urn al Pr e-p ro f 58 diluted primary antibodies: rabbit polyclonal anti-NeuN (1:500; Millipore; Cat# ABN78; RRID: AB_10807945), mouse monoclonal anti-GFAP (1:500; Millipore; Cat# MAB360; RRID: AB_11212597), rabbit monoclonal anti-Vimentin (1:50; Cell Signaling Technology; Cat# 5741; RRID: AB_10695459), and rabbit polyclonal anti-GAP43 (1:500; Millipore; Cat# AB5220; RRID: AB_2107282).

    Article Title: SETD4 cells contribute to brain development and maintain adult stem cell reservoir for neurogenesis
    Article Snippet: The antibodies used in this study included mouse monoclonal anti-SETD4 (1:100, #sc-514060, Santa Cruz Biotechnology), rabbit polyclonal anti-GFAP (1:1000, #Z0334, Dako), goat polyclonal anti-GFAP (1:1000, #ab53554, Abcam), chicken polyclonal anti-NESTIN (1:1000, #NB100-1604, Novus Biologicals), mouse monoclonal anti-NESTIN (1:500, #MAB353, MilliporeSigma), rabbit polyclonal anti-SOX2 (1:200, #ab97959, Abcam), goat polyclonal anti-SOX2 (1:200, #AF2018, R&D Systems), rabbit monoclonal anti-KI67 (1:200, #ab16667, Abcam), mouse monoclonal antiPCNA (1:500, #ab29, Abcam), chicken polyclonal anti-GFP (1:1000, #ab13970, Abcam), rat monoclonal anti-BrdU (1:200, #ab6326, Abcam), goat polyclonal anti-DCX (1:500, #ab113435, Abcam), rabbit polyclonal anti-OLIG2 (1:500, #AB9610, MilliporeSigma), rabbit polyclonal anti-NEUN (1:1000, #ABN78, MilliporeSigma), mouse monoclonal antiMASH1 (1:200, #556604, BD Biosciences), chicken polyclonal anti-RFP (1:500, #600-901-379, Rockland immunochemicals), rabbit polyclonal anti-H4K20ME3 (1:500, #pAb-057-050, Diagenode), rabbit polyclonal antiH3K9AC (1:500, #ab10812, Abcam).

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: Rabbit Polyclonal Anti-NeuN , Millipore , Cat# ABN78; RRID: AB_10807945.

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: For assessment of lesion pathology in the experimental animal model, the tissue sections of the thoracic spinal cord segments were incubated with diluted primary antibodies: rabbit polyclonal anti-NeuN (1:500; Millipore; Cat# ABN78; RRID: AB_10807945 ), mouse monoclonal anti-GFAP (1:500; Millipore; Cat# MAB360; RRID: AB_11212597 ), rabbit monoclonal anti-Vimentin (1:50; Cell Signaling Technology; Cat# 5741; RRID: AB_10695459 ), and rabbit polyclonal anti-GAP43 (1:500; Millipore; Cat# AB5220; RRID: AB_2107282 ).

    Gene Expression:

    Article Title: SETD4 cells contribute to brain development and maintain adult stem cell reservoir for neurogenesis
    Article Snippet: The antibodies used in this study included mouse monoclonal anti-SETD4 (1:100, #sc-514060, Santa Cruz Biotechnology), rabbit polyclonal anti-GFAP (1:1000, #Z0334, Dako), goat polyclonal anti-GFAP (1:1000, #ab53554, Abcam), chicken polyclonal anti-NESTIN (1:1000, #NB100-1604, Novus Biologicals), mouse monoclonal anti-NESTIN (1:500, #MAB353, MilliporeSigma), rabbit polyclonal anti-SOX2 (1:200, #ab97959, Abcam), goat polyclonal anti-SOX2 (1:200, #AF2018, R&D Systems), rabbit monoclonal anti-KI67 (1:200, #ab16667, Abcam), mouse monoclonal antiPCNA (1:500, #ab29, Abcam), chicken polyclonal anti-GFP (1:1000, #ab13970, Abcam), rat monoclonal anti-BrdU (1:200, #ab6326, Abcam), goat polyclonal anti-DCX (1:500, #ab113435, Abcam), rabbit polyclonal anti-OLIG2 (1:500, #AB9610, MilliporeSigma), rabbit polyclonal anti-NEUN (1:1000, #ABN78, MilliporeSigma), mouse monoclonal antiMASH1 (1:200, #556604, BD Biosciences), chicken polyclonal anti-RFP (1:500, #600-901-379, Rockland immunochemicals), rabbit polyclonal anti-H4K20ME3 (1:500, #pAb-057-050, Diagenode), rabbit polyclonal antiH3K9AC (1:500, #ab10812, Abcam).

    Article Title: Interstitial-fluid shear stresses induced by vertically oscillating head motion lower blood pressure in hypertensive rats and humans.
    Article Snippet: Shuhei Murase, Naoyoshi Sakitani, Takahiro Maekawa, Daisuke Yoshino , Kouji Takano, Ayumu Konno , Hirokazu Hirai , Taku Saito , Sakae Tanaka, Keisuke Shinohara, Takuya Kishi, Yuki Yoshikawa , Takamasa Sakai , Makoto Ayaori, Hirohiko Inanami, Koji Tomiyasu, Atsushi Takashima, Toru Ogata , Hirotsugu Tsuchimochi , Shinya Sato, Shigeyoshi Saito, Kohzoh Yoshino, Yuiko Matsuura , Kenichi Funamoto , Hiroki Ochi, Masahiro Shinohara, Motoshi Nagao & Yasuhiro Sawada

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: Compound Sakura Finetek Cat# 4583 Vectashield Mounting Medium with DAPI Vector Laboratories Cat# H-1200-10 Critical commercial assays Jo urn al Pr e-p roo f Phosphorylated Neurofilament H Human ELISA BioVendor Cat# RD191138300R Corticosterone EIA IDS Cat# AC-14F1 BCA Protein Assay Kit Pierce Cat# 23225 Deposited data Raw data This paper https://doi.org/10.3494 5/F52P4M Experimental models: Organisms/strains Sprague-Dawley Rat Charles River Laboratories RRID: MGI: 5651135 Software and algorithms Kinema Tracer System Kissei Comtec http://www.kinematrac er.com/ Odyssey Application Software Version 3.0 LI-COR Biosciences RRID: SCR_014579 NIS Elements Imaging Software Nikon Instruments RRID: SCR_014329; https://calm.ucsf.edu/h ardware-and-softwareimage-analysis ImageJ NIH RRID: SCR_003070 AutoQuant Media Cybernetics RRID: SCR_002465 MetaMorph Automation and Image Analysis Software Molecular Devices RRID: SCR_002368 Adobe Photoshop Adobe RRID: SCR_014199 SPSS Statistics version 26.0 IBM RRID: SCR_016479 Graphpad Prism Graphpad RRID: SCR_002798 Adobe Illustrator Adobe RRID: SCR_010279 Other Infinite Horizon Impactor Precision Systems and Instrumentation IH-0400; https://psiimpactors.co m/product/ih400/ Treadmill Muromachi Kikai MK-680; https://muromachi.com /en/archives/english/19 36/ Biological Amplifier Nihon Kohden NEC Biotop 6R12; https://doi.org/10.1371/ journal.pone.0171937 AD Converter Cambridge Electronic Design Limited Micro 1401; https://ced.co.uk/produ cts/microwaveformio Constant Current Stimulator Digitimer DS7A; https://www.digitimer.c om/product/humanneurophysiology/periph eral-stimulators/ds7ads7ah-hv-currentstimulator/ Jo urn al P e-p roo f Plate Reader Tecan GENios; https://www.tecan.com/ customer-news/geniosan-innovativedetection-device-thatdelivers-flexibility-tothe-molecular-biologylaboratory-2347 Infrared Imaging System LI-COR Biosciences Odyssey Infrared Imaging System; RRID: SCR_023765; https://doi.org/10.1038/ s41598-024-70096-0 Cryostat Leica Biosystems CM1950; RRID: SCR_018061; https://www.leicabiosys tems.com/us/histologyequipment/cryostats/lei ca-cm1950/ Inverted Microscope Nikon Instruments ECLIPSE Ti inverted microscope system; RRID: SCR_021242; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Spinning Disk Confocal Microscope Yokogawa Electric Corporation CSU-22; RRID: SCR_020907; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Jo urn al Pr e-p roo f

    Article Title: Atp13a5 Marker Reveals Pericyte Specification in the Mouse Central Nervous System
    Article Snippet: Rabbit polyclonal anti-NeuN , Millipore Sigma , ABN78.

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: For assessment of lesion pathology in the experimental animal model, the tissue sections of the thoracic spinal cord segments were incubated with Jo urn al Pr e-p ro f 58 diluted primary antibodies: rabbit polyclonal anti-NeuN (1:500; Millipore; Cat# ABN78; RRID: AB_10807945), mouse monoclonal anti-GFAP (1:500; Millipore; Cat# MAB360; RRID: AB_11212597), rabbit monoclonal anti-Vimentin (1:50; Cell Signaling Technology; Cat# 5741; RRID: AB_10695459), and rabbit polyclonal anti-GAP43 (1:500; Millipore; Cat# AB5220; RRID: AB_2107282).

    Article Title: SETD4 cells contribute to brain development and maintain adult stem cell reservoir for neurogenesis
    Article Snippet: The antibodies used in this study included mouse monoclonal anti-SETD4 (1:100, #sc-514060, Santa Cruz Biotechnology), rabbit polyclonal anti-GFAP (1:1000, #Z0334, Dako), goat polyclonal anti-GFAP (1:1000, #ab53554, Abcam), chicken polyclonal anti-NESTIN (1:1000, #NB100-1604, Novus Biologicals), mouse monoclonal anti-NESTIN (1:500, #MAB353, MilliporeSigma), rabbit polyclonal anti-SOX2 (1:200, #ab97959, Abcam), goat polyclonal anti-SOX2 (1:200, #AF2018, R&D Systems), rabbit monoclonal anti-KI67 (1:200, #ab16667, Abcam), mouse monoclonal antiPCNA (1:500, #ab29, Abcam), chicken polyclonal anti-GFP (1:1000, #ab13970, Abcam), rat monoclonal anti-BrdU (1:200, #ab6326, Abcam), goat polyclonal anti-DCX (1:500, #ab113435, Abcam), rabbit polyclonal anti-OLIG2 (1:500, #AB9610, MilliporeSigma), rabbit polyclonal anti-NEUN (1:1000, #ABN78, MilliporeSigma), mouse monoclonal antiMASH1 (1:200, #556604, BD Biosciences), chicken polyclonal anti-RFP (1:500, #600-901-379, Rockland immunochemicals), rabbit polyclonal anti-H4K20ME3 (1:500, #pAb-057-050, Diagenode), rabbit polyclonal antiH3K9AC (1:500, #ab10812, Abcam).

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: Rabbit Polyclonal Anti-NeuN , Millipore , Cat# ABN78; RRID: AB_10807945.

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: For assessment of lesion pathology in the experimental animal model, the tissue sections of the thoracic spinal cord segments were incubated with diluted primary antibodies: rabbit polyclonal anti-NeuN (1:500; Millipore; Cat# ABN78; RRID: AB_10807945 ), mouse monoclonal anti-GFAP (1:500; Millipore; Cat# MAB360; RRID: AB_11212597 ), rabbit monoclonal anti-Vimentin (1:50; Cell Signaling Technology; Cat# 5741; RRID: AB_10695459 ), and rabbit polyclonal anti-GAP43 (1:500; Millipore; Cat# AB5220; RRID: AB_2107282 ).

    DNA Extraction:

    Article Title: SETD4 cells contribute to brain development and maintain adult stem cell reservoir for neurogenesis
    Article Snippet: The antibodies used in this study included mouse monoclonal anti-SETD4 (1:100, #sc-514060, Santa Cruz Biotechnology), rabbit polyclonal anti-GFAP (1:1000, #Z0334, Dako), goat polyclonal anti-GFAP (1:1000, #ab53554, Abcam), chicken polyclonal anti-NESTIN (1:1000, #NB100-1604, Novus Biologicals), mouse monoclonal anti-NESTIN (1:500, #MAB353, MilliporeSigma), rabbit polyclonal anti-SOX2 (1:200, #ab97959, Abcam), goat polyclonal anti-SOX2 (1:200, #AF2018, R&D Systems), rabbit monoclonal anti-KI67 (1:200, #ab16667, Abcam), mouse monoclonal antiPCNA (1:500, #ab29, Abcam), chicken polyclonal anti-GFP (1:1000, #ab13970, Abcam), rat monoclonal anti-BrdU (1:200, #ab6326, Abcam), goat polyclonal anti-DCX (1:500, #ab113435, Abcam), rabbit polyclonal anti-OLIG2 (1:500, #AB9610, MilliporeSigma), rabbit polyclonal anti-NEUN (1:1000, #ABN78, MilliporeSigma), mouse monoclonal antiMASH1 (1:200, #556604, BD Biosciences), chicken polyclonal anti-RFP (1:500, #600-901-379, Rockland immunochemicals), rabbit polyclonal anti-H4K20ME3 (1:500, #pAb-057-050, Diagenode), rabbit polyclonal antiH3K9AC (1:500, #ab10812, Abcam).

    Article Title: Interstitial-fluid shear stresses induced by vertically oscillating head motion lower blood pressure in hypertensive rats and humans.
    Article Snippet: Shuhei Murase, Naoyoshi Sakitani, Takahiro Maekawa, Daisuke Yoshino , Kouji Takano, Ayumu Konno , Hirokazu Hirai , Taku Saito , Sakae Tanaka, Keisuke Shinohara, Takuya Kishi, Yuki Yoshikawa , Takamasa Sakai , Makoto Ayaori, Hirohiko Inanami, Koji Tomiyasu, Atsushi Takashima, Toru Ogata , Hirotsugu Tsuchimochi , Shinya Sato, Shigeyoshi Saito, Kohzoh Yoshino, Yuiko Matsuura , Kenichi Funamoto , Hiroki Ochi, Masahiro Shinohara, Motoshi Nagao & Yasuhiro Sawada

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: Compound Sakura Finetek Cat# 4583 Vectashield Mounting Medium with DAPI Vector Laboratories Cat# H-1200-10 Critical commercial assays Jo urn al Pr e-p roo f Phosphorylated Neurofilament H Human ELISA BioVendor Cat# RD191138300R Corticosterone EIA IDS Cat# AC-14F1 BCA Protein Assay Kit Pierce Cat# 23225 Deposited data Raw data This paper https://doi.org/10.3494 5/F52P4M Experimental models: Organisms/strains Sprague-Dawley Rat Charles River Laboratories RRID: MGI: 5651135 Software and algorithms Kinema Tracer System Kissei Comtec http://www.kinematrac er.com/ Odyssey Application Software Version 3.0 LI-COR Biosciences RRID: SCR_014579 NIS Elements Imaging Software Nikon Instruments RRID: SCR_014329; https://calm.ucsf.edu/h ardware-and-softwareimage-analysis ImageJ NIH RRID: SCR_003070 AutoQuant Media Cybernetics RRID: SCR_002465 MetaMorph Automation and Image Analysis Software Molecular Devices RRID: SCR_002368 Adobe Photoshop Adobe RRID: SCR_014199 SPSS Statistics version 26.0 IBM RRID: SCR_016479 Graphpad Prism Graphpad RRID: SCR_002798 Adobe Illustrator Adobe RRID: SCR_010279 Other Infinite Horizon Impactor Precision Systems and Instrumentation IH-0400; https://psiimpactors.co m/product/ih400/ Treadmill Muromachi Kikai MK-680; https://muromachi.com /en/archives/english/19 36/ Biological Amplifier Nihon Kohden NEC Biotop 6R12; https://doi.org/10.1371/ journal.pone.0171937 AD Converter Cambridge Electronic Design Limited Micro 1401; https://ced.co.uk/produ cts/microwaveformio Constant Current Stimulator Digitimer DS7A; https://www.digitimer.c om/product/humanneurophysiology/periph eral-stimulators/ds7ads7ah-hv-currentstimulator/ Jo urn al P e-p roo f Plate Reader Tecan GENios; https://www.tecan.com/ customer-news/geniosan-innovativedetection-device-thatdelivers-flexibility-tothe-molecular-biologylaboratory-2347 Infrared Imaging System LI-COR Biosciences Odyssey Infrared Imaging System; RRID: SCR_023765; https://doi.org/10.1038/ s41598-024-70096-0 Cryostat Leica Biosystems CM1950; RRID: SCR_018061; https://www.leicabiosys tems.com/us/histologyequipment/cryostats/lei ca-cm1950/ Inverted Microscope Nikon Instruments ECLIPSE Ti inverted microscope system; RRID: SCR_021242; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Spinning Disk Confocal Microscope Yokogawa Electric Corporation CSU-22; RRID: SCR_020907; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Jo urn al Pr e-p roo f

    Article Title: Atp13a5 Marker Reveals Pericyte Specification in the Mouse Central Nervous System
    Article Snippet: Rabbit polyclonal anti-NeuN , Millipore Sigma , ABN78.

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: For assessment of lesion pathology in the experimental animal model, the tissue sections of the thoracic spinal cord segments were incubated with Jo urn al Pr e-p ro f 58 diluted primary antibodies: rabbit polyclonal anti-NeuN (1:500; Millipore; Cat# ABN78; RRID: AB_10807945), mouse monoclonal anti-GFAP (1:500; Millipore; Cat# MAB360; RRID: AB_11212597), rabbit monoclonal anti-Vimentin (1:50; Cell Signaling Technology; Cat# 5741; RRID: AB_10695459), and rabbit polyclonal anti-GAP43 (1:500; Millipore; Cat# AB5220; RRID: AB_2107282).

    Article Title: SETD4 cells contribute to brain development and maintain adult stem cell reservoir for neurogenesis
    Article Snippet: The antibodies used in this study included mouse monoclonal anti-SETD4 (1:100, #sc-514060, Santa Cruz Biotechnology), rabbit polyclonal anti-GFAP (1:1000, #Z0334, Dako), goat polyclonal anti-GFAP (1:1000, #ab53554, Abcam), chicken polyclonal anti-NESTIN (1:1000, #NB100-1604, Novus Biologicals), mouse monoclonal anti-NESTIN (1:500, #MAB353, MilliporeSigma), rabbit polyclonal anti-SOX2 (1:200, #ab97959, Abcam), goat polyclonal anti-SOX2 (1:200, #AF2018, R&D Systems), rabbit monoclonal anti-KI67 (1:200, #ab16667, Abcam), mouse monoclonal antiPCNA (1:500, #ab29, Abcam), chicken polyclonal anti-GFP (1:1000, #ab13970, Abcam), rat monoclonal anti-BrdU (1:200, #ab6326, Abcam), goat polyclonal anti-DCX (1:500, #ab113435, Abcam), rabbit polyclonal anti-OLIG2 (1:500, #AB9610, MilliporeSigma), rabbit polyclonal anti-NEUN (1:1000, #ABN78, MilliporeSigma), mouse monoclonal antiMASH1 (1:200, #556604, BD Biosciences), chicken polyclonal anti-RFP (1:500, #600-901-379, Rockland immunochemicals), rabbit polyclonal anti-H4K20ME3 (1:500, #pAb-057-050, Diagenode), rabbit polyclonal antiH3K9AC (1:500, #ab10812, Abcam).

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: Rabbit Polyclonal Anti-NeuN , Millipore , Cat# ABN78; RRID: AB_10807945.

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: For assessment of lesion pathology in the experimental animal model, the tissue sections of the thoracic spinal cord segments were incubated with diluted primary antibodies: rabbit polyclonal anti-NeuN (1:500; Millipore; Cat# ABN78; RRID: AB_10807945 ), mouse monoclonal anti-GFAP (1:500; Millipore; Cat# MAB360; RRID: AB_11212597 ), rabbit monoclonal anti-Vimentin (1:50; Cell Signaling Technology; Cat# 5741; RRID: AB_10695459 ), and rabbit polyclonal anti-GAP43 (1:500; Millipore; Cat# AB5220; RRID: AB_2107282 ).

    Isolation:

    Article Title: SETD4 cells contribute to brain development and maintain adult stem cell reservoir for neurogenesis
    Article Snippet: The antibodies used in this study included mouse monoclonal anti-SETD4 (1:100, #sc-514060, Santa Cruz Biotechnology), rabbit polyclonal anti-GFAP (1:1000, #Z0334, Dako), goat polyclonal anti-GFAP (1:1000, #ab53554, Abcam), chicken polyclonal anti-NESTIN (1:1000, #NB100-1604, Novus Biologicals), mouse monoclonal anti-NESTIN (1:500, #MAB353, MilliporeSigma), rabbit polyclonal anti-SOX2 (1:200, #ab97959, Abcam), goat polyclonal anti-SOX2 (1:200, #AF2018, R&D Systems), rabbit monoclonal anti-KI67 (1:200, #ab16667, Abcam), mouse monoclonal antiPCNA (1:500, #ab29, Abcam), chicken polyclonal anti-GFP (1:1000, #ab13970, Abcam), rat monoclonal anti-BrdU (1:200, #ab6326, Abcam), goat polyclonal anti-DCX (1:500, #ab113435, Abcam), rabbit polyclonal anti-OLIG2 (1:500, #AB9610, MilliporeSigma), rabbit polyclonal anti-NEUN (1:1000, #ABN78, MilliporeSigma), mouse monoclonal antiMASH1 (1:200, #556604, BD Biosciences), chicken polyclonal anti-RFP (1:500, #600-901-379, Rockland immunochemicals), rabbit polyclonal anti-H4K20ME3 (1:500, #pAb-057-050, Diagenode), rabbit polyclonal antiH3K9AC (1:500, #ab10812, Abcam).

    Article Title: Interstitial-fluid shear stresses induced by vertically oscillating head motion lower blood pressure in hypertensive rats and humans.
    Article Snippet: Shuhei Murase, Naoyoshi Sakitani, Takahiro Maekawa, Daisuke Yoshino , Kouji Takano, Ayumu Konno , Hirokazu Hirai , Taku Saito , Sakae Tanaka, Keisuke Shinohara, Takuya Kishi, Yuki Yoshikawa , Takamasa Sakai , Makoto Ayaori, Hirohiko Inanami, Koji Tomiyasu, Atsushi Takashima, Toru Ogata , Hirotsugu Tsuchimochi , Shinya Sato, Shigeyoshi Saito, Kohzoh Yoshino, Yuiko Matsuura , Kenichi Funamoto , Hiroki Ochi, Masahiro Shinohara, Motoshi Nagao & Yasuhiro Sawada

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: Compound Sakura Finetek Cat# 4583 Vectashield Mounting Medium with DAPI Vector Laboratories Cat# H-1200-10 Critical commercial assays Jo urn al Pr e-p roo f Phosphorylated Neurofilament H Human ELISA BioVendor Cat# RD191138300R Corticosterone EIA IDS Cat# AC-14F1 BCA Protein Assay Kit Pierce Cat# 23225 Deposited data Raw data This paper https://doi.org/10.3494 5/F52P4M Experimental models: Organisms/strains Sprague-Dawley Rat Charles River Laboratories RRID: MGI: 5651135 Software and algorithms Kinema Tracer System Kissei Comtec http://www.kinematrac er.com/ Odyssey Application Software Version 3.0 LI-COR Biosciences RRID: SCR_014579 NIS Elements Imaging Software Nikon Instruments RRID: SCR_014329; https://calm.ucsf.edu/h ardware-and-softwareimage-analysis ImageJ NIH RRID: SCR_003070 AutoQuant Media Cybernetics RRID: SCR_002465 MetaMorph Automation and Image Analysis Software Molecular Devices RRID: SCR_002368 Adobe Photoshop Adobe RRID: SCR_014199 SPSS Statistics version 26.0 IBM RRID: SCR_016479 Graphpad Prism Graphpad RRID: SCR_002798 Adobe Illustrator Adobe RRID: SCR_010279 Other Infinite Horizon Impactor Precision Systems and Instrumentation IH-0400; https://psiimpactors.co m/product/ih400/ Treadmill Muromachi Kikai MK-680; https://muromachi.com /en/archives/english/19 36/ Biological Amplifier Nihon Kohden NEC Biotop 6R12; https://doi.org/10.1371/ journal.pone.0171937 AD Converter Cambridge Electronic Design Limited Micro 1401; https://ced.co.uk/produ cts/microwaveformio Constant Current Stimulator Digitimer DS7A; https://www.digitimer.c om/product/humanneurophysiology/periph eral-stimulators/ds7ads7ah-hv-currentstimulator/ Jo urn al P e-p roo f Plate Reader Tecan GENios; https://www.tecan.com/ customer-news/geniosan-innovativedetection-device-thatdelivers-flexibility-tothe-molecular-biologylaboratory-2347 Infrared Imaging System LI-COR Biosciences Odyssey Infrared Imaging System; RRID: SCR_023765; https://doi.org/10.1038/ s41598-024-70096-0 Cryostat Leica Biosystems CM1950; RRID: SCR_018061; https://www.leicabiosys tems.com/us/histologyequipment/cryostats/lei ca-cm1950/ Inverted Microscope Nikon Instruments ECLIPSE Ti inverted microscope system; RRID: SCR_021242; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Spinning Disk Confocal Microscope Yokogawa Electric Corporation CSU-22; RRID: SCR_020907; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Jo urn al Pr e-p roo f

    Article Title: Atp13a5 Marker Reveals Pericyte Specification in the Mouse Central Nervous System
    Article Snippet: Rabbit polyclonal anti-NeuN , Millipore Sigma , ABN78.

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: For assessment of lesion pathology in the experimental animal model, the tissue sections of the thoracic spinal cord segments were incubated with Jo urn al Pr e-p ro f 58 diluted primary antibodies: rabbit polyclonal anti-NeuN (1:500; Millipore; Cat# ABN78; RRID: AB_10807945), mouse monoclonal anti-GFAP (1:500; Millipore; Cat# MAB360; RRID: AB_11212597), rabbit monoclonal anti-Vimentin (1:50; Cell Signaling Technology; Cat# 5741; RRID: AB_10695459), and rabbit polyclonal anti-GAP43 (1:500; Millipore; Cat# AB5220; RRID: AB_2107282).

    Article Title: SETD4 cells contribute to brain development and maintain adult stem cell reservoir for neurogenesis
    Article Snippet: The antibodies used in this study included mouse monoclonal anti-SETD4 (1:100, #sc-514060, Santa Cruz Biotechnology), rabbit polyclonal anti-GFAP (1:1000, #Z0334, Dako), goat polyclonal anti-GFAP (1:1000, #ab53554, Abcam), chicken polyclonal anti-NESTIN (1:1000, #NB100-1604, Novus Biologicals), mouse monoclonal anti-NESTIN (1:500, #MAB353, MilliporeSigma), rabbit polyclonal anti-SOX2 (1:200, #ab97959, Abcam), goat polyclonal anti-SOX2 (1:200, #AF2018, R&D Systems), rabbit monoclonal anti-KI67 (1:200, #ab16667, Abcam), mouse monoclonal antiPCNA (1:500, #ab29, Abcam), chicken polyclonal anti-GFP (1:1000, #ab13970, Abcam), rat monoclonal anti-BrdU (1:200, #ab6326, Abcam), goat polyclonal anti-DCX (1:500, #ab113435, Abcam), rabbit polyclonal anti-OLIG2 (1:500, #AB9610, MilliporeSigma), rabbit polyclonal anti-NEUN (1:1000, #ABN78, MilliporeSigma), mouse monoclonal antiMASH1 (1:200, #556604, BD Biosciences), chicken polyclonal anti-RFP (1:500, #600-901-379, Rockland immunochemicals), rabbit polyclonal anti-H4K20ME3 (1:500, #pAb-057-050, Diagenode), rabbit polyclonal antiH3K9AC (1:500, #ab10812, Abcam).

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: Rabbit Polyclonal Anti-NeuN , Millipore , Cat# ABN78; RRID: AB_10807945.

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: For assessment of lesion pathology in the experimental animal model, the tissue sections of the thoracic spinal cord segments were incubated with diluted primary antibodies: rabbit polyclonal anti-NeuN (1:500; Millipore; Cat# ABN78; RRID: AB_10807945 ), mouse monoclonal anti-GFAP (1:500; Millipore; Cat# MAB360; RRID: AB_11212597 ), rabbit monoclonal anti-Vimentin (1:50; Cell Signaling Technology; Cat# 5741; RRID: AB_10695459 ), and rabbit polyclonal anti-GAP43 (1:500; Millipore; Cat# AB5220; RRID: AB_2107282 ).

    Quantitative RT-PCR:

    Article Title: SETD4 cells contribute to brain development and maintain adult stem cell reservoir for neurogenesis
    Article Snippet: The antibodies used in this study included mouse monoclonal anti-SETD4 (1:100, #sc-514060, Santa Cruz Biotechnology), rabbit polyclonal anti-GFAP (1:1000, #Z0334, Dako), goat polyclonal anti-GFAP (1:1000, #ab53554, Abcam), chicken polyclonal anti-NESTIN (1:1000, #NB100-1604, Novus Biologicals), mouse monoclonal anti-NESTIN (1:500, #MAB353, MilliporeSigma), rabbit polyclonal anti-SOX2 (1:200, #ab97959, Abcam), goat polyclonal anti-SOX2 (1:200, #AF2018, R&D Systems), rabbit monoclonal anti-KI67 (1:200, #ab16667, Abcam), mouse monoclonal antiPCNA (1:500, #ab29, Abcam), chicken polyclonal anti-GFP (1:1000, #ab13970, Abcam), rat monoclonal anti-BrdU (1:200, #ab6326, Abcam), goat polyclonal anti-DCX (1:500, #ab113435, Abcam), rabbit polyclonal anti-OLIG2 (1:500, #AB9610, MilliporeSigma), rabbit polyclonal anti-NEUN (1:1000, #ABN78, MilliporeSigma), mouse monoclonal antiMASH1 (1:200, #556604, BD Biosciences), chicken polyclonal anti-RFP (1:500, #600-901-379, Rockland immunochemicals), rabbit polyclonal anti-H4K20ME3 (1:500, #pAb-057-050, Diagenode), rabbit polyclonal antiH3K9AC (1:500, #ab10812, Abcam).

    Article Title: Interstitial-fluid shear stresses induced by vertically oscillating head motion lower blood pressure in hypertensive rats and humans.
    Article Snippet: Shuhei Murase, Naoyoshi Sakitani, Takahiro Maekawa, Daisuke Yoshino , Kouji Takano, Ayumu Konno , Hirokazu Hirai , Taku Saito , Sakae Tanaka, Keisuke Shinohara, Takuya Kishi, Yuki Yoshikawa , Takamasa Sakai , Makoto Ayaori, Hirohiko Inanami, Koji Tomiyasu, Atsushi Takashima, Toru Ogata , Hirotsugu Tsuchimochi , Shinya Sato, Shigeyoshi Saito, Kohzoh Yoshino, Yuiko Matsuura , Kenichi Funamoto , Hiroki Ochi, Masahiro Shinohara, Motoshi Nagao & Yasuhiro Sawada

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: Compound Sakura Finetek Cat# 4583 Vectashield Mounting Medium with DAPI Vector Laboratories Cat# H-1200-10 Critical commercial assays Jo urn al Pr e-p roo f Phosphorylated Neurofilament H Human ELISA BioVendor Cat# RD191138300R Corticosterone EIA IDS Cat# AC-14F1 BCA Protein Assay Kit Pierce Cat# 23225 Deposited data Raw data This paper https://doi.org/10.3494 5/F52P4M Experimental models: Organisms/strains Sprague-Dawley Rat Charles River Laboratories RRID: MGI: 5651135 Software and algorithms Kinema Tracer System Kissei Comtec http://www.kinematrac er.com/ Odyssey Application Software Version 3.0 LI-COR Biosciences RRID: SCR_014579 NIS Elements Imaging Software Nikon Instruments RRID: SCR_014329; https://calm.ucsf.edu/h ardware-and-softwareimage-analysis ImageJ NIH RRID: SCR_003070 AutoQuant Media Cybernetics RRID: SCR_002465 MetaMorph Automation and Image Analysis Software Molecular Devices RRID: SCR_002368 Adobe Photoshop Adobe RRID: SCR_014199 SPSS Statistics version 26.0 IBM RRID: SCR_016479 Graphpad Prism Graphpad RRID: SCR_002798 Adobe Illustrator Adobe RRID: SCR_010279 Other Infinite Horizon Impactor Precision Systems and Instrumentation IH-0400; https://psiimpactors.co m/product/ih400/ Treadmill Muromachi Kikai MK-680; https://muromachi.com /en/archives/english/19 36/ Biological Amplifier Nihon Kohden NEC Biotop 6R12; https://doi.org/10.1371/ journal.pone.0171937 AD Converter Cambridge Electronic Design Limited Micro 1401; https://ced.co.uk/produ cts/microwaveformio Constant Current Stimulator Digitimer DS7A; https://www.digitimer.c om/product/humanneurophysiology/periph eral-stimulators/ds7ads7ah-hv-currentstimulator/ Jo urn al P e-p roo f Plate Reader Tecan GENios; https://www.tecan.com/ customer-news/geniosan-innovativedetection-device-thatdelivers-flexibility-tothe-molecular-biologylaboratory-2347 Infrared Imaging System LI-COR Biosciences Odyssey Infrared Imaging System; RRID: SCR_023765; https://doi.org/10.1038/ s41598-024-70096-0 Cryostat Leica Biosystems CM1950; RRID: SCR_018061; https://www.leicabiosys tems.com/us/histologyequipment/cryostats/lei ca-cm1950/ Inverted Microscope Nikon Instruments ECLIPSE Ti inverted microscope system; RRID: SCR_021242; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Spinning Disk Confocal Microscope Yokogawa Electric Corporation CSU-22; RRID: SCR_020907; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Jo urn al Pr e-p roo f

    Article Title: Atp13a5 Marker Reveals Pericyte Specification in the Mouse Central Nervous System
    Article Snippet: Rabbit polyclonal anti-NeuN , Millipore Sigma , ABN78.

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: For assessment of lesion pathology in the experimental animal model, the tissue sections of the thoracic spinal cord segments were incubated with Jo urn al Pr e-p ro f 58 diluted primary antibodies: rabbit polyclonal anti-NeuN (1:500; Millipore; Cat# ABN78; RRID: AB_10807945), mouse monoclonal anti-GFAP (1:500; Millipore; Cat# MAB360; RRID: AB_11212597), rabbit monoclonal anti-Vimentin (1:50; Cell Signaling Technology; Cat# 5741; RRID: AB_10695459), and rabbit polyclonal anti-GAP43 (1:500; Millipore; Cat# AB5220; RRID: AB_2107282).

    Article Title: SETD4 cells contribute to brain development and maintain adult stem cell reservoir for neurogenesis
    Article Snippet: The antibodies used in this study included mouse monoclonal anti-SETD4 (1:100, #sc-514060, Santa Cruz Biotechnology), rabbit polyclonal anti-GFAP (1:1000, #Z0334, Dako), goat polyclonal anti-GFAP (1:1000, #ab53554, Abcam), chicken polyclonal anti-NESTIN (1:1000, #NB100-1604, Novus Biologicals), mouse monoclonal anti-NESTIN (1:500, #MAB353, MilliporeSigma), rabbit polyclonal anti-SOX2 (1:200, #ab97959, Abcam), goat polyclonal anti-SOX2 (1:200, #AF2018, R&D Systems), rabbit monoclonal anti-KI67 (1:200, #ab16667, Abcam), mouse monoclonal antiPCNA (1:500, #ab29, Abcam), chicken polyclonal anti-GFP (1:1000, #ab13970, Abcam), rat monoclonal anti-BrdU (1:200, #ab6326, Abcam), goat polyclonal anti-DCX (1:500, #ab113435, Abcam), rabbit polyclonal anti-OLIG2 (1:500, #AB9610, MilliporeSigma), rabbit polyclonal anti-NEUN (1:1000, #ABN78, MilliporeSigma), mouse monoclonal antiMASH1 (1:200, #556604, BD Biosciences), chicken polyclonal anti-RFP (1:500, #600-901-379, Rockland immunochemicals), rabbit polyclonal anti-H4K20ME3 (1:500, #pAb-057-050, Diagenode), rabbit polyclonal antiH3K9AC (1:500, #ab10812, Abcam).

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: Rabbit Polyclonal Anti-NeuN , Millipore , Cat# ABN78; RRID: AB_10807945.

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: For assessment of lesion pathology in the experimental animal model, the tissue sections of the thoracic spinal cord segments were incubated with diluted primary antibodies: rabbit polyclonal anti-NeuN (1:500; Millipore; Cat# ABN78; RRID: AB_10807945 ), mouse monoclonal anti-GFAP (1:500; Millipore; Cat# MAB360; RRID: AB_11212597 ), rabbit monoclonal anti-Vimentin (1:50; Cell Signaling Technology; Cat# 5741; RRID: AB_10695459 ), and rabbit polyclonal anti-GAP43 (1:500; Millipore; Cat# AB5220; RRID: AB_2107282 ).

    SYBR Green Assay:

    Article Title: SETD4 cells contribute to brain development and maintain adult stem cell reservoir for neurogenesis
    Article Snippet: The antibodies used in this study included mouse monoclonal anti-SETD4 (1:100, #sc-514060, Santa Cruz Biotechnology), rabbit polyclonal anti-GFAP (1:1000, #Z0334, Dako), goat polyclonal anti-GFAP (1:1000, #ab53554, Abcam), chicken polyclonal anti-NESTIN (1:1000, #NB100-1604, Novus Biologicals), mouse monoclonal anti-NESTIN (1:500, #MAB353, MilliporeSigma), rabbit polyclonal anti-SOX2 (1:200, #ab97959, Abcam), goat polyclonal anti-SOX2 (1:200, #AF2018, R&D Systems), rabbit monoclonal anti-KI67 (1:200, #ab16667, Abcam), mouse monoclonal antiPCNA (1:500, #ab29, Abcam), chicken polyclonal anti-GFP (1:1000, #ab13970, Abcam), rat monoclonal anti-BrdU (1:200, #ab6326, Abcam), goat polyclonal anti-DCX (1:500, #ab113435, Abcam), rabbit polyclonal anti-OLIG2 (1:500, #AB9610, MilliporeSigma), rabbit polyclonal anti-NEUN (1:1000, #ABN78, MilliporeSigma), mouse monoclonal antiMASH1 (1:200, #556604, BD Biosciences), chicken polyclonal anti-RFP (1:500, #600-901-379, Rockland immunochemicals), rabbit polyclonal anti-H4K20ME3 (1:500, #pAb-057-050, Diagenode), rabbit polyclonal antiH3K9AC (1:500, #ab10812, Abcam).

    Article Title: Interstitial-fluid shear stresses induced by vertically oscillating head motion lower blood pressure in hypertensive rats and humans.
    Article Snippet: Shuhei Murase, Naoyoshi Sakitani, Takahiro Maekawa, Daisuke Yoshino , Kouji Takano, Ayumu Konno , Hirokazu Hirai , Taku Saito , Sakae Tanaka, Keisuke Shinohara, Takuya Kishi, Yuki Yoshikawa , Takamasa Sakai , Makoto Ayaori, Hirohiko Inanami, Koji Tomiyasu, Atsushi Takashima, Toru Ogata , Hirotsugu Tsuchimochi , Shinya Sato, Shigeyoshi Saito, Kohzoh Yoshino, Yuiko Matsuura , Kenichi Funamoto , Hiroki Ochi, Masahiro Shinohara, Motoshi Nagao & Yasuhiro Sawada

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: Compound Sakura Finetek Cat# 4583 Vectashield Mounting Medium with DAPI Vector Laboratories Cat# H-1200-10 Critical commercial assays Jo urn al Pr e-p roo f Phosphorylated Neurofilament H Human ELISA BioVendor Cat# RD191138300R Corticosterone EIA IDS Cat# AC-14F1 BCA Protein Assay Kit Pierce Cat# 23225 Deposited data Raw data This paper https://doi.org/10.3494 5/F52P4M Experimental models: Organisms/strains Sprague-Dawley Rat Charles River Laboratories RRID: MGI: 5651135 Software and algorithms Kinema Tracer System Kissei Comtec http://www.kinematrac er.com/ Odyssey Application Software Version 3.0 LI-COR Biosciences RRID: SCR_014579 NIS Elements Imaging Software Nikon Instruments RRID: SCR_014329; https://calm.ucsf.edu/h ardware-and-softwareimage-analysis ImageJ NIH RRID: SCR_003070 AutoQuant Media Cybernetics RRID: SCR_002465 MetaMorph Automation and Image Analysis Software Molecular Devices RRID: SCR_002368 Adobe Photoshop Adobe RRID: SCR_014199 SPSS Statistics version 26.0 IBM RRID: SCR_016479 Graphpad Prism Graphpad RRID: SCR_002798 Adobe Illustrator Adobe RRID: SCR_010279 Other Infinite Horizon Impactor Precision Systems and Instrumentation IH-0400; https://psiimpactors.co m/product/ih400/ Treadmill Muromachi Kikai MK-680; https://muromachi.com /en/archives/english/19 36/ Biological Amplifier Nihon Kohden NEC Biotop 6R12; https://doi.org/10.1371/ journal.pone.0171937 AD Converter Cambridge Electronic Design Limited Micro 1401; https://ced.co.uk/produ cts/microwaveformio Constant Current Stimulator Digitimer DS7A; https://www.digitimer.c om/product/humanneurophysiology/periph eral-stimulators/ds7ads7ah-hv-currentstimulator/ Jo urn al P e-p roo f Plate Reader Tecan GENios; https://www.tecan.com/ customer-news/geniosan-innovativedetection-device-thatdelivers-flexibility-tothe-molecular-biologylaboratory-2347 Infrared Imaging System LI-COR Biosciences Odyssey Infrared Imaging System; RRID: SCR_023765; https://doi.org/10.1038/ s41598-024-70096-0 Cryostat Leica Biosystems CM1950; RRID: SCR_018061; https://www.leicabiosys tems.com/us/histologyequipment/cryostats/lei ca-cm1950/ Inverted Microscope Nikon Instruments ECLIPSE Ti inverted microscope system; RRID: SCR_021242; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Spinning Disk Confocal Microscope Yokogawa Electric Corporation CSU-22; RRID: SCR_020907; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Jo urn al Pr e-p roo f

    Article Title: Atp13a5 Marker Reveals Pericyte Specification in the Mouse Central Nervous System
    Article Snippet: Rabbit polyclonal anti-NeuN , Millipore Sigma , ABN78.

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: For assessment of lesion pathology in the experimental animal model, the tissue sections of the thoracic spinal cord segments were incubated with Jo urn al Pr e-p ro f 58 diluted primary antibodies: rabbit polyclonal anti-NeuN (1:500; Millipore; Cat# ABN78; RRID: AB_10807945), mouse monoclonal anti-GFAP (1:500; Millipore; Cat# MAB360; RRID: AB_11212597), rabbit monoclonal anti-Vimentin (1:50; Cell Signaling Technology; Cat# 5741; RRID: AB_10695459), and rabbit polyclonal anti-GAP43 (1:500; Millipore; Cat# AB5220; RRID: AB_2107282).

    Article Title: SETD4 cells contribute to brain development and maintain adult stem cell reservoir for neurogenesis
    Article Snippet: The antibodies used in this study included mouse monoclonal anti-SETD4 (1:100, #sc-514060, Santa Cruz Biotechnology), rabbit polyclonal anti-GFAP (1:1000, #Z0334, Dako), goat polyclonal anti-GFAP (1:1000, #ab53554, Abcam), chicken polyclonal anti-NESTIN (1:1000, #NB100-1604, Novus Biologicals), mouse monoclonal anti-NESTIN (1:500, #MAB353, MilliporeSigma), rabbit polyclonal anti-SOX2 (1:200, #ab97959, Abcam), goat polyclonal anti-SOX2 (1:200, #AF2018, R&D Systems), rabbit monoclonal anti-KI67 (1:200, #ab16667, Abcam), mouse monoclonal antiPCNA (1:500, #ab29, Abcam), chicken polyclonal anti-GFP (1:1000, #ab13970, Abcam), rat monoclonal anti-BrdU (1:200, #ab6326, Abcam), goat polyclonal anti-DCX (1:500, #ab113435, Abcam), rabbit polyclonal anti-OLIG2 (1:500, #AB9610, MilliporeSigma), rabbit polyclonal anti-NEUN (1:1000, #ABN78, MilliporeSigma), mouse monoclonal antiMASH1 (1:200, #556604, BD Biosciences), chicken polyclonal anti-RFP (1:500, #600-901-379, Rockland immunochemicals), rabbit polyclonal anti-H4K20ME3 (1:500, #pAb-057-050, Diagenode), rabbit polyclonal antiH3K9AC (1:500, #ab10812, Abcam).

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: Rabbit Polyclonal Anti-NeuN , Millipore , Cat# ABN78; RRID: AB_10807945.

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: For assessment of lesion pathology in the experimental animal model, the tissue sections of the thoracic spinal cord segments were incubated with diluted primary antibodies: rabbit polyclonal anti-NeuN (1:500; Millipore; Cat# ABN78; RRID: AB_10807945 ), mouse monoclonal anti-GFAP (1:500; Millipore; Cat# MAB360; RRID: AB_11212597 ), rabbit monoclonal anti-Vimentin (1:50; Cell Signaling Technology; Cat# 5741; RRID: AB_10695459 ), and rabbit polyclonal anti-GAP43 (1:500; Millipore; Cat# AB5220; RRID: AB_2107282 ).

    Immunohistochemistry:

    Article Title: SETD4 cells contribute to brain development and maintain adult stem cell reservoir for neurogenesis
    Article Snippet: The antibodies used in this study included mouse monoclonal anti-SETD4 (1:100, #sc-514060, Santa Cruz Biotechnology), rabbit polyclonal anti-GFAP (1:1000, #Z0334, Dako), goat polyclonal anti-GFAP (1:1000, #ab53554, Abcam), chicken polyclonal anti-NESTIN (1:1000, #NB100-1604, Novus Biologicals), mouse monoclonal anti-NESTIN (1:500, #MAB353, MilliporeSigma), rabbit polyclonal anti-SOX2 (1:200, #ab97959, Abcam), goat polyclonal anti-SOX2 (1:200, #AF2018, R&D Systems), rabbit monoclonal anti-KI67 (1:200, #ab16667, Abcam), mouse monoclonal antiPCNA (1:500, #ab29, Abcam), chicken polyclonal anti-GFP (1:1000, #ab13970, Abcam), rat monoclonal anti-BrdU (1:200, #ab6326, Abcam), goat polyclonal anti-DCX (1:500, #ab113435, Abcam), rabbit polyclonal anti-OLIG2 (1:500, #AB9610, MilliporeSigma), rabbit polyclonal anti-NEUN (1:1000, #ABN78, MilliporeSigma), mouse monoclonal antiMASH1 (1:200, #556604, BD Biosciences), chicken polyclonal anti-RFP (1:500, #600-901-379, Rockland immunochemicals), rabbit polyclonal anti-H4K20ME3 (1:500, #pAb-057-050, Diagenode), rabbit polyclonal antiH3K9AC (1:500, #ab10812, Abcam).

    Article Title: Interstitial-fluid shear stresses induced by vertically oscillating head motion lower blood pressure in hypertensive rats and humans.
    Article Snippet: Shuhei Murase, Naoyoshi Sakitani, Takahiro Maekawa, Daisuke Yoshino , Kouji Takano, Ayumu Konno , Hirokazu Hirai , Taku Saito , Sakae Tanaka, Keisuke Shinohara, Takuya Kishi, Yuki Yoshikawa , Takamasa Sakai , Makoto Ayaori, Hirohiko Inanami, Koji Tomiyasu, Atsushi Takashima, Toru Ogata , Hirotsugu Tsuchimochi , Shinya Sato, Shigeyoshi Saito, Kohzoh Yoshino, Yuiko Matsuura , Kenichi Funamoto , Hiroki Ochi, Masahiro Shinohara, Motoshi Nagao & Yasuhiro Sawada

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: Compound Sakura Finetek Cat# 4583 Vectashield Mounting Medium with DAPI Vector Laboratories Cat# H-1200-10 Critical commercial assays Jo urn al Pr e-p roo f Phosphorylated Neurofilament H Human ELISA BioVendor Cat# RD191138300R Corticosterone EIA IDS Cat# AC-14F1 BCA Protein Assay Kit Pierce Cat# 23225 Deposited data Raw data This paper https://doi.org/10.3494 5/F52P4M Experimental models: Organisms/strains Sprague-Dawley Rat Charles River Laboratories RRID: MGI: 5651135 Software and algorithms Kinema Tracer System Kissei Comtec http://www.kinematrac er.com/ Odyssey Application Software Version 3.0 LI-COR Biosciences RRID: SCR_014579 NIS Elements Imaging Software Nikon Instruments RRID: SCR_014329; https://calm.ucsf.edu/h ardware-and-softwareimage-analysis ImageJ NIH RRID: SCR_003070 AutoQuant Media Cybernetics RRID: SCR_002465 MetaMorph Automation and Image Analysis Software Molecular Devices RRID: SCR_002368 Adobe Photoshop Adobe RRID: SCR_014199 SPSS Statistics version 26.0 IBM RRID: SCR_016479 Graphpad Prism Graphpad RRID: SCR_002798 Adobe Illustrator Adobe RRID: SCR_010279 Other Infinite Horizon Impactor Precision Systems and Instrumentation IH-0400; https://psiimpactors.co m/product/ih400/ Treadmill Muromachi Kikai MK-680; https://muromachi.com /en/archives/english/19 36/ Biological Amplifier Nihon Kohden NEC Biotop 6R12; https://doi.org/10.1371/ journal.pone.0171937 AD Converter Cambridge Electronic Design Limited Micro 1401; https://ced.co.uk/produ cts/microwaveformio Constant Current Stimulator Digitimer DS7A; https://www.digitimer.c om/product/humanneurophysiology/periph eral-stimulators/ds7ads7ah-hv-currentstimulator/ Jo urn al P e-p roo f Plate Reader Tecan GENios; https://www.tecan.com/ customer-news/geniosan-innovativedetection-device-thatdelivers-flexibility-tothe-molecular-biologylaboratory-2347 Infrared Imaging System LI-COR Biosciences Odyssey Infrared Imaging System; RRID: SCR_023765; https://doi.org/10.1038/ s41598-024-70096-0 Cryostat Leica Biosystems CM1950; RRID: SCR_018061; https://www.leicabiosys tems.com/us/histologyequipment/cryostats/lei ca-cm1950/ Inverted Microscope Nikon Instruments ECLIPSE Ti inverted microscope system; RRID: SCR_021242; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Spinning Disk Confocal Microscope Yokogawa Electric Corporation CSU-22; RRID: SCR_020907; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Jo urn al Pr e-p roo f

    Article Title: Atp13a5 Marker Reveals Pericyte Specification in the Mouse Central Nervous System
    Article Snippet: Rabbit polyclonal anti-NeuN , Millipore Sigma , ABN78.

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: For assessment of lesion pathology in the experimental animal model, the tissue sections of the thoracic spinal cord segments were incubated with Jo urn al Pr e-p ro f 58 diluted primary antibodies: rabbit polyclonal anti-NeuN (1:500; Millipore; Cat# ABN78; RRID: AB_10807945), mouse monoclonal anti-GFAP (1:500; Millipore; Cat# MAB360; RRID: AB_11212597), rabbit monoclonal anti-Vimentin (1:50; Cell Signaling Technology; Cat# 5741; RRID: AB_10695459), and rabbit polyclonal anti-GAP43 (1:500; Millipore; Cat# AB5220; RRID: AB_2107282).

    Article Title: SETD4 cells contribute to brain development and maintain adult stem cell reservoir for neurogenesis
    Article Snippet: The antibodies used in this study included mouse monoclonal anti-SETD4 (1:100, #sc-514060, Santa Cruz Biotechnology), rabbit polyclonal anti-GFAP (1:1000, #Z0334, Dako), goat polyclonal anti-GFAP (1:1000, #ab53554, Abcam), chicken polyclonal anti-NESTIN (1:1000, #NB100-1604, Novus Biologicals), mouse monoclonal anti-NESTIN (1:500, #MAB353, MilliporeSigma), rabbit polyclonal anti-SOX2 (1:200, #ab97959, Abcam), goat polyclonal anti-SOX2 (1:200, #AF2018, R&D Systems), rabbit monoclonal anti-KI67 (1:200, #ab16667, Abcam), mouse monoclonal antiPCNA (1:500, #ab29, Abcam), chicken polyclonal anti-GFP (1:1000, #ab13970, Abcam), rat monoclonal anti-BrdU (1:200, #ab6326, Abcam), goat polyclonal anti-DCX (1:500, #ab113435, Abcam), rabbit polyclonal anti-OLIG2 (1:500, #AB9610, MilliporeSigma), rabbit polyclonal anti-NEUN (1:1000, #ABN78, MilliporeSigma), mouse monoclonal antiMASH1 (1:200, #556604, BD Biosciences), chicken polyclonal anti-RFP (1:500, #600-901-379, Rockland immunochemicals), rabbit polyclonal anti-H4K20ME3 (1:500, #pAb-057-050, Diagenode), rabbit polyclonal antiH3K9AC (1:500, #ab10812, Abcam).

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: Rabbit Polyclonal Anti-NeuN , Millipore , Cat# ABN78; RRID: AB_10807945.

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: For assessment of lesion pathology in the experimental animal model, the tissue sections of the thoracic spinal cord segments were incubated with diluted primary antibodies: rabbit polyclonal anti-NeuN (1:500; Millipore; Cat# ABN78; RRID: AB_10807945 ), mouse monoclonal anti-GFAP (1:500; Millipore; Cat# MAB360; RRID: AB_11212597 ), rabbit monoclonal anti-Vimentin (1:50; Cell Signaling Technology; Cat# 5741; RRID: AB_10695459 ), and rabbit polyclonal anti-GAP43 (1:500; Millipore; Cat# AB5220; RRID: AB_2107282 ).

    In Situ Hybridization:

    Article Title: SETD4 cells contribute to brain development and maintain adult stem cell reservoir for neurogenesis
    Article Snippet: The antibodies used in this study included mouse monoclonal anti-SETD4 (1:100, #sc-514060, Santa Cruz Biotechnology), rabbit polyclonal anti-GFAP (1:1000, #Z0334, Dako), goat polyclonal anti-GFAP (1:1000, #ab53554, Abcam), chicken polyclonal anti-NESTIN (1:1000, #NB100-1604, Novus Biologicals), mouse monoclonal anti-NESTIN (1:500, #MAB353, MilliporeSigma), rabbit polyclonal anti-SOX2 (1:200, #ab97959, Abcam), goat polyclonal anti-SOX2 (1:200, #AF2018, R&D Systems), rabbit monoclonal anti-KI67 (1:200, #ab16667, Abcam), mouse monoclonal antiPCNA (1:500, #ab29, Abcam), chicken polyclonal anti-GFP (1:1000, #ab13970, Abcam), rat monoclonal anti-BrdU (1:200, #ab6326, Abcam), goat polyclonal anti-DCX (1:500, #ab113435, Abcam), rabbit polyclonal anti-OLIG2 (1:500, #AB9610, MilliporeSigma), rabbit polyclonal anti-NEUN (1:1000, #ABN78, MilliporeSigma), mouse monoclonal antiMASH1 (1:200, #556604, BD Biosciences), chicken polyclonal anti-RFP (1:500, #600-901-379, Rockland immunochemicals), rabbit polyclonal anti-H4K20ME3 (1:500, #pAb-057-050, Diagenode), rabbit polyclonal antiH3K9AC (1:500, #ab10812, Abcam).

    Article Title: Interstitial-fluid shear stresses induced by vertically oscillating head motion lower blood pressure in hypertensive rats and humans.
    Article Snippet: Shuhei Murase, Naoyoshi Sakitani, Takahiro Maekawa, Daisuke Yoshino , Kouji Takano, Ayumu Konno , Hirokazu Hirai , Taku Saito , Sakae Tanaka, Keisuke Shinohara, Takuya Kishi, Yuki Yoshikawa , Takamasa Sakai , Makoto Ayaori, Hirohiko Inanami, Koji Tomiyasu, Atsushi Takashima, Toru Ogata , Hirotsugu Tsuchimochi , Shinya Sato, Shigeyoshi Saito, Kohzoh Yoshino, Yuiko Matsuura , Kenichi Funamoto , Hiroki Ochi, Masahiro Shinohara, Motoshi Nagao & Yasuhiro Sawada

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: Compound Sakura Finetek Cat# 4583 Vectashield Mounting Medium with DAPI Vector Laboratories Cat# H-1200-10 Critical commercial assays Jo urn al Pr e-p roo f Phosphorylated Neurofilament H Human ELISA BioVendor Cat# RD191138300R Corticosterone EIA IDS Cat# AC-14F1 BCA Protein Assay Kit Pierce Cat# 23225 Deposited data Raw data This paper https://doi.org/10.3494 5/F52P4M Experimental models: Organisms/strains Sprague-Dawley Rat Charles River Laboratories RRID: MGI: 5651135 Software and algorithms Kinema Tracer System Kissei Comtec http://www.kinematrac er.com/ Odyssey Application Software Version 3.0 LI-COR Biosciences RRID: SCR_014579 NIS Elements Imaging Software Nikon Instruments RRID: SCR_014329; https://calm.ucsf.edu/h ardware-and-softwareimage-analysis ImageJ NIH RRID: SCR_003070 AutoQuant Media Cybernetics RRID: SCR_002465 MetaMorph Automation and Image Analysis Software Molecular Devices RRID: SCR_002368 Adobe Photoshop Adobe RRID: SCR_014199 SPSS Statistics version 26.0 IBM RRID: SCR_016479 Graphpad Prism Graphpad RRID: SCR_002798 Adobe Illustrator Adobe RRID: SCR_010279 Other Infinite Horizon Impactor Precision Systems and Instrumentation IH-0400; https://psiimpactors.co m/product/ih400/ Treadmill Muromachi Kikai MK-680; https://muromachi.com /en/archives/english/19 36/ Biological Amplifier Nihon Kohden NEC Biotop 6R12; https://doi.org/10.1371/ journal.pone.0171937 AD Converter Cambridge Electronic Design Limited Micro 1401; https://ced.co.uk/produ cts/microwaveformio Constant Current Stimulator Digitimer DS7A; https://www.digitimer.c om/product/humanneurophysiology/periph eral-stimulators/ds7ads7ah-hv-currentstimulator/ Jo urn al P e-p roo f Plate Reader Tecan GENios; https://www.tecan.com/ customer-news/geniosan-innovativedetection-device-thatdelivers-flexibility-tothe-molecular-biologylaboratory-2347 Infrared Imaging System LI-COR Biosciences Odyssey Infrared Imaging System; RRID: SCR_023765; https://doi.org/10.1038/ s41598-024-70096-0 Cryostat Leica Biosystems CM1950; RRID: SCR_018061; https://www.leicabiosys tems.com/us/histologyequipment/cryostats/lei ca-cm1950/ Inverted Microscope Nikon Instruments ECLIPSE Ti inverted microscope system; RRID: SCR_021242; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Spinning Disk Confocal Microscope Yokogawa Electric Corporation CSU-22; RRID: SCR_020907; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Jo urn al Pr e-p roo f

    Article Title: Atp13a5 Marker Reveals Pericyte Specification in the Mouse Central Nervous System
    Article Snippet: Rabbit polyclonal anti-NeuN , Millipore Sigma , ABN78.

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: For assessment of lesion pathology in the experimental animal model, the tissue sections of the thoracic spinal cord segments were incubated with Jo urn al Pr e-p ro f 58 diluted primary antibodies: rabbit polyclonal anti-NeuN (1:500; Millipore; Cat# ABN78; RRID: AB_10807945), mouse monoclonal anti-GFAP (1:500; Millipore; Cat# MAB360; RRID: AB_11212597), rabbit monoclonal anti-Vimentin (1:50; Cell Signaling Technology; Cat# 5741; RRID: AB_10695459), and rabbit polyclonal anti-GAP43 (1:500; Millipore; Cat# AB5220; RRID: AB_2107282).

    Article Title: SETD4 cells contribute to brain development and maintain adult stem cell reservoir for neurogenesis
    Article Snippet: The antibodies used in this study included mouse monoclonal anti-SETD4 (1:100, #sc-514060, Santa Cruz Biotechnology), rabbit polyclonal anti-GFAP (1:1000, #Z0334, Dako), goat polyclonal anti-GFAP (1:1000, #ab53554, Abcam), chicken polyclonal anti-NESTIN (1:1000, #NB100-1604, Novus Biologicals), mouse monoclonal anti-NESTIN (1:500, #MAB353, MilliporeSigma), rabbit polyclonal anti-SOX2 (1:200, #ab97959, Abcam), goat polyclonal anti-SOX2 (1:200, #AF2018, R&D Systems), rabbit monoclonal anti-KI67 (1:200, #ab16667, Abcam), mouse monoclonal antiPCNA (1:500, #ab29, Abcam), chicken polyclonal anti-GFP (1:1000, #ab13970, Abcam), rat monoclonal anti-BrdU (1:200, #ab6326, Abcam), goat polyclonal anti-DCX (1:500, #ab113435, Abcam), rabbit polyclonal anti-OLIG2 (1:500, #AB9610, MilliporeSigma), rabbit polyclonal anti-NEUN (1:1000, #ABN78, MilliporeSigma), mouse monoclonal antiMASH1 (1:200, #556604, BD Biosciences), chicken polyclonal anti-RFP (1:500, #600-901-379, Rockland immunochemicals), rabbit polyclonal anti-H4K20ME3 (1:500, #pAb-057-050, Diagenode), rabbit polyclonal antiH3K9AC (1:500, #ab10812, Abcam).

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: Rabbit Polyclonal Anti-NeuN , Millipore , Cat# ABN78; RRID: AB_10807945.

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: For assessment of lesion pathology in the experimental animal model, the tissue sections of the thoracic spinal cord segments were incubated with diluted primary antibodies: rabbit polyclonal anti-NeuN (1:500; Millipore; Cat# ABN78; RRID: AB_10807945 ), mouse monoclonal anti-GFAP (1:500; Millipore; Cat# MAB360; RRID: AB_11212597 ), rabbit monoclonal anti-Vimentin (1:50; Cell Signaling Technology; Cat# 5741; RRID: AB_10695459 ), and rabbit polyclonal anti-GAP43 (1:500; Millipore; Cat# AB5220; RRID: AB_2107282 ).

    RNAscope:

    Article Title: SETD4 cells contribute to brain development and maintain adult stem cell reservoir for neurogenesis
    Article Snippet: The antibodies used in this study included mouse monoclonal anti-SETD4 (1:100, #sc-514060, Santa Cruz Biotechnology), rabbit polyclonal anti-GFAP (1:1000, #Z0334, Dako), goat polyclonal anti-GFAP (1:1000, #ab53554, Abcam), chicken polyclonal anti-NESTIN (1:1000, #NB100-1604, Novus Biologicals), mouse monoclonal anti-NESTIN (1:500, #MAB353, MilliporeSigma), rabbit polyclonal anti-SOX2 (1:200, #ab97959, Abcam), goat polyclonal anti-SOX2 (1:200, #AF2018, R&D Systems), rabbit monoclonal anti-KI67 (1:200, #ab16667, Abcam), mouse monoclonal antiPCNA (1:500, #ab29, Abcam), chicken polyclonal anti-GFP (1:1000, #ab13970, Abcam), rat monoclonal anti-BrdU (1:200, #ab6326, Abcam), goat polyclonal anti-DCX (1:500, #ab113435, Abcam), rabbit polyclonal anti-OLIG2 (1:500, #AB9610, MilliporeSigma), rabbit polyclonal anti-NEUN (1:1000, #ABN78, MilliporeSigma), mouse monoclonal antiMASH1 (1:200, #556604, BD Biosciences), chicken polyclonal anti-RFP (1:500, #600-901-379, Rockland immunochemicals), rabbit polyclonal anti-H4K20ME3 (1:500, #pAb-057-050, Diagenode), rabbit polyclonal antiH3K9AC (1:500, #ab10812, Abcam).

    Article Title: Interstitial-fluid shear stresses induced by vertically oscillating head motion lower blood pressure in hypertensive rats and humans.
    Article Snippet: Shuhei Murase, Naoyoshi Sakitani, Takahiro Maekawa, Daisuke Yoshino , Kouji Takano, Ayumu Konno , Hirokazu Hirai , Taku Saito , Sakae Tanaka, Keisuke Shinohara, Takuya Kishi, Yuki Yoshikawa , Takamasa Sakai , Makoto Ayaori, Hirohiko Inanami, Koji Tomiyasu, Atsushi Takashima, Toru Ogata , Hirotsugu Tsuchimochi , Shinya Sato, Shigeyoshi Saito, Kohzoh Yoshino, Yuiko Matsuura , Kenichi Funamoto , Hiroki Ochi, Masahiro Shinohara, Motoshi Nagao & Yasuhiro Sawada

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: Compound Sakura Finetek Cat# 4583 Vectashield Mounting Medium with DAPI Vector Laboratories Cat# H-1200-10 Critical commercial assays Jo urn al Pr e-p roo f Phosphorylated Neurofilament H Human ELISA BioVendor Cat# RD191138300R Corticosterone EIA IDS Cat# AC-14F1 BCA Protein Assay Kit Pierce Cat# 23225 Deposited data Raw data This paper https://doi.org/10.3494 5/F52P4M Experimental models: Organisms/strains Sprague-Dawley Rat Charles River Laboratories RRID: MGI: 5651135 Software and algorithms Kinema Tracer System Kissei Comtec http://www.kinematrac er.com/ Odyssey Application Software Version 3.0 LI-COR Biosciences RRID: SCR_014579 NIS Elements Imaging Software Nikon Instruments RRID: SCR_014329; https://calm.ucsf.edu/h ardware-and-softwareimage-analysis ImageJ NIH RRID: SCR_003070 AutoQuant Media Cybernetics RRID: SCR_002465 MetaMorph Automation and Image Analysis Software Molecular Devices RRID: SCR_002368 Adobe Photoshop Adobe RRID: SCR_014199 SPSS Statistics version 26.0 IBM RRID: SCR_016479 Graphpad Prism Graphpad RRID: SCR_002798 Adobe Illustrator Adobe RRID: SCR_010279 Other Infinite Horizon Impactor Precision Systems and Instrumentation IH-0400; https://psiimpactors.co m/product/ih400/ Treadmill Muromachi Kikai MK-680; https://muromachi.com /en/archives/english/19 36/ Biological Amplifier Nihon Kohden NEC Biotop 6R12; https://doi.org/10.1371/ journal.pone.0171937 AD Converter Cambridge Electronic Design Limited Micro 1401; https://ced.co.uk/produ cts/microwaveformio Constant Current Stimulator Digitimer DS7A; https://www.digitimer.c om/product/humanneurophysiology/periph eral-stimulators/ds7ads7ah-hv-currentstimulator/ Jo urn al P e-p roo f Plate Reader Tecan GENios; https://www.tecan.com/ customer-news/geniosan-innovativedetection-device-thatdelivers-flexibility-tothe-molecular-biologylaboratory-2347 Infrared Imaging System LI-COR Biosciences Odyssey Infrared Imaging System; RRID: SCR_023765; https://doi.org/10.1038/ s41598-024-70096-0 Cryostat Leica Biosystems CM1950; RRID: SCR_018061; https://www.leicabiosys tems.com/us/histologyequipment/cryostats/lei ca-cm1950/ Inverted Microscope Nikon Instruments ECLIPSE Ti inverted microscope system; RRID: SCR_021242; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Spinning Disk Confocal Microscope Yokogawa Electric Corporation CSU-22; RRID: SCR_020907; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Jo urn al Pr e-p roo f

    Article Title: Atp13a5 Marker Reveals Pericyte Specification in the Mouse Central Nervous System
    Article Snippet: Rabbit polyclonal anti-NeuN , Millipore Sigma , ABN78.

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: For assessment of lesion pathology in the experimental animal model, the tissue sections of the thoracic spinal cord segments were incubated with Jo urn al Pr e-p ro f 58 diluted primary antibodies: rabbit polyclonal anti-NeuN (1:500; Millipore; Cat# ABN78; RRID: AB_10807945), mouse monoclonal anti-GFAP (1:500; Millipore; Cat# MAB360; RRID: AB_11212597), rabbit monoclonal anti-Vimentin (1:50; Cell Signaling Technology; Cat# 5741; RRID: AB_10695459), and rabbit polyclonal anti-GAP43 (1:500; Millipore; Cat# AB5220; RRID: AB_2107282).

    Article Title: SETD4 cells contribute to brain development and maintain adult stem cell reservoir for neurogenesis
    Article Snippet: The antibodies used in this study included mouse monoclonal anti-SETD4 (1:100, #sc-514060, Santa Cruz Biotechnology), rabbit polyclonal anti-GFAP (1:1000, #Z0334, Dako), goat polyclonal anti-GFAP (1:1000, #ab53554, Abcam), chicken polyclonal anti-NESTIN (1:1000, #NB100-1604, Novus Biologicals), mouse monoclonal anti-NESTIN (1:500, #MAB353, MilliporeSigma), rabbit polyclonal anti-SOX2 (1:200, #ab97959, Abcam), goat polyclonal anti-SOX2 (1:200, #AF2018, R&D Systems), rabbit monoclonal anti-KI67 (1:200, #ab16667, Abcam), mouse monoclonal antiPCNA (1:500, #ab29, Abcam), chicken polyclonal anti-GFP (1:1000, #ab13970, Abcam), rat monoclonal anti-BrdU (1:200, #ab6326, Abcam), goat polyclonal anti-DCX (1:500, #ab113435, Abcam), rabbit polyclonal anti-OLIG2 (1:500, #AB9610, MilliporeSigma), rabbit polyclonal anti-NEUN (1:1000, #ABN78, MilliporeSigma), mouse monoclonal antiMASH1 (1:200, #556604, BD Biosciences), chicken polyclonal anti-RFP (1:500, #600-901-379, Rockland immunochemicals), rabbit polyclonal anti-H4K20ME3 (1:500, #pAb-057-050, Diagenode), rabbit polyclonal antiH3K9AC (1:500, #ab10812, Abcam).

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: Rabbit Polyclonal Anti-NeuN , Millipore , Cat# ABN78; RRID: AB_10807945.

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: For assessment of lesion pathology in the experimental animal model, the tissue sections of the thoracic spinal cord segments were incubated with diluted primary antibodies: rabbit polyclonal anti-NeuN (1:500; Millipore; Cat# ABN78; RRID: AB_10807945 ), mouse monoclonal anti-GFAP (1:500; Millipore; Cat# MAB360; RRID: AB_11212597 ), rabbit monoclonal anti-Vimentin (1:50; Cell Signaling Technology; Cat# 5741; RRID: AB_10695459 ), and rabbit polyclonal anti-GAP43 (1:500; Millipore; Cat# AB5220; RRID: AB_2107282 ).

    Positive Control:

    Article Title: SETD4 cells contribute to brain development and maintain adult stem cell reservoir for neurogenesis
    Article Snippet: The antibodies used in this study included mouse monoclonal anti-SETD4 (1:100, #sc-514060, Santa Cruz Biotechnology), rabbit polyclonal anti-GFAP (1:1000, #Z0334, Dako), goat polyclonal anti-GFAP (1:1000, #ab53554, Abcam), chicken polyclonal anti-NESTIN (1:1000, #NB100-1604, Novus Biologicals), mouse monoclonal anti-NESTIN (1:500, #MAB353, MilliporeSigma), rabbit polyclonal anti-SOX2 (1:200, #ab97959, Abcam), goat polyclonal anti-SOX2 (1:200, #AF2018, R&D Systems), rabbit monoclonal anti-KI67 (1:200, #ab16667, Abcam), mouse monoclonal antiPCNA (1:500, #ab29, Abcam), chicken polyclonal anti-GFP (1:1000, #ab13970, Abcam), rat monoclonal anti-BrdU (1:200, #ab6326, Abcam), goat polyclonal anti-DCX (1:500, #ab113435, Abcam), rabbit polyclonal anti-OLIG2 (1:500, #AB9610, MilliporeSigma), rabbit polyclonal anti-NEUN (1:1000, #ABN78, MilliporeSigma), mouse monoclonal antiMASH1 (1:200, #556604, BD Biosciences), chicken polyclonal anti-RFP (1:500, #600-901-379, Rockland immunochemicals), rabbit polyclonal anti-H4K20ME3 (1:500, #pAb-057-050, Diagenode), rabbit polyclonal antiH3K9AC (1:500, #ab10812, Abcam).

    Article Title: Interstitial-fluid shear stresses induced by vertically oscillating head motion lower blood pressure in hypertensive rats and humans.
    Article Snippet: Shuhei Murase, Naoyoshi Sakitani, Takahiro Maekawa, Daisuke Yoshino , Kouji Takano, Ayumu Konno , Hirokazu Hirai , Taku Saito , Sakae Tanaka, Keisuke Shinohara, Takuya Kishi, Yuki Yoshikawa , Takamasa Sakai , Makoto Ayaori, Hirohiko Inanami, Koji Tomiyasu, Atsushi Takashima, Toru Ogata , Hirotsugu Tsuchimochi , Shinya Sato, Shigeyoshi Saito, Kohzoh Yoshino, Yuiko Matsuura , Kenichi Funamoto , Hiroki Ochi, Masahiro Shinohara, Motoshi Nagao & Yasuhiro Sawada

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: Compound Sakura Finetek Cat# 4583 Vectashield Mounting Medium with DAPI Vector Laboratories Cat# H-1200-10 Critical commercial assays Jo urn al Pr e-p roo f Phosphorylated Neurofilament H Human ELISA BioVendor Cat# RD191138300R Corticosterone EIA IDS Cat# AC-14F1 BCA Protein Assay Kit Pierce Cat# 23225 Deposited data Raw data This paper https://doi.org/10.3494 5/F52P4M Experimental models: Organisms/strains Sprague-Dawley Rat Charles River Laboratories RRID: MGI: 5651135 Software and algorithms Kinema Tracer System Kissei Comtec http://www.kinematrac er.com/ Odyssey Application Software Version 3.0 LI-COR Biosciences RRID: SCR_014579 NIS Elements Imaging Software Nikon Instruments RRID: SCR_014329; https://calm.ucsf.edu/h ardware-and-softwareimage-analysis ImageJ NIH RRID: SCR_003070 AutoQuant Media Cybernetics RRID: SCR_002465 MetaMorph Automation and Image Analysis Software Molecular Devices RRID: SCR_002368 Adobe Photoshop Adobe RRID: SCR_014199 SPSS Statistics version 26.0 IBM RRID: SCR_016479 Graphpad Prism Graphpad RRID: SCR_002798 Adobe Illustrator Adobe RRID: SCR_010279 Other Infinite Horizon Impactor Precision Systems and Instrumentation IH-0400; https://psiimpactors.co m/product/ih400/ Treadmill Muromachi Kikai MK-680; https://muromachi.com /en/archives/english/19 36/ Biological Amplifier Nihon Kohden NEC Biotop 6R12; https://doi.org/10.1371/ journal.pone.0171937 AD Converter Cambridge Electronic Design Limited Micro 1401; https://ced.co.uk/produ cts/microwaveformio Constant Current Stimulator Digitimer DS7A; https://www.digitimer.c om/product/humanneurophysiology/periph eral-stimulators/ds7ads7ah-hv-currentstimulator/ Jo urn al P e-p roo f Plate Reader Tecan GENios; https://www.tecan.com/ customer-news/geniosan-innovativedetection-device-thatdelivers-flexibility-tothe-molecular-biologylaboratory-2347 Infrared Imaging System LI-COR Biosciences Odyssey Infrared Imaging System; RRID: SCR_023765; https://doi.org/10.1038/ s41598-024-70096-0 Cryostat Leica Biosystems CM1950; RRID: SCR_018061; https://www.leicabiosys tems.com/us/histologyequipment/cryostats/lei ca-cm1950/ Inverted Microscope Nikon Instruments ECLIPSE Ti inverted microscope system; RRID: SCR_021242; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Spinning Disk Confocal Microscope Yokogawa Electric Corporation CSU-22; RRID: SCR_020907; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Jo urn al Pr e-p roo f

    Article Title: Atp13a5 Marker Reveals Pericyte Specification in the Mouse Central Nervous System
    Article Snippet: Rabbit polyclonal anti-NeuN , Millipore Sigma , ABN78.

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: For assessment of lesion pathology in the experimental animal model, the tissue sections of the thoracic spinal cord segments were incubated with Jo urn al Pr e-p ro f 58 diluted primary antibodies: rabbit polyclonal anti-NeuN (1:500; Millipore; Cat# ABN78; RRID: AB_10807945), mouse monoclonal anti-GFAP (1:500; Millipore; Cat# MAB360; RRID: AB_11212597), rabbit monoclonal anti-Vimentin (1:50; Cell Signaling Technology; Cat# 5741; RRID: AB_10695459), and rabbit polyclonal anti-GAP43 (1:500; Millipore; Cat# AB5220; RRID: AB_2107282).

    Article Title: SETD4 cells contribute to brain development and maintain adult stem cell reservoir for neurogenesis
    Article Snippet: The antibodies used in this study included mouse monoclonal anti-SETD4 (1:100, #sc-514060, Santa Cruz Biotechnology), rabbit polyclonal anti-GFAP (1:1000, #Z0334, Dako), goat polyclonal anti-GFAP (1:1000, #ab53554, Abcam), chicken polyclonal anti-NESTIN (1:1000, #NB100-1604, Novus Biologicals), mouse monoclonal anti-NESTIN (1:500, #MAB353, MilliporeSigma), rabbit polyclonal anti-SOX2 (1:200, #ab97959, Abcam), goat polyclonal anti-SOX2 (1:200, #AF2018, R&D Systems), rabbit monoclonal anti-KI67 (1:200, #ab16667, Abcam), mouse monoclonal antiPCNA (1:500, #ab29, Abcam), chicken polyclonal anti-GFP (1:1000, #ab13970, Abcam), rat monoclonal anti-BrdU (1:200, #ab6326, Abcam), goat polyclonal anti-DCX (1:500, #ab113435, Abcam), rabbit polyclonal anti-OLIG2 (1:500, #AB9610, MilliporeSigma), rabbit polyclonal anti-NEUN (1:1000, #ABN78, MilliporeSigma), mouse monoclonal antiMASH1 (1:200, #556604, BD Biosciences), chicken polyclonal anti-RFP (1:500, #600-901-379, Rockland immunochemicals), rabbit polyclonal anti-H4K20ME3 (1:500, #pAb-057-050, Diagenode), rabbit polyclonal antiH3K9AC (1:500, #ab10812, Abcam).

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: Rabbit Polyclonal Anti-NeuN , Millipore , Cat# ABN78; RRID: AB_10807945.

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: For assessment of lesion pathology in the experimental animal model, the tissue sections of the thoracic spinal cord segments were incubated with diluted primary antibodies: rabbit polyclonal anti-NeuN (1:500; Millipore; Cat# ABN78; RRID: AB_10807945 ), mouse monoclonal anti-GFAP (1:500; Millipore; Cat# MAB360; RRID: AB_11212597 ), rabbit monoclonal anti-Vimentin (1:50; Cell Signaling Technology; Cat# 5741; RRID: AB_10695459 ), and rabbit polyclonal anti-GAP43 (1:500; Millipore; Cat# AB5220; RRID: AB_2107282 ).

    Negative Control:

    Article Title: SETD4 cells contribute to brain development and maintain adult stem cell reservoir for neurogenesis
    Article Snippet: The antibodies used in this study included mouse monoclonal anti-SETD4 (1:100, #sc-514060, Santa Cruz Biotechnology), rabbit polyclonal anti-GFAP (1:1000, #Z0334, Dako), goat polyclonal anti-GFAP (1:1000, #ab53554, Abcam), chicken polyclonal anti-NESTIN (1:1000, #NB100-1604, Novus Biologicals), mouse monoclonal anti-NESTIN (1:500, #MAB353, MilliporeSigma), rabbit polyclonal anti-SOX2 (1:200, #ab97959, Abcam), goat polyclonal anti-SOX2 (1:200, #AF2018, R&D Systems), rabbit monoclonal anti-KI67 (1:200, #ab16667, Abcam), mouse monoclonal antiPCNA (1:500, #ab29, Abcam), chicken polyclonal anti-GFP (1:1000, #ab13970, Abcam), rat monoclonal anti-BrdU (1:200, #ab6326, Abcam), goat polyclonal anti-DCX (1:500, #ab113435, Abcam), rabbit polyclonal anti-OLIG2 (1:500, #AB9610, MilliporeSigma), rabbit polyclonal anti-NEUN (1:1000, #ABN78, MilliporeSigma), mouse monoclonal antiMASH1 (1:200, #556604, BD Biosciences), chicken polyclonal anti-RFP (1:500, #600-901-379, Rockland immunochemicals), rabbit polyclonal anti-H4K20ME3 (1:500, #pAb-057-050, Diagenode), rabbit polyclonal antiH3K9AC (1:500, #ab10812, Abcam).

    Article Title: Interstitial-fluid shear stresses induced by vertically oscillating head motion lower blood pressure in hypertensive rats and humans.
    Article Snippet: Shuhei Murase, Naoyoshi Sakitani, Takahiro Maekawa, Daisuke Yoshino , Kouji Takano, Ayumu Konno , Hirokazu Hirai , Taku Saito , Sakae Tanaka, Keisuke Shinohara, Takuya Kishi, Yuki Yoshikawa , Takamasa Sakai , Makoto Ayaori, Hirohiko Inanami, Koji Tomiyasu, Atsushi Takashima, Toru Ogata , Hirotsugu Tsuchimochi , Shinya Sato, Shigeyoshi Saito, Kohzoh Yoshino, Yuiko Matsuura , Kenichi Funamoto , Hiroki Ochi, Masahiro Shinohara, Motoshi Nagao & Yasuhiro Sawada

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: Compound Sakura Finetek Cat# 4583 Vectashield Mounting Medium with DAPI Vector Laboratories Cat# H-1200-10 Critical commercial assays Jo urn al Pr e-p roo f Phosphorylated Neurofilament H Human ELISA BioVendor Cat# RD191138300R Corticosterone EIA IDS Cat# AC-14F1 BCA Protein Assay Kit Pierce Cat# 23225 Deposited data Raw data This paper https://doi.org/10.3494 5/F52P4M Experimental models: Organisms/strains Sprague-Dawley Rat Charles River Laboratories RRID: MGI: 5651135 Software and algorithms Kinema Tracer System Kissei Comtec http://www.kinematrac er.com/ Odyssey Application Software Version 3.0 LI-COR Biosciences RRID: SCR_014579 NIS Elements Imaging Software Nikon Instruments RRID: SCR_014329; https://calm.ucsf.edu/h ardware-and-softwareimage-analysis ImageJ NIH RRID: SCR_003070 AutoQuant Media Cybernetics RRID: SCR_002465 MetaMorph Automation and Image Analysis Software Molecular Devices RRID: SCR_002368 Adobe Photoshop Adobe RRID: SCR_014199 SPSS Statistics version 26.0 IBM RRID: SCR_016479 Graphpad Prism Graphpad RRID: SCR_002798 Adobe Illustrator Adobe RRID: SCR_010279 Other Infinite Horizon Impactor Precision Systems and Instrumentation IH-0400; https://psiimpactors.co m/product/ih400/ Treadmill Muromachi Kikai MK-680; https://muromachi.com /en/archives/english/19 36/ Biological Amplifier Nihon Kohden NEC Biotop 6R12; https://doi.org/10.1371/ journal.pone.0171937 AD Converter Cambridge Electronic Design Limited Micro 1401; https://ced.co.uk/produ cts/microwaveformio Constant Current Stimulator Digitimer DS7A; https://www.digitimer.c om/product/humanneurophysiology/periph eral-stimulators/ds7ads7ah-hv-currentstimulator/ Jo urn al P e-p roo f Plate Reader Tecan GENios; https://www.tecan.com/ customer-news/geniosan-innovativedetection-device-thatdelivers-flexibility-tothe-molecular-biologylaboratory-2347 Infrared Imaging System LI-COR Biosciences Odyssey Infrared Imaging System; RRID: SCR_023765; https://doi.org/10.1038/ s41598-024-70096-0 Cryostat Leica Biosystems CM1950; RRID: SCR_018061; https://www.leicabiosys tems.com/us/histologyequipment/cryostats/lei ca-cm1950/ Inverted Microscope Nikon Instruments ECLIPSE Ti inverted microscope system; RRID: SCR_021242; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Spinning Disk Confocal Microscope Yokogawa Electric Corporation CSU-22; RRID: SCR_020907; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Jo urn al Pr e-p roo f

    Article Title: Atp13a5 Marker Reveals Pericyte Specification in the Mouse Central Nervous System
    Article Snippet: Rabbit polyclonal anti-NeuN , Millipore Sigma , ABN78.

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: For assessment of lesion pathology in the experimental animal model, the tissue sections of the thoracic spinal cord segments were incubated with Jo urn al Pr e-p ro f 58 diluted primary antibodies: rabbit polyclonal anti-NeuN (1:500; Millipore; Cat# ABN78; RRID: AB_10807945), mouse monoclonal anti-GFAP (1:500; Millipore; Cat# MAB360; RRID: AB_11212597), rabbit monoclonal anti-Vimentin (1:50; Cell Signaling Technology; Cat# 5741; RRID: AB_10695459), and rabbit polyclonal anti-GAP43 (1:500; Millipore; Cat# AB5220; RRID: AB_2107282).

    Article Title: SETD4 cells contribute to brain development and maintain adult stem cell reservoir for neurogenesis
    Article Snippet: The antibodies used in this study included mouse monoclonal anti-SETD4 (1:100, #sc-514060, Santa Cruz Biotechnology), rabbit polyclonal anti-GFAP (1:1000, #Z0334, Dako), goat polyclonal anti-GFAP (1:1000, #ab53554, Abcam), chicken polyclonal anti-NESTIN (1:1000, #NB100-1604, Novus Biologicals), mouse monoclonal anti-NESTIN (1:500, #MAB353, MilliporeSigma), rabbit polyclonal anti-SOX2 (1:200, #ab97959, Abcam), goat polyclonal anti-SOX2 (1:200, #AF2018, R&D Systems), rabbit monoclonal anti-KI67 (1:200, #ab16667, Abcam), mouse monoclonal antiPCNA (1:500, #ab29, Abcam), chicken polyclonal anti-GFP (1:1000, #ab13970, Abcam), rat monoclonal anti-BrdU (1:200, #ab6326, Abcam), goat polyclonal anti-DCX (1:500, #ab113435, Abcam), rabbit polyclonal anti-OLIG2 (1:500, #AB9610, MilliporeSigma), rabbit polyclonal anti-NEUN (1:1000, #ABN78, MilliporeSigma), mouse monoclonal antiMASH1 (1:200, #556604, BD Biosciences), chicken polyclonal anti-RFP (1:500, #600-901-379, Rockland immunochemicals), rabbit polyclonal anti-H4K20ME3 (1:500, #pAb-057-050, Diagenode), rabbit polyclonal antiH3K9AC (1:500, #ab10812, Abcam).

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: Rabbit Polyclonal Anti-NeuN , Millipore , Cat# ABN78; RRID: AB_10807945.

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: For assessment of lesion pathology in the experimental animal model, the tissue sections of the thoracic spinal cord segments were incubated with diluted primary antibodies: rabbit polyclonal anti-NeuN (1:500; Millipore; Cat# ABN78; RRID: AB_10807945 ), mouse monoclonal anti-GFAP (1:500; Millipore; Cat# MAB360; RRID: AB_11212597 ), rabbit monoclonal anti-Vimentin (1:50; Cell Signaling Technology; Cat# 5741; RRID: AB_10695459 ), and rabbit polyclonal anti-GAP43 (1:500; Millipore; Cat# AB5220; RRID: AB_2107282 ).

    Multiplex Assay:

    Article Title: SETD4 cells contribute to brain development and maintain adult stem cell reservoir for neurogenesis
    Article Snippet: The antibodies used in this study included mouse monoclonal anti-SETD4 (1:100, #sc-514060, Santa Cruz Biotechnology), rabbit polyclonal anti-GFAP (1:1000, #Z0334, Dako), goat polyclonal anti-GFAP (1:1000, #ab53554, Abcam), chicken polyclonal anti-NESTIN (1:1000, #NB100-1604, Novus Biologicals), mouse monoclonal anti-NESTIN (1:500, #MAB353, MilliporeSigma), rabbit polyclonal anti-SOX2 (1:200, #ab97959, Abcam), goat polyclonal anti-SOX2 (1:200, #AF2018, R&D Systems), rabbit monoclonal anti-KI67 (1:200, #ab16667, Abcam), mouse monoclonal antiPCNA (1:500, #ab29, Abcam), chicken polyclonal anti-GFP (1:1000, #ab13970, Abcam), rat monoclonal anti-BrdU (1:200, #ab6326, Abcam), goat polyclonal anti-DCX (1:500, #ab113435, Abcam), rabbit polyclonal anti-OLIG2 (1:500, #AB9610, MilliporeSigma), rabbit polyclonal anti-NEUN (1:1000, #ABN78, MilliporeSigma), mouse monoclonal antiMASH1 (1:200, #556604, BD Biosciences), chicken polyclonal anti-RFP (1:500, #600-901-379, Rockland immunochemicals), rabbit polyclonal anti-H4K20ME3 (1:500, #pAb-057-050, Diagenode), rabbit polyclonal antiH3K9AC (1:500, #ab10812, Abcam).

    Article Title: Interstitial-fluid shear stresses induced by vertically oscillating head motion lower blood pressure in hypertensive rats and humans.
    Article Snippet: Shuhei Murase, Naoyoshi Sakitani, Takahiro Maekawa, Daisuke Yoshino , Kouji Takano, Ayumu Konno , Hirokazu Hirai , Taku Saito , Sakae Tanaka, Keisuke Shinohara, Takuya Kishi, Yuki Yoshikawa , Takamasa Sakai , Makoto Ayaori, Hirohiko Inanami, Koji Tomiyasu, Atsushi Takashima, Toru Ogata , Hirotsugu Tsuchimochi , Shinya Sato, Shigeyoshi Saito, Kohzoh Yoshino, Yuiko Matsuura , Kenichi Funamoto , Hiroki Ochi, Masahiro Shinohara, Motoshi Nagao & Yasuhiro Sawada

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: Compound Sakura Finetek Cat# 4583 Vectashield Mounting Medium with DAPI Vector Laboratories Cat# H-1200-10 Critical commercial assays Jo urn al Pr e-p roo f Phosphorylated Neurofilament H Human ELISA BioVendor Cat# RD191138300R Corticosterone EIA IDS Cat# AC-14F1 BCA Protein Assay Kit Pierce Cat# 23225 Deposited data Raw data This paper https://doi.org/10.3494 5/F52P4M Experimental models: Organisms/strains Sprague-Dawley Rat Charles River Laboratories RRID: MGI: 5651135 Software and algorithms Kinema Tracer System Kissei Comtec http://www.kinematrac er.com/ Odyssey Application Software Version 3.0 LI-COR Biosciences RRID: SCR_014579 NIS Elements Imaging Software Nikon Instruments RRID: SCR_014329; https://calm.ucsf.edu/h ardware-and-softwareimage-analysis ImageJ NIH RRID: SCR_003070 AutoQuant Media Cybernetics RRID: SCR_002465 MetaMorph Automation and Image Analysis Software Molecular Devices RRID: SCR_002368 Adobe Photoshop Adobe RRID: SCR_014199 SPSS Statistics version 26.0 IBM RRID: SCR_016479 Graphpad Prism Graphpad RRID: SCR_002798 Adobe Illustrator Adobe RRID: SCR_010279 Other Infinite Horizon Impactor Precision Systems and Instrumentation IH-0400; https://psiimpactors.co m/product/ih400/ Treadmill Muromachi Kikai MK-680; https://muromachi.com /en/archives/english/19 36/ Biological Amplifier Nihon Kohden NEC Biotop 6R12; https://doi.org/10.1371/ journal.pone.0171937 AD Converter Cambridge Electronic Design Limited Micro 1401; https://ced.co.uk/produ cts/microwaveformio Constant Current Stimulator Digitimer DS7A; https://www.digitimer.c om/product/humanneurophysiology/periph eral-stimulators/ds7ads7ah-hv-currentstimulator/ Jo urn al P e-p roo f Plate Reader Tecan GENios; https://www.tecan.com/ customer-news/geniosan-innovativedetection-device-thatdelivers-flexibility-tothe-molecular-biologylaboratory-2347 Infrared Imaging System LI-COR Biosciences Odyssey Infrared Imaging System; RRID: SCR_023765; https://doi.org/10.1038/ s41598-024-70096-0 Cryostat Leica Biosystems CM1950; RRID: SCR_018061; https://www.leicabiosys tems.com/us/histologyequipment/cryostats/lei ca-cm1950/ Inverted Microscope Nikon Instruments ECLIPSE Ti inverted microscope system; RRID: SCR_021242; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Spinning Disk Confocal Microscope Yokogawa Electric Corporation CSU-22; RRID: SCR_020907; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Jo urn al Pr e-p roo f

    Article Title: Atp13a5 Marker Reveals Pericyte Specification in the Mouse Central Nervous System
    Article Snippet: Rabbit polyclonal anti-NeuN , Millipore Sigma , ABN78.

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: For assessment of lesion pathology in the experimental animal model, the tissue sections of the thoracic spinal cord segments were incubated with Jo urn al Pr e-p ro f 58 diluted primary antibodies: rabbit polyclonal anti-NeuN (1:500; Millipore; Cat# ABN78; RRID: AB_10807945), mouse monoclonal anti-GFAP (1:500; Millipore; Cat# MAB360; RRID: AB_11212597), rabbit monoclonal anti-Vimentin (1:50; Cell Signaling Technology; Cat# 5741; RRID: AB_10695459), and rabbit polyclonal anti-GAP43 (1:500; Millipore; Cat# AB5220; RRID: AB_2107282).

    Article Title: SETD4 cells contribute to brain development and maintain adult stem cell reservoir for neurogenesis
    Article Snippet: The antibodies used in this study included mouse monoclonal anti-SETD4 (1:100, #sc-514060, Santa Cruz Biotechnology), rabbit polyclonal anti-GFAP (1:1000, #Z0334, Dako), goat polyclonal anti-GFAP (1:1000, #ab53554, Abcam), chicken polyclonal anti-NESTIN (1:1000, #NB100-1604, Novus Biologicals), mouse monoclonal anti-NESTIN (1:500, #MAB353, MilliporeSigma), rabbit polyclonal anti-SOX2 (1:200, #ab97959, Abcam), goat polyclonal anti-SOX2 (1:200, #AF2018, R&D Systems), rabbit monoclonal anti-KI67 (1:200, #ab16667, Abcam), mouse monoclonal antiPCNA (1:500, #ab29, Abcam), chicken polyclonal anti-GFP (1:1000, #ab13970, Abcam), rat monoclonal anti-BrdU (1:200, #ab6326, Abcam), goat polyclonal anti-DCX (1:500, #ab113435, Abcam), rabbit polyclonal anti-OLIG2 (1:500, #AB9610, MilliporeSigma), rabbit polyclonal anti-NEUN (1:1000, #ABN78, MilliporeSigma), mouse monoclonal antiMASH1 (1:200, #556604, BD Biosciences), chicken polyclonal anti-RFP (1:500, #600-901-379, Rockland immunochemicals), rabbit polyclonal anti-H4K20ME3 (1:500, #pAb-057-050, Diagenode), rabbit polyclonal antiH3K9AC (1:500, #ab10812, Abcam).

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: Rabbit Polyclonal Anti-NeuN , Millipore , Cat# ABN78; RRID: AB_10807945.

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: For assessment of lesion pathology in the experimental animal model, the tissue sections of the thoracic spinal cord segments were incubated with diluted primary antibodies: rabbit polyclonal anti-NeuN (1:500; Millipore; Cat# ABN78; RRID: AB_10807945 ), mouse monoclonal anti-GFAP (1:500; Millipore; Cat# MAB360; RRID: AB_11212597 ), rabbit monoclonal anti-Vimentin (1:50; Cell Signaling Technology; Cat# 5741; RRID: AB_10695459 ), and rabbit polyclonal anti-GAP43 (1:500; Millipore; Cat# AB5220; RRID: AB_2107282 ).

    Fluorescence:

    Article Title: SETD4 cells contribute to brain development and maintain adult stem cell reservoir for neurogenesis
    Article Snippet: The antibodies used in this study included mouse monoclonal anti-SETD4 (1:100, #sc-514060, Santa Cruz Biotechnology), rabbit polyclonal anti-GFAP (1:1000, #Z0334, Dako), goat polyclonal anti-GFAP (1:1000, #ab53554, Abcam), chicken polyclonal anti-NESTIN (1:1000, #NB100-1604, Novus Biologicals), mouse monoclonal anti-NESTIN (1:500, #MAB353, MilliporeSigma), rabbit polyclonal anti-SOX2 (1:200, #ab97959, Abcam), goat polyclonal anti-SOX2 (1:200, #AF2018, R&D Systems), rabbit monoclonal anti-KI67 (1:200, #ab16667, Abcam), mouse monoclonal antiPCNA (1:500, #ab29, Abcam), chicken polyclonal anti-GFP (1:1000, #ab13970, Abcam), rat monoclonal anti-BrdU (1:200, #ab6326, Abcam), goat polyclonal anti-DCX (1:500, #ab113435, Abcam), rabbit polyclonal anti-OLIG2 (1:500, #AB9610, MilliporeSigma), rabbit polyclonal anti-NEUN (1:1000, #ABN78, MilliporeSigma), mouse monoclonal antiMASH1 (1:200, #556604, BD Biosciences), chicken polyclonal anti-RFP (1:500, #600-901-379, Rockland immunochemicals), rabbit polyclonal anti-H4K20ME3 (1:500, #pAb-057-050, Diagenode), rabbit polyclonal antiH3K9AC (1:500, #ab10812, Abcam).

    Article Title: Interstitial-fluid shear stresses induced by vertically oscillating head motion lower blood pressure in hypertensive rats and humans.
    Article Snippet: Shuhei Murase, Naoyoshi Sakitani, Takahiro Maekawa, Daisuke Yoshino , Kouji Takano, Ayumu Konno , Hirokazu Hirai , Taku Saito , Sakae Tanaka, Keisuke Shinohara, Takuya Kishi, Yuki Yoshikawa , Takamasa Sakai , Makoto Ayaori, Hirohiko Inanami, Koji Tomiyasu, Atsushi Takashima, Toru Ogata , Hirotsugu Tsuchimochi , Shinya Sato, Shigeyoshi Saito, Kohzoh Yoshino, Yuiko Matsuura , Kenichi Funamoto , Hiroki Ochi, Masahiro Shinohara, Motoshi Nagao & Yasuhiro Sawada

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: Compound Sakura Finetek Cat# 4583 Vectashield Mounting Medium with DAPI Vector Laboratories Cat# H-1200-10 Critical commercial assays Jo urn al Pr e-p roo f Phosphorylated Neurofilament H Human ELISA BioVendor Cat# RD191138300R Corticosterone EIA IDS Cat# AC-14F1 BCA Protein Assay Kit Pierce Cat# 23225 Deposited data Raw data This paper https://doi.org/10.3494 5/F52P4M Experimental models: Organisms/strains Sprague-Dawley Rat Charles River Laboratories RRID: MGI: 5651135 Software and algorithms Kinema Tracer System Kissei Comtec http://www.kinematrac er.com/ Odyssey Application Software Version 3.0 LI-COR Biosciences RRID: SCR_014579 NIS Elements Imaging Software Nikon Instruments RRID: SCR_014329; https://calm.ucsf.edu/h ardware-and-softwareimage-analysis ImageJ NIH RRID: SCR_003070 AutoQuant Media Cybernetics RRID: SCR_002465 MetaMorph Automation and Image Analysis Software Molecular Devices RRID: SCR_002368 Adobe Photoshop Adobe RRID: SCR_014199 SPSS Statistics version 26.0 IBM RRID: SCR_016479 Graphpad Prism Graphpad RRID: SCR_002798 Adobe Illustrator Adobe RRID: SCR_010279 Other Infinite Horizon Impactor Precision Systems and Instrumentation IH-0400; https://psiimpactors.co m/product/ih400/ Treadmill Muromachi Kikai MK-680; https://muromachi.com /en/archives/english/19 36/ Biological Amplifier Nihon Kohden NEC Biotop 6R12; https://doi.org/10.1371/ journal.pone.0171937 AD Converter Cambridge Electronic Design Limited Micro 1401; https://ced.co.uk/produ cts/microwaveformio Constant Current Stimulator Digitimer DS7A; https://www.digitimer.c om/product/humanneurophysiology/periph eral-stimulators/ds7ads7ah-hv-currentstimulator/ Jo urn al P e-p roo f Plate Reader Tecan GENios; https://www.tecan.com/ customer-news/geniosan-innovativedetection-device-thatdelivers-flexibility-tothe-molecular-biologylaboratory-2347 Infrared Imaging System LI-COR Biosciences Odyssey Infrared Imaging System; RRID: SCR_023765; https://doi.org/10.1038/ s41598-024-70096-0 Cryostat Leica Biosystems CM1950; RRID: SCR_018061; https://www.leicabiosys tems.com/us/histologyequipment/cryostats/lei ca-cm1950/ Inverted Microscope Nikon Instruments ECLIPSE Ti inverted microscope system; RRID: SCR_021242; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Spinning Disk Confocal Microscope Yokogawa Electric Corporation CSU-22; RRID: SCR_020907; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Jo urn al Pr e-p roo f

    Article Title: Atp13a5 Marker Reveals Pericyte Specification in the Mouse Central Nervous System
    Article Snippet: Rabbit polyclonal anti-NeuN , Millipore Sigma , ABN78.

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: For assessment of lesion pathology in the experimental animal model, the tissue sections of the thoracic spinal cord segments were incubated with Jo urn al Pr e-p ro f 58 diluted primary antibodies: rabbit polyclonal anti-NeuN (1:500; Millipore; Cat# ABN78; RRID: AB_10807945), mouse monoclonal anti-GFAP (1:500; Millipore; Cat# MAB360; RRID: AB_11212597), rabbit monoclonal anti-Vimentin (1:50; Cell Signaling Technology; Cat# 5741; RRID: AB_10695459), and rabbit polyclonal anti-GAP43 (1:500; Millipore; Cat# AB5220; RRID: AB_2107282).

    Article Title: SETD4 cells contribute to brain development and maintain adult stem cell reservoir for neurogenesis
    Article Snippet: The antibodies used in this study included mouse monoclonal anti-SETD4 (1:100, #sc-514060, Santa Cruz Biotechnology), rabbit polyclonal anti-GFAP (1:1000, #Z0334, Dako), goat polyclonal anti-GFAP (1:1000, #ab53554, Abcam), chicken polyclonal anti-NESTIN (1:1000, #NB100-1604, Novus Biologicals), mouse monoclonal anti-NESTIN (1:500, #MAB353, MilliporeSigma), rabbit polyclonal anti-SOX2 (1:200, #ab97959, Abcam), goat polyclonal anti-SOX2 (1:200, #AF2018, R&D Systems), rabbit monoclonal anti-KI67 (1:200, #ab16667, Abcam), mouse monoclonal antiPCNA (1:500, #ab29, Abcam), chicken polyclonal anti-GFP (1:1000, #ab13970, Abcam), rat monoclonal anti-BrdU (1:200, #ab6326, Abcam), goat polyclonal anti-DCX (1:500, #ab113435, Abcam), rabbit polyclonal anti-OLIG2 (1:500, #AB9610, MilliporeSigma), rabbit polyclonal anti-NEUN (1:1000, #ABN78, MilliporeSigma), mouse monoclonal antiMASH1 (1:200, #556604, BD Biosciences), chicken polyclonal anti-RFP (1:500, #600-901-379, Rockland immunochemicals), rabbit polyclonal anti-H4K20ME3 (1:500, #pAb-057-050, Diagenode), rabbit polyclonal antiH3K9AC (1:500, #ab10812, Abcam).

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: Rabbit Polyclonal Anti-NeuN , Millipore , Cat# ABN78; RRID: AB_10807945.

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: For assessment of lesion pathology in the experimental animal model, the tissue sections of the thoracic spinal cord segments were incubated with diluted primary antibodies: rabbit polyclonal anti-NeuN (1:500; Millipore; Cat# ABN78; RRID: AB_10807945 ), mouse monoclonal anti-GFAP (1:500; Millipore; Cat# MAB360; RRID: AB_11212597 ), rabbit monoclonal anti-Vimentin (1:50; Cell Signaling Technology; Cat# 5741; RRID: AB_10695459 ), and rabbit polyclonal anti-GAP43 (1:500; Millipore; Cat# AB5220; RRID: AB_2107282 ).

    Chromatin Immunoprecipitation:

    Article Title: SETD4 cells contribute to brain development and maintain adult stem cell reservoir for neurogenesis
    Article Snippet: The antibodies used in this study included mouse monoclonal anti-SETD4 (1:100, #sc-514060, Santa Cruz Biotechnology), rabbit polyclonal anti-GFAP (1:1000, #Z0334, Dako), goat polyclonal anti-GFAP (1:1000, #ab53554, Abcam), chicken polyclonal anti-NESTIN (1:1000, #NB100-1604, Novus Biologicals), mouse monoclonal anti-NESTIN (1:500, #MAB353, MilliporeSigma), rabbit polyclonal anti-SOX2 (1:200, #ab97959, Abcam), goat polyclonal anti-SOX2 (1:200, #AF2018, R&D Systems), rabbit monoclonal anti-KI67 (1:200, #ab16667, Abcam), mouse monoclonal antiPCNA (1:500, #ab29, Abcam), chicken polyclonal anti-GFP (1:1000, #ab13970, Abcam), rat monoclonal anti-BrdU (1:200, #ab6326, Abcam), goat polyclonal anti-DCX (1:500, #ab113435, Abcam), rabbit polyclonal anti-OLIG2 (1:500, #AB9610, MilliporeSigma), rabbit polyclonal anti-NEUN (1:1000, #ABN78, MilliporeSigma), mouse monoclonal antiMASH1 (1:200, #556604, BD Biosciences), chicken polyclonal anti-RFP (1:500, #600-901-379, Rockland immunochemicals), rabbit polyclonal anti-H4K20ME3 (1:500, #pAb-057-050, Diagenode), rabbit polyclonal antiH3K9AC (1:500, #ab10812, Abcam).

    Article Title: Interstitial-fluid shear stresses induced by vertically oscillating head motion lower blood pressure in hypertensive rats and humans.
    Article Snippet: Shuhei Murase, Naoyoshi Sakitani, Takahiro Maekawa, Daisuke Yoshino , Kouji Takano, Ayumu Konno , Hirokazu Hirai , Taku Saito , Sakae Tanaka, Keisuke Shinohara, Takuya Kishi, Yuki Yoshikawa , Takamasa Sakai , Makoto Ayaori, Hirohiko Inanami, Koji Tomiyasu, Atsushi Takashima, Toru Ogata , Hirotsugu Tsuchimochi , Shinya Sato, Shigeyoshi Saito, Kohzoh Yoshino, Yuiko Matsuura , Kenichi Funamoto , Hiroki Ochi, Masahiro Shinohara, Motoshi Nagao & Yasuhiro Sawada

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: Compound Sakura Finetek Cat# 4583 Vectashield Mounting Medium with DAPI Vector Laboratories Cat# H-1200-10 Critical commercial assays Jo urn al Pr e-p roo f Phosphorylated Neurofilament H Human ELISA BioVendor Cat# RD191138300R Corticosterone EIA IDS Cat# AC-14F1 BCA Protein Assay Kit Pierce Cat# 23225 Deposited data Raw data This paper https://doi.org/10.3494 5/F52P4M Experimental models: Organisms/strains Sprague-Dawley Rat Charles River Laboratories RRID: MGI: 5651135 Software and algorithms Kinema Tracer System Kissei Comtec http://www.kinematrac er.com/ Odyssey Application Software Version 3.0 LI-COR Biosciences RRID: SCR_014579 NIS Elements Imaging Software Nikon Instruments RRID: SCR_014329; https://calm.ucsf.edu/h ardware-and-softwareimage-analysis ImageJ NIH RRID: SCR_003070 AutoQuant Media Cybernetics RRID: SCR_002465 MetaMorph Automation and Image Analysis Software Molecular Devices RRID: SCR_002368 Adobe Photoshop Adobe RRID: SCR_014199 SPSS Statistics version 26.0 IBM RRID: SCR_016479 Graphpad Prism Graphpad RRID: SCR_002798 Adobe Illustrator Adobe RRID: SCR_010279 Other Infinite Horizon Impactor Precision Systems and Instrumentation IH-0400; https://psiimpactors.co m/product/ih400/ Treadmill Muromachi Kikai MK-680; https://muromachi.com /en/archives/english/19 36/ Biological Amplifier Nihon Kohden NEC Biotop 6R12; https://doi.org/10.1371/ journal.pone.0171937 AD Converter Cambridge Electronic Design Limited Micro 1401; https://ced.co.uk/produ cts/microwaveformio Constant Current Stimulator Digitimer DS7A; https://www.digitimer.c om/product/humanneurophysiology/periph eral-stimulators/ds7ads7ah-hv-currentstimulator/ Jo urn al P e-p roo f Plate Reader Tecan GENios; https://www.tecan.com/ customer-news/geniosan-innovativedetection-device-thatdelivers-flexibility-tothe-molecular-biologylaboratory-2347 Infrared Imaging System LI-COR Biosciences Odyssey Infrared Imaging System; RRID: SCR_023765; https://doi.org/10.1038/ s41598-024-70096-0 Cryostat Leica Biosystems CM1950; RRID: SCR_018061; https://www.leicabiosys tems.com/us/histologyequipment/cryostats/lei ca-cm1950/ Inverted Microscope Nikon Instruments ECLIPSE Ti inverted microscope system; RRID: SCR_021242; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Spinning Disk Confocal Microscope Yokogawa Electric Corporation CSU-22; RRID: SCR_020907; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Jo urn al Pr e-p roo f

    Article Title: Atp13a5 Marker Reveals Pericyte Specification in the Mouse Central Nervous System
    Article Snippet: Rabbit polyclonal anti-NeuN , Millipore Sigma , ABN78.

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: For assessment of lesion pathology in the experimental animal model, the tissue sections of the thoracic spinal cord segments were incubated with Jo urn al Pr e-p ro f 58 diluted primary antibodies: rabbit polyclonal anti-NeuN (1:500; Millipore; Cat# ABN78; RRID: AB_10807945), mouse monoclonal anti-GFAP (1:500; Millipore; Cat# MAB360; RRID: AB_11212597), rabbit monoclonal anti-Vimentin (1:50; Cell Signaling Technology; Cat# 5741; RRID: AB_10695459), and rabbit polyclonal anti-GAP43 (1:500; Millipore; Cat# AB5220; RRID: AB_2107282).

    Article Title: SETD4 cells contribute to brain development and maintain adult stem cell reservoir for neurogenesis
    Article Snippet: The antibodies used in this study included mouse monoclonal anti-SETD4 (1:100, #sc-514060, Santa Cruz Biotechnology), rabbit polyclonal anti-GFAP (1:1000, #Z0334, Dako), goat polyclonal anti-GFAP (1:1000, #ab53554, Abcam), chicken polyclonal anti-NESTIN (1:1000, #NB100-1604, Novus Biologicals), mouse monoclonal anti-NESTIN (1:500, #MAB353, MilliporeSigma), rabbit polyclonal anti-SOX2 (1:200, #ab97959, Abcam), goat polyclonal anti-SOX2 (1:200, #AF2018, R&D Systems), rabbit monoclonal anti-KI67 (1:200, #ab16667, Abcam), mouse monoclonal antiPCNA (1:500, #ab29, Abcam), chicken polyclonal anti-GFP (1:1000, #ab13970, Abcam), rat monoclonal anti-BrdU (1:200, #ab6326, Abcam), goat polyclonal anti-DCX (1:500, #ab113435, Abcam), rabbit polyclonal anti-OLIG2 (1:500, #AB9610, MilliporeSigma), rabbit polyclonal anti-NEUN (1:1000, #ABN78, MilliporeSigma), mouse monoclonal antiMASH1 (1:200, #556604, BD Biosciences), chicken polyclonal anti-RFP (1:500, #600-901-379, Rockland immunochemicals), rabbit polyclonal anti-H4K20ME3 (1:500, #pAb-057-050, Diagenode), rabbit polyclonal antiH3K9AC (1:500, #ab10812, Abcam).

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: Rabbit Polyclonal Anti-NeuN , Millipore , Cat# ABN78; RRID: AB_10807945.

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: For assessment of lesion pathology in the experimental animal model, the tissue sections of the thoracic spinal cord segments were incubated with diluted primary antibodies: rabbit polyclonal anti-NeuN (1:500; Millipore; Cat# ABN78; RRID: AB_10807945 ), mouse monoclonal anti-GFAP (1:500; Millipore; Cat# MAB360; RRID: AB_11212597 ), rabbit monoclonal anti-Vimentin (1:50; Cell Signaling Technology; Cat# 5741; RRID: AB_10695459 ), and rabbit polyclonal anti-GAP43 (1:500; Millipore; Cat# AB5220; RRID: AB_2107282 ).

    Flow Cytometry:

    Article Title: SETD4 cells contribute to brain development and maintain adult stem cell reservoir for neurogenesis
    Article Snippet: The antibodies used in this study included mouse monoclonal anti-SETD4 (1:100, #sc-514060, Santa Cruz Biotechnology), rabbit polyclonal anti-GFAP (1:1000, #Z0334, Dako), goat polyclonal anti-GFAP (1:1000, #ab53554, Abcam), chicken polyclonal anti-NESTIN (1:1000, #NB100-1604, Novus Biologicals), mouse monoclonal anti-NESTIN (1:500, #MAB353, MilliporeSigma), rabbit polyclonal anti-SOX2 (1:200, #ab97959, Abcam), goat polyclonal anti-SOX2 (1:200, #AF2018, R&D Systems), rabbit monoclonal anti-KI67 (1:200, #ab16667, Abcam), mouse monoclonal antiPCNA (1:500, #ab29, Abcam), chicken polyclonal anti-GFP (1:1000, #ab13970, Abcam), rat monoclonal anti-BrdU (1:200, #ab6326, Abcam), goat polyclonal anti-DCX (1:500, #ab113435, Abcam), rabbit polyclonal anti-OLIG2 (1:500, #AB9610, MilliporeSigma), rabbit polyclonal anti-NEUN (1:1000, #ABN78, MilliporeSigma), mouse monoclonal antiMASH1 (1:200, #556604, BD Biosciences), chicken polyclonal anti-RFP (1:500, #600-901-379, Rockland immunochemicals), rabbit polyclonal anti-H4K20ME3 (1:500, #pAb-057-050, Diagenode), rabbit polyclonal antiH3K9AC (1:500, #ab10812, Abcam).

    Article Title: Interstitial-fluid shear stresses induced by vertically oscillating head motion lower blood pressure in hypertensive rats and humans.
    Article Snippet: Shuhei Murase, Naoyoshi Sakitani, Takahiro Maekawa, Daisuke Yoshino , Kouji Takano, Ayumu Konno , Hirokazu Hirai , Taku Saito , Sakae Tanaka, Keisuke Shinohara, Takuya Kishi, Yuki Yoshikawa , Takamasa Sakai , Makoto Ayaori, Hirohiko Inanami, Koji Tomiyasu, Atsushi Takashima, Toru Ogata , Hirotsugu Tsuchimochi , Shinya Sato, Shigeyoshi Saito, Kohzoh Yoshino, Yuiko Matsuura , Kenichi Funamoto , Hiroki Ochi, Masahiro Shinohara, Motoshi Nagao & Yasuhiro Sawada

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: Compound Sakura Finetek Cat# 4583 Vectashield Mounting Medium with DAPI Vector Laboratories Cat# H-1200-10 Critical commercial assays Jo urn al Pr e-p roo f Phosphorylated Neurofilament H Human ELISA BioVendor Cat# RD191138300R Corticosterone EIA IDS Cat# AC-14F1 BCA Protein Assay Kit Pierce Cat# 23225 Deposited data Raw data This paper https://doi.org/10.3494 5/F52P4M Experimental models: Organisms/strains Sprague-Dawley Rat Charles River Laboratories RRID: MGI: 5651135 Software and algorithms Kinema Tracer System Kissei Comtec http://www.kinematrac er.com/ Odyssey Application Software Version 3.0 LI-COR Biosciences RRID: SCR_014579 NIS Elements Imaging Software Nikon Instruments RRID: SCR_014329; https://calm.ucsf.edu/h ardware-and-softwareimage-analysis ImageJ NIH RRID: SCR_003070 AutoQuant Media Cybernetics RRID: SCR_002465 MetaMorph Automation and Image Analysis Software Molecular Devices RRID: SCR_002368 Adobe Photoshop Adobe RRID: SCR_014199 SPSS Statistics version 26.0 IBM RRID: SCR_016479 Graphpad Prism Graphpad RRID: SCR_002798 Adobe Illustrator Adobe RRID: SCR_010279 Other Infinite Horizon Impactor Precision Systems and Instrumentation IH-0400; https://psiimpactors.co m/product/ih400/ Treadmill Muromachi Kikai MK-680; https://muromachi.com /en/archives/english/19 36/ Biological Amplifier Nihon Kohden NEC Biotop 6R12; https://doi.org/10.1371/ journal.pone.0171937 AD Converter Cambridge Electronic Design Limited Micro 1401; https://ced.co.uk/produ cts/microwaveformio Constant Current Stimulator Digitimer DS7A; https://www.digitimer.c om/product/humanneurophysiology/periph eral-stimulators/ds7ads7ah-hv-currentstimulator/ Jo urn al P e-p roo f Plate Reader Tecan GENios; https://www.tecan.com/ customer-news/geniosan-innovativedetection-device-thatdelivers-flexibility-tothe-molecular-biologylaboratory-2347 Infrared Imaging System LI-COR Biosciences Odyssey Infrared Imaging System; RRID: SCR_023765; https://doi.org/10.1038/ s41598-024-70096-0 Cryostat Leica Biosystems CM1950; RRID: SCR_018061; https://www.leicabiosys tems.com/us/histologyequipment/cryostats/lei ca-cm1950/ Inverted Microscope Nikon Instruments ECLIPSE Ti inverted microscope system; RRID: SCR_021242; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Spinning Disk Confocal Microscope Yokogawa Electric Corporation CSU-22; RRID: SCR_020907; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Jo urn al Pr e-p roo f

    Article Title: Atp13a5 Marker Reveals Pericyte Specification in the Mouse Central Nervous System
    Article Snippet: Rabbit polyclonal anti-NeuN , Millipore Sigma , ABN78.

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: For assessment of lesion pathology in the experimental animal model, the tissue sections of the thoracic spinal cord segments were incubated with Jo urn al Pr e-p ro f 58 diluted primary antibodies: rabbit polyclonal anti-NeuN (1:500; Millipore; Cat# ABN78; RRID: AB_10807945), mouse monoclonal anti-GFAP (1:500; Millipore; Cat# MAB360; RRID: AB_11212597), rabbit monoclonal anti-Vimentin (1:50; Cell Signaling Technology; Cat# 5741; RRID: AB_10695459), and rabbit polyclonal anti-GAP43 (1:500; Millipore; Cat# AB5220; RRID: AB_2107282).

    Article Title: SETD4 cells contribute to brain development and maintain adult stem cell reservoir for neurogenesis
    Article Snippet: The antibodies used in this study included mouse monoclonal anti-SETD4 (1:100, #sc-514060, Santa Cruz Biotechnology), rabbit polyclonal anti-GFAP (1:1000, #Z0334, Dako), goat polyclonal anti-GFAP (1:1000, #ab53554, Abcam), chicken polyclonal anti-NESTIN (1:1000, #NB100-1604, Novus Biologicals), mouse monoclonal anti-NESTIN (1:500, #MAB353, MilliporeSigma), rabbit polyclonal anti-SOX2 (1:200, #ab97959, Abcam), goat polyclonal anti-SOX2 (1:200, #AF2018, R&D Systems), rabbit monoclonal anti-KI67 (1:200, #ab16667, Abcam), mouse monoclonal antiPCNA (1:500, #ab29, Abcam), chicken polyclonal anti-GFP (1:1000, #ab13970, Abcam), rat monoclonal anti-BrdU (1:200, #ab6326, Abcam), goat polyclonal anti-DCX (1:500, #ab113435, Abcam), rabbit polyclonal anti-OLIG2 (1:500, #AB9610, MilliporeSigma), rabbit polyclonal anti-NEUN (1:1000, #ABN78, MilliporeSigma), mouse monoclonal antiMASH1 (1:200, #556604, BD Biosciences), chicken polyclonal anti-RFP (1:500, #600-901-379, Rockland immunochemicals), rabbit polyclonal anti-H4K20ME3 (1:500, #pAb-057-050, Diagenode), rabbit polyclonal antiH3K9AC (1:500, #ab10812, Abcam).

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: Rabbit Polyclonal Anti-NeuN , Millipore , Cat# ABN78; RRID: AB_10807945.

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: For assessment of lesion pathology in the experimental animal model, the tissue sections of the thoracic spinal cord segments were incubated with diluted primary antibodies: rabbit polyclonal anti-NeuN (1:500; Millipore; Cat# ABN78; RRID: AB_10807945 ), mouse monoclonal anti-GFAP (1:500; Millipore; Cat# MAB360; RRID: AB_11212597 ), rabbit monoclonal anti-Vimentin (1:50; Cell Signaling Technology; Cat# 5741; RRID: AB_10695459 ), and rabbit polyclonal anti-GAP43 (1:500; Millipore; Cat# AB5220; RRID: AB_2107282 ).

    Magnetic Resonance Imaging:

    Article Title: SETD4 cells contribute to brain development and maintain adult stem cell reservoir for neurogenesis
    Article Snippet: The antibodies used in this study included mouse monoclonal anti-SETD4 (1:100, #sc-514060, Santa Cruz Biotechnology), rabbit polyclonal anti-GFAP (1:1000, #Z0334, Dako), goat polyclonal anti-GFAP (1:1000, #ab53554, Abcam), chicken polyclonal anti-NESTIN (1:1000, #NB100-1604, Novus Biologicals), mouse monoclonal anti-NESTIN (1:500, #MAB353, MilliporeSigma), rabbit polyclonal anti-SOX2 (1:200, #ab97959, Abcam), goat polyclonal anti-SOX2 (1:200, #AF2018, R&D Systems), rabbit monoclonal anti-KI67 (1:200, #ab16667, Abcam), mouse monoclonal antiPCNA (1:500, #ab29, Abcam), chicken polyclonal anti-GFP (1:1000, #ab13970, Abcam), rat monoclonal anti-BrdU (1:200, #ab6326, Abcam), goat polyclonal anti-DCX (1:500, #ab113435, Abcam), rabbit polyclonal anti-OLIG2 (1:500, #AB9610, MilliporeSigma), rabbit polyclonal anti-NEUN (1:1000, #ABN78, MilliporeSigma), mouse monoclonal antiMASH1 (1:200, #556604, BD Biosciences), chicken polyclonal anti-RFP (1:500, #600-901-379, Rockland immunochemicals), rabbit polyclonal anti-H4K20ME3 (1:500, #pAb-057-050, Diagenode), rabbit polyclonal antiH3K9AC (1:500, #ab10812, Abcam).

    Article Title: Interstitial-fluid shear stresses induced by vertically oscillating head motion lower blood pressure in hypertensive rats and humans.
    Article Snippet: Shuhei Murase, Naoyoshi Sakitani, Takahiro Maekawa, Daisuke Yoshino , Kouji Takano, Ayumu Konno , Hirokazu Hirai , Taku Saito , Sakae Tanaka, Keisuke Shinohara, Takuya Kishi, Yuki Yoshikawa , Takamasa Sakai , Makoto Ayaori, Hirohiko Inanami, Koji Tomiyasu, Atsushi Takashima, Toru Ogata , Hirotsugu Tsuchimochi , Shinya Sato, Shigeyoshi Saito, Kohzoh Yoshino, Yuiko Matsuura , Kenichi Funamoto , Hiroki Ochi, Masahiro Shinohara, Motoshi Nagao & Yasuhiro Sawada

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: Compound Sakura Finetek Cat# 4583 Vectashield Mounting Medium with DAPI Vector Laboratories Cat# H-1200-10 Critical commercial assays Jo urn al Pr e-p roo f Phosphorylated Neurofilament H Human ELISA BioVendor Cat# RD191138300R Corticosterone EIA IDS Cat# AC-14F1 BCA Protein Assay Kit Pierce Cat# 23225 Deposited data Raw data This paper https://doi.org/10.3494 5/F52P4M Experimental models: Organisms/strains Sprague-Dawley Rat Charles River Laboratories RRID: MGI: 5651135 Software and algorithms Kinema Tracer System Kissei Comtec http://www.kinematrac er.com/ Odyssey Application Software Version 3.0 LI-COR Biosciences RRID: SCR_014579 NIS Elements Imaging Software Nikon Instruments RRID: SCR_014329; https://calm.ucsf.edu/h ardware-and-softwareimage-analysis ImageJ NIH RRID: SCR_003070 AutoQuant Media Cybernetics RRID: SCR_002465 MetaMorph Automation and Image Analysis Software Molecular Devices RRID: SCR_002368 Adobe Photoshop Adobe RRID: SCR_014199 SPSS Statistics version 26.0 IBM RRID: SCR_016479 Graphpad Prism Graphpad RRID: SCR_002798 Adobe Illustrator Adobe RRID: SCR_010279 Other Infinite Horizon Impactor Precision Systems and Instrumentation IH-0400; https://psiimpactors.co m/product/ih400/ Treadmill Muromachi Kikai MK-680; https://muromachi.com /en/archives/english/19 36/ Biological Amplifier Nihon Kohden NEC Biotop 6R12; https://doi.org/10.1371/ journal.pone.0171937 AD Converter Cambridge Electronic Design Limited Micro 1401; https://ced.co.uk/produ cts/microwaveformio Constant Current Stimulator Digitimer DS7A; https://www.digitimer.c om/product/humanneurophysiology/periph eral-stimulators/ds7ads7ah-hv-currentstimulator/ Jo urn al P e-p roo f Plate Reader Tecan GENios; https://www.tecan.com/ customer-news/geniosan-innovativedetection-device-thatdelivers-flexibility-tothe-molecular-biologylaboratory-2347 Infrared Imaging System LI-COR Biosciences Odyssey Infrared Imaging System; RRID: SCR_023765; https://doi.org/10.1038/ s41598-024-70096-0 Cryostat Leica Biosystems CM1950; RRID: SCR_018061; https://www.leicabiosys tems.com/us/histologyequipment/cryostats/lei ca-cm1950/ Inverted Microscope Nikon Instruments ECLIPSE Ti inverted microscope system; RRID: SCR_021242; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Spinning Disk Confocal Microscope Yokogawa Electric Corporation CSU-22; RRID: SCR_020907; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Jo urn al Pr e-p roo f

    Article Title: Atp13a5 Marker Reveals Pericyte Specification in the Mouse Central Nervous System
    Article Snippet: Rabbit polyclonal anti-NeuN , Millipore Sigma , ABN78.

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: For assessment of lesion pathology in the experimental animal model, the tissue sections of the thoracic spinal cord segments were incubated with Jo urn al Pr e-p ro f 58 diluted primary antibodies: rabbit polyclonal anti-NeuN (1:500; Millipore; Cat# ABN78; RRID: AB_10807945), mouse monoclonal anti-GFAP (1:500; Millipore; Cat# MAB360; RRID: AB_11212597), rabbit monoclonal anti-Vimentin (1:50; Cell Signaling Technology; Cat# 5741; RRID: AB_10695459), and rabbit polyclonal anti-GAP43 (1:500; Millipore; Cat# AB5220; RRID: AB_2107282).

    Article Title: SETD4 cells contribute to brain development and maintain adult stem cell reservoir for neurogenesis
    Article Snippet: The antibodies used in this study included mouse monoclonal anti-SETD4 (1:100, #sc-514060, Santa Cruz Biotechnology), rabbit polyclonal anti-GFAP (1:1000, #Z0334, Dako), goat polyclonal anti-GFAP (1:1000, #ab53554, Abcam), chicken polyclonal anti-NESTIN (1:1000, #NB100-1604, Novus Biologicals), mouse monoclonal anti-NESTIN (1:500, #MAB353, MilliporeSigma), rabbit polyclonal anti-SOX2 (1:200, #ab97959, Abcam), goat polyclonal anti-SOX2 (1:200, #AF2018, R&D Systems), rabbit monoclonal anti-KI67 (1:200, #ab16667, Abcam), mouse monoclonal antiPCNA (1:500, #ab29, Abcam), chicken polyclonal anti-GFP (1:1000, #ab13970, Abcam), rat monoclonal anti-BrdU (1:200, #ab6326, Abcam), goat polyclonal anti-DCX (1:500, #ab113435, Abcam), rabbit polyclonal anti-OLIG2 (1:500, #AB9610, MilliporeSigma), rabbit polyclonal anti-NEUN (1:1000, #ABN78, MilliporeSigma), mouse monoclonal antiMASH1 (1:200, #556604, BD Biosciences), chicken polyclonal anti-RFP (1:500, #600-901-379, Rockland immunochemicals), rabbit polyclonal anti-H4K20ME3 (1:500, #pAb-057-050, Diagenode), rabbit polyclonal antiH3K9AC (1:500, #ab10812, Abcam).

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: Rabbit Polyclonal Anti-NeuN , Millipore , Cat# ABN78; RRID: AB_10807945.

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: For assessment of lesion pathology in the experimental animal model, the tissue sections of the thoracic spinal cord segments were incubated with diluted primary antibodies: rabbit polyclonal anti-NeuN (1:500; Millipore; Cat# ABN78; RRID: AB_10807945 ), mouse monoclonal anti-GFAP (1:500; Millipore; Cat# MAB360; RRID: AB_11212597 ), rabbit monoclonal anti-Vimentin (1:50; Cell Signaling Technology; Cat# 5741; RRID: AB_10695459 ), and rabbit polyclonal anti-GAP43 (1:500; Millipore; Cat# AB5220; RRID: AB_2107282 ).

    Immunostaining:

    Article Title: SETD4 cells contribute to brain development and maintain adult stem cell reservoir for neurogenesis
    Article Snippet: The antibodies used in this study included mouse monoclonal anti-SETD4 (1:100, #sc-514060, Santa Cruz Biotechnology), rabbit polyclonal anti-GFAP (1:1000, #Z0334, Dako), goat polyclonal anti-GFAP (1:1000, #ab53554, Abcam), chicken polyclonal anti-NESTIN (1:1000, #NB100-1604, Novus Biologicals), mouse monoclonal anti-NESTIN (1:500, #MAB353, MilliporeSigma), rabbit polyclonal anti-SOX2 (1:200, #ab97959, Abcam), goat polyclonal anti-SOX2 (1:200, #AF2018, R&D Systems), rabbit monoclonal anti-KI67 (1:200, #ab16667, Abcam), mouse monoclonal antiPCNA (1:500, #ab29, Abcam), chicken polyclonal anti-GFP (1:1000, #ab13970, Abcam), rat monoclonal anti-BrdU (1:200, #ab6326, Abcam), goat polyclonal anti-DCX (1:500, #ab113435, Abcam), rabbit polyclonal anti-OLIG2 (1:500, #AB9610, MilliporeSigma), rabbit polyclonal anti-NEUN (1:1000, #ABN78, MilliporeSigma), mouse monoclonal antiMASH1 (1:200, #556604, BD Biosciences), chicken polyclonal anti-RFP (1:500, #600-901-379, Rockland immunochemicals), rabbit polyclonal anti-H4K20ME3 (1:500, #pAb-057-050, Diagenode), rabbit polyclonal antiH3K9AC (1:500, #ab10812, Abcam).

    Article Title: Interstitial-fluid shear stresses induced by vertically oscillating head motion lower blood pressure in hypertensive rats and humans.
    Article Snippet: Shuhei Murase, Naoyoshi Sakitani, Takahiro Maekawa, Daisuke Yoshino , Kouji Takano, Ayumu Konno , Hirokazu Hirai , Taku Saito , Sakae Tanaka, Keisuke Shinohara, Takuya Kishi, Yuki Yoshikawa , Takamasa Sakai , Makoto Ayaori, Hirohiko Inanami, Koji Tomiyasu, Atsushi Takashima, Toru Ogata , Hirotsugu Tsuchimochi , Shinya Sato, Shigeyoshi Saito, Kohzoh Yoshino, Yuiko Matsuura , Kenichi Funamoto , Hiroki Ochi, Masahiro Shinohara, Motoshi Nagao & Yasuhiro Sawada

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: Compound Sakura Finetek Cat# 4583 Vectashield Mounting Medium with DAPI Vector Laboratories Cat# H-1200-10 Critical commercial assays Jo urn al Pr e-p roo f Phosphorylated Neurofilament H Human ELISA BioVendor Cat# RD191138300R Corticosterone EIA IDS Cat# AC-14F1 BCA Protein Assay Kit Pierce Cat# 23225 Deposited data Raw data This paper https://doi.org/10.3494 5/F52P4M Experimental models: Organisms/strains Sprague-Dawley Rat Charles River Laboratories RRID: MGI: 5651135 Software and algorithms Kinema Tracer System Kissei Comtec http://www.kinematrac er.com/ Odyssey Application Software Version 3.0 LI-COR Biosciences RRID: SCR_014579 NIS Elements Imaging Software Nikon Instruments RRID: SCR_014329; https://calm.ucsf.edu/h ardware-and-softwareimage-analysis ImageJ NIH RRID: SCR_003070 AutoQuant Media Cybernetics RRID: SCR_002465 MetaMorph Automation and Image Analysis Software Molecular Devices RRID: SCR_002368 Adobe Photoshop Adobe RRID: SCR_014199 SPSS Statistics version 26.0 IBM RRID: SCR_016479 Graphpad Prism Graphpad RRID: SCR_002798 Adobe Illustrator Adobe RRID: SCR_010279 Other Infinite Horizon Impactor Precision Systems and Instrumentation IH-0400; https://psiimpactors.co m/product/ih400/ Treadmill Muromachi Kikai MK-680; https://muromachi.com /en/archives/english/19 36/ Biological Amplifier Nihon Kohden NEC Biotop 6R12; https://doi.org/10.1371/ journal.pone.0171937 AD Converter Cambridge Electronic Design Limited Micro 1401; https://ced.co.uk/produ cts/microwaveformio Constant Current Stimulator Digitimer DS7A; https://www.digitimer.c om/product/humanneurophysiology/periph eral-stimulators/ds7ads7ah-hv-currentstimulator/ Jo urn al P e-p roo f Plate Reader Tecan GENios; https://www.tecan.com/ customer-news/geniosan-innovativedetection-device-thatdelivers-flexibility-tothe-molecular-biologylaboratory-2347 Infrared Imaging System LI-COR Biosciences Odyssey Infrared Imaging System; RRID: SCR_023765; https://doi.org/10.1038/ s41598-024-70096-0 Cryostat Leica Biosystems CM1950; RRID: SCR_018061; https://www.leicabiosys tems.com/us/histologyequipment/cryostats/lei ca-cm1950/ Inverted Microscope Nikon Instruments ECLIPSE Ti inverted microscope system; RRID: SCR_021242; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Spinning Disk Confocal Microscope Yokogawa Electric Corporation CSU-22; RRID: SCR_020907; https://calm.ucsf.edu/c su-22-spinning-diskconfocal Jo urn al Pr e-p roo f

    Article Title: Atp13a5 Marker Reveals Pericyte Specification in the Mouse Central Nervous System
    Article Snippet: Rabbit polyclonal anti-NeuN , Millipore Sigma , ABN78.

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: For assessment of lesion pathology in the experimental animal model, the tissue sections of the thoracic spinal cord segments were incubated with Jo urn al Pr e-p ro f 58 diluted primary antibodies: rabbit polyclonal anti-NeuN (1:500; Millipore; Cat# ABN78; RRID: AB_10807945), mouse monoclonal anti-GFAP (1:500; Millipore; Cat# MAB360; RRID: AB_11212597), rabbit monoclonal anti-Vimentin (1:50; Cell Signaling Technology; Cat# 5741; RRID: AB_10695459), and rabbit polyclonal anti-GAP43 (1:500; Millipore; Cat# AB5220; RRID: AB_2107282).

    Article Title: SETD4 cells contribute to brain development and maintain adult stem cell reservoir for neurogenesis
    Article Snippet: The antibodies used in this study included mouse monoclonal anti-SETD4 (1:100, #sc-514060, Santa Cruz Biotechnology), rabbit polyclonal anti-GFAP (1:1000, #Z0334, Dako), goat polyclonal anti-GFAP (1:1000, #ab53554, Abcam), chicken polyclonal anti-NESTIN (1:1000, #NB100-1604, Novus Biologicals), mouse monoclonal anti-NESTIN (1:500, #MAB353, MilliporeSigma), rabbit polyclonal anti-SOX2 (1:200, #ab97959, Abcam), goat polyclonal anti-SOX2 (1:200, #AF2018, R&D Systems), rabbit monoclonal anti-KI67 (1:200, #ab16667, Abcam), mouse monoclonal antiPCNA (1:500, #ab29, Abcam), chicken polyclonal anti-GFP (1:1000, #ab13970, Abcam), rat monoclonal anti-BrdU (1:200, #ab6326, Abcam), goat polyclonal anti-DCX (1:500, #ab113435, Abcam), rabbit polyclonal anti-OLIG2 (1:500, #AB9610, MilliporeSigma), rabbit polyclonal anti-NEUN (1:1000, #ABN78, MilliporeSigma), mouse monoclonal antiMASH1 (1:200, #556604, BD Biosciences), chicken polyclonal anti-RFP (1:500, #600-901-379, Rockland immunochemicals), rabbit polyclonal anti-H4K20ME3 (1:500, #pAb-057-050, Diagenode), rabbit polyclonal antiH3K9AC (1:500, #ab10812, Abcam).

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: Rabbit Polyclonal Anti-NeuN , Millipore , Cat# ABN78; RRID: AB_10807945.

    Article Title: Disuse plasticity limits spinal cord injury recovery
    Article Snippet: For assessment of lesion pathology in the experimental animal model, the tissue sections of the thoracic spinal cord segments were incubated with diluted primary antibodies: rabbit polyclonal anti-NeuN (1:500; Millipore; Cat# ABN78; RRID: AB_10807945 ), mouse monoclonal anti-GFAP (1:500; Millipore; Cat# MAB360; RRID: AB_11212597 ), rabbit monoclonal anti-Vimentin (1:50; Cell Signaling Technology; Cat# 5741; RRID: AB_10695459 ), and rabbit polyclonal anti-GAP43 (1:500; Millipore; Cat# AB5220; RRID: AB_2107282 ).



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    Animal study verifying the effect of AE in mitigating EBI of SAH. (A) The complete timeline of an experimental plan, schematic diagram, and actual skull base image of the SAH/Sham model in this animal experiment. Some elements are created in BioRender (2026); https://BioRender.com/vwwir98 . (B) SAH grading score of each group in this stage. n = 24. (C) Modified Garcia score of each group, n = 24. (D) The water content of the left and right brains in different groups at this stage. n = 6. (E and F) Apoptosis of bleeding focus cortical neurons was detected by TUNEL assay (red), <t>NeuN</t> (green), and DAPI (blue) immunofluorescence. n = 6. Scale bars, 50 μm. (G) Bars show changes in BBs in SAH mice. n = 24. (H to N) Western blotting images and quantitative data of relative expression level of Il-1b, Il-6, Nos2, Il-4, Il-10, and Tgfb1 in the ipsilateral cortex after SAH. n = 6. (O) The coronal sections were stained with hematoxylin and eosin (H&E) for histological evaluation AE-regulated in SAH. n = 6. Scale bars, 50 μm. (P) Histopathological changes detected by Nissl’s staining in hippocampus and the hemorrhagic cortex. n = 6. Scale bars, 50 μm. In (B), (C), and (G), data were represented as median (interquartile range), and P values were calculated using Kruskal–Wallis H with Dunn test. In (D), (F), and (I) to (N), data were represented as mean ± SD, and P values were calculated using one-way ANOVA with Tukey multiple comparisons. * P < 0.05, ** P < 0.01, *** P < 0.001. The original Western blot images are shown in Data . Detailed experimental data and statistical results, including Z and P values for Kruskal–Wallis test and F and P values for ANOVAs, are provided in Data to .
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    Animal study verifying the effect of AE in mitigating EBI of SAH. (A) The complete timeline of an experimental plan, schematic diagram, and actual skull base image of the SAH/Sham model in this animal experiment. Some elements are created in BioRender (2026); https://BioRender.com/vwwir98 . (B) SAH grading score of each group in this stage. n = 24. (C) Modified Garcia score of each group, n = 24. (D) The water content of the left and right brains in different groups at this stage. n = 6. (E and F) Apoptosis of bleeding focus cortical neurons was detected by TUNEL assay (red), <t>NeuN</t> (green), and DAPI (blue) immunofluorescence. n = 6. Scale bars, 50 μm. (G) Bars show changes in BBs in SAH mice. n = 24. (H to N) Western blotting images and quantitative data of relative expression level of Il-1b, Il-6, Nos2, Il-4, Il-10, and Tgfb1 in the ipsilateral cortex after SAH. n = 6. (O) The coronal sections were stained with hematoxylin and eosin (H&E) for histological evaluation AE-regulated in SAH. n = 6. Scale bars, 50 μm. (P) Histopathological changes detected by Nissl’s staining in hippocampus and the hemorrhagic cortex. n = 6. Scale bars, 50 μm. In (B), (C), and (G), data were represented as median (interquartile range), and P values were calculated using Kruskal–Wallis H with Dunn test. In (D), (F), and (I) to (N), data were represented as mean ± SD, and P values were calculated using one-way ANOVA with Tukey multiple comparisons. * P < 0.05, ** P < 0.01, *** P < 0.001. The original Western blot images are shown in Data . Detailed experimental data and statistical results, including Z and P values for Kruskal–Wallis test and F and P values for ANOVAs, are provided in Data to .
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    Image Search Results


    Animal study verifying the effect of AE in mitigating EBI of SAH. (A) The complete timeline of an experimental plan, schematic diagram, and actual skull base image of the SAH/Sham model in this animal experiment. Some elements are created in BioRender (2026); https://BioRender.com/vwwir98 . (B) SAH grading score of each group in this stage. n = 24. (C) Modified Garcia score of each group, n = 24. (D) The water content of the left and right brains in different groups at this stage. n = 6. (E and F) Apoptosis of bleeding focus cortical neurons was detected by TUNEL assay (red), NeuN (green), and DAPI (blue) immunofluorescence. n = 6. Scale bars, 50 μm. (G) Bars show changes in BBs in SAH mice. n = 24. (H to N) Western blotting images and quantitative data of relative expression level of Il-1b, Il-6, Nos2, Il-4, Il-10, and Tgfb1 in the ipsilateral cortex after SAH. n = 6. (O) The coronal sections were stained with hematoxylin and eosin (H&E) for histological evaluation AE-regulated in SAH. n = 6. Scale bars, 50 μm. (P) Histopathological changes detected by Nissl’s staining in hippocampus and the hemorrhagic cortex. n = 6. Scale bars, 50 μm. In (B), (C), and (G), data were represented as median (interquartile range), and P values were calculated using Kruskal–Wallis H with Dunn test. In (D), (F), and (I) to (N), data were represented as mean ± SD, and P values were calculated using one-way ANOVA with Tukey multiple comparisons. * P < 0.05, ** P < 0.01, *** P < 0.001. The original Western blot images are shown in Data . Detailed experimental data and statistical results, including Z and P values for Kruskal–Wallis test and F and P values for ANOVAs, are provided in Data to .

    Journal: Research

    Article Title: Aerobic Exercise-Induced TGF-β Receptor Reprogramming Disrupts Neutrophil–Microglia Crosstalk to Attenuate Early Brain Injury after Subarachnoid Hemorrhage

    doi: 10.34133/research.1301

    Figure Lengend Snippet: Animal study verifying the effect of AE in mitigating EBI of SAH. (A) The complete timeline of an experimental plan, schematic diagram, and actual skull base image of the SAH/Sham model in this animal experiment. Some elements are created in BioRender (2026); https://BioRender.com/vwwir98 . (B) SAH grading score of each group in this stage. n = 24. (C) Modified Garcia score of each group, n = 24. (D) The water content of the left and right brains in different groups at this stage. n = 6. (E and F) Apoptosis of bleeding focus cortical neurons was detected by TUNEL assay (red), NeuN (green), and DAPI (blue) immunofluorescence. n = 6. Scale bars, 50 μm. (G) Bars show changes in BBs in SAH mice. n = 24. (H to N) Western blotting images and quantitative data of relative expression level of Il-1b, Il-6, Nos2, Il-4, Il-10, and Tgfb1 in the ipsilateral cortex after SAH. n = 6. (O) The coronal sections were stained with hematoxylin and eosin (H&E) for histological evaluation AE-regulated in SAH. n = 6. Scale bars, 50 μm. (P) Histopathological changes detected by Nissl’s staining in hippocampus and the hemorrhagic cortex. n = 6. Scale bars, 50 μm. In (B), (C), and (G), data were represented as median (interquartile range), and P values were calculated using Kruskal–Wallis H with Dunn test. In (D), (F), and (I) to (N), data were represented as mean ± SD, and P values were calculated using one-way ANOVA with Tukey multiple comparisons. * P < 0.05, ** P < 0.01, *** P < 0.001. The original Western blot images are shown in Data . Detailed experimental data and statistical results, including Z and P values for Kruskal–Wallis test and F and P values for ANOVAs, are provided in Data to .

    Article Snippet: After fixation, the tissue was processed for TUNEL assay with the cell apoptosis detection kit (Servicebio, G1502-50T), and Rabbit anti-NeuN polyclonal antibody (ABclonal, A0951) and its corresponding secondary antibody: Donkey anti-rabbit IgG conjugated to Alexa Fluor 488 (Antgene, ANT024, diluted 1:400).

    Techniques: Modification, TUNEL Assay, Immunofluorescence, Western Blot, Expressing, Staining

    FGE mitigates METH-induced neuronal loss in the mouse hippocampus. (A) Representative immunohistochemical images of NeuN expression in the hippocampus CA1, CA3, and DG regions across different groups. Red arrows indicate examples of pyknotic or damaged neurons. (B–D) Quantitative analysis of the number of NeuN-positive cells in the hippocampus CA1, CA3, and DG regions. * p < 0.05, ** p < 0.01, *** p < 0.001; # p < 0.05, ## p < 0.01, ### p < 0.001, & p < 0.05, && p < 0.01, &&& p < 0.001.

    Journal: Frontiers in Medicine

    Article Title: Gastrodia elata fermentation alleviates methamphetamine-induced neuroinflammation and anxiety- and depression-like behaviors by regulating the PI3K-AKT signaling pathway

    doi: 10.3389/fmed.2026.1768944

    Figure Lengend Snippet: FGE mitigates METH-induced neuronal loss in the mouse hippocampus. (A) Representative immunohistochemical images of NeuN expression in the hippocampus CA1, CA3, and DG regions across different groups. Red arrows indicate examples of pyknotic or damaged neurons. (B–D) Quantitative analysis of the number of NeuN-positive cells in the hippocampus CA1, CA3, and DG regions. * p < 0.05, ** p < 0.01, *** p < 0.001; # p < 0.05, ## p < 0.01, ### p < 0.001, & p < 0.05, && p < 0.01, &&& p < 0.001.

    Article Snippet: SP Rabbit/Mouse HRP Kit (DAB) (Cat# SA00001-2/SA00001-1, CWBIO, China); DAB Substrate Kit (Cat# ZLI-9018, Zhongshan Jinqiao, Beijing, China); SPARKscript II RT Kit/SYBR Green RT-PCR Kit (Cat# AG0305-B/AH0104-B, SparkJade, China); Anti-Iba1 Rabbit Polyclonal Antibody (Cat# F155306, Abways, China); Anti-GFAP Rabbit Polyclonal Antibody (Cat# CY5424, Abcam, United Kingdom); Anti-NeuN Rabbit Polyclonal Antibody (Cat# 26975-1-AP, Proteintech, China); AKT/phosphorylated AKT (p-AKT)/phosphorylated PI3K (p-PI3K)/GAPDH (Cat# 10176-2-AP/66444-1-IG/20584-1-AP/60004-1-IG, Proteintech, China); PI3K (Cat# AF3242, Affinity, China).

    Techniques: Immunohistochemical staining, Expressing